Journal of Life Science Agriculture and Technology

Effects of Temperature and Storage Duration on the Quality of Eggs Produced by Free-Range Laying Hens

kanok Chaovapasee — 2026-06-11

Temperature and egg storage duration play an important role in determining the shelf life of eggs. This study aimed to investigate the effects of egg storage temperature and egg storage duration on the quality of eggs produced by commercial laying hens raised under free-range conditions. This study employed a 2 × 8 factorial experiments arranged in a completely randomized design. The first factor was egg storage temperature, and the second factor was egg storage duration. There were five replications for each treatment combination. Data were collected on egg weight, percentage weight loss, egg shape, and egg quality. The results showed that the interaction between egg storage temperature and egg storage duration significantly affected yolk height, yolk diameter, and yolk index (P < 0.05). Egg storage temperature significantly affected percentage egg weight loss, albumen height, and Haugh unit (P < 0.05), whereas egg storage duration significantly affected percentage egg weight loss (P < 0.05). These findings indicate that refrigerated storage slowed the deterioration of egg quality in eggs produced by free-range laying hens more effectively than storage at room temperature under actual conditions.

A study of optimal method and condition to extract essention oil of Litsea glutinosa leaves from Tumbon Khok Khian, Mueang, Narathiwat

Suphaporn Tesvichian — 2026-06-13

Litsea glutinosa (Lour.) is a local plant found in the lower southern region of Thailand. Essential oil derived from L. glutinosa possesses medicinal properties but is relatively expensive. In order to reduce the cost of importing essential oils and to lower production costs, this research aimed to investigate the most suitable method for extracting essential oil from Litsea leaves with the highest efficiency. Four different extraction methods were compared: Ethanol Extraction (soaking in 95% ethanol), Accelerated Solvent Extraction, Steam Distillation, and Hydro Distillation. The results showed that the most optimal condition was Hydro Distillation of L. glutinosa, using a ratio of 500 grams of dried L. glutinosa leaves to 3,500 milliliters of distilled water, for a duration of 3 hours. This yielded 1 milliliter of essential oil, representing an essential oil yield of 0.2% (v/w). This method is low-cost and well-suited for further application in the health and personal care industry.

Optimization of Formulation Ratios for Snack Production from Mushroom Stem By-Products to Support Entrepreneurial Development

Rugchanok Puwaphut — 2026-06-28

This research aimed to determine the optimal formulation ratios for processing oyster mushroom stems, a by-product from mushroom processing factories, into snack products to add value and support entrepreneurial development. Four snack formulations were developed and evaluated through sensory analysis conducted by 30 panelists. The nutritional compositions of each formulation was also analyzed. The results indicated that formulation 3 achieved the highest consumer acceptance, with 25 out of 30 panelists expressing a preference for it due to its favorable taste, aroma, and texture. Nutritional analysis of a 30 g serving revealed an energy content of 110 kcal, a protein content of 3 g, a total fat content of 2.5 g, and a sodium content of 150 mg. These findings demonstrate that oyster mushroom stems have strong potential as a functional ingredient in snack production, offering nutritional benefits and serving as a healthier snack alternative. In addition, the utilization of mushroom stem by-products contributes to agricultural waste valorization and creates opportunities for sustainable entrepreneurial development.

Development of Fermented Cassava Composite Flour with Germinated Brown Rice Powder and Rice Flours: Physicochemical Properties, Bioactive Compounds, Functional Properties and Microbiological Qualities

Santat Sinjaroonsak — 2026-06-28

This study aimed to develop composite flour from fermented cassava combined with germinated brown rice and rice flours, and to evaluate its physicochemical properties, bioactive compounds, functional properties, proximate composition, mineral content, and microbiological quality. Fresh cassava roots were washed, peeled, cut, soaked, boiled, and fermented with 5% (w/w) Rhizopus oligosporus starter culture for 36 h. The fermented cassava was then dried at 60°C for 3 days and milled into fermented cassava flour. The flour was used to formulate three composite flour blends: fermented cassava flour with white rice flour and germinated brown rice powder; fermented cassava flour with glutinous rice flour and germinated brown rice powder; and fermented cassava flour with white rice flour, glutinous rice flour, and germinated brown rice powder. These formulations were compared with fermented cassava flour, commercial cassava flour, and commercial all-purpose wheat flour. The developed composite flours had moisture contents of 7.75–9.12%, pH values of 5.31–5.56, and significantly higher ash contents than the commercial flours (p<0.05). The developed flours showed higher DPPH radical scavenging activity than the commercial flours, with IC₅₀ values ranging from 272.69 to 293.86 mg/mL and GABA contents of 0.717–0.799 mg/g. Proximate analysis showed that the developed flours contained 4.59–5.68 g/100 g protein and 2.50–3.39 g/100 g crude fiber, with phosphorus, magnesium, calcium, iron, and zinc as mineral components. Microbiological analysis showed total viable counts at the 10³ CFU/g level, yeast and mold counts of <100/g, and no detection of Salmonella sp., Staphylococcus aureus, or Bacillus cereus in all formulations. However, the developed flours had lower lightness values than the commercial flours. These findings indicate that fermented cassava flour combined with germinated brown rice powder incorporation is a promising approach for developing functional composite flour with improved bioactive and nutritional properties.

Effects of Buserelin (Suprefact E) Hormone on Seminal Vesicle Physical Characteristics, Gonadosomatic Index and Semen Volume in Male Catfish (Clarias gariepinus)

Taufik Hayimad — 2026-06-30

This research aims to study the efficacy of the Buserelin (Suprefact E) hormone on the physical characteristics of the reproductive system in male African catfish. The experiment was conducted on mature male African catfish, which were divided into 3 experimental groups: 1) No hormone injection, 2) Injection of the synthetic hormone Suprefact E without a synergist, and 3) Injection of the synthetic hormone Suprefact E with a synergist. The hormone was injected intramuscularly, and the results were monitored over a period of 12-15 hours. The results revealed that the average Gonadosomatic Index (GSI) values were 0.54 ± 0.14^a, 0.51 ± 0.15, and 0.92 ± 0.22 %, respectively. It was found that experimental group 3 showed a highly significant statistical difference compared to groups 1 and 2 (P<0.01). The study of the physical characteristics of the seminal vesicles showed that the color and appearance in experimental group 1 were predominantly clear white and slender. Group 2 was predominantly clear white and long-slender. Group 3 was predominantly cloudy white and plump. The average lengths of the seminal vesicles were 5.35 ± 0.50, 6.03 ± 1.10, and 6.21 ± 0.77 centimeters, respectively. There was no statistically significant difference among the 3 experimental groups (P>0.05). Additionally, the study on the physical characteristics of the semen revealed average semen volumes of 1.53 ± 0.50, 1.81 ± 0.57, and 3.21 ± 0.35 cc, respectively. Experimental group 3 showed a highly significant statistical difference compared to groups 1 and 2 (P<0.01). Regarding semen color and quantity, the third treatment showed significantly different in both aspects with milky and creamy color and highest quantity of semen which indicate that the semen is matured whereas the others treatment showed relatively clear and small amount of semen. The findings of this study demonstrate that the application of the Buserelin (Suprefact E) hormone in male African catfish is viable, which could serve as an important approach to enhancing commercial breeding efficiency in the future.