Effect of Culture Media and Explant Types on Micropropagation of Karanda (Carissa carandas L.) In Vitro สุจิตรา สืบนุการณ์ และนงลักษณ์ พยัคฆศิรินาวิน

Abstract

This research aimed to find suitable explants and appropriate concentration of BA for Karanda propagation by tissue culture. The experiment was based on 2×6 Factorial in Completely Randomized Design (Factorial in CRD) with 12 treatments and 10 replications in each treatment. Apical and axillary buds were cultured on MS media with BA concentrations at 0, 1, 3, 5, 7, and 9 ppm. The results were revealed that the most suitable explant was apical bud. This was due to the best results of average height, duration for new shoots emergence, average shoots per explant and survival percentage. When the relationship between the explant and growth regulator were considered, it was found that culturing the Karanda apical shoots on MS media with 7 ppm BA concentration was most suitable. This was due to the best shoot development (2.75 cm.), duration for new shoots emergence (7 days), the average new shoot height (1.18 cm), and surviving rate (70%). The average new shoot number was moderate (3 shoot per explant), however. This was followed by culturing apical shoot in MS media with 3 ppm BA. This resulted in the average new shoot number of 3.37 shoots per explant, the surviving rate of 70%, moderate average new shoot height of 0.91 cm. The use of axillary bud with MS media with 9 ppm BA concentration was not recommended because of the lowest surviving rate of 40% and no shoot development.