Effect of coconut water and BA on growth induction of Cannabis sativa L. in vitro. อรพรรณ หัสรังค์ กษิดิ์เดช อ่อนศรี บัญญัติ เศรษฐฐิติ และเจษฎา ตะราศี

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Abstract

This experiment aimed to study on effect of coconut water and BA on growth induction of Cannabis sativa L. in vitro. Explants of cannabis were cultured on Semi-solid Murashige and Skoog (MS) media with the addition of 30 g./L., sugar and 8 g./L. agar as well as the media was adjusted pH 5.7. The experiment was designed in Completely Randomized Design (CRD) with 7 factor such as MS, MS with BA concentrations (3, 4 and 5 mg./L.) and MS with Coconut water 20% including BA concentrations (3, 4 and 5 mg./L.) for 8 weeks. Every factor had 10 repetitions with 1 plant in each. The result showed that the media without coconut water had better growth induction of cannabis explants under aseptic conditions than the media with coconut water. The MS media with 5 mg./L. BA had the best average number of shoot (3 shoots). On the other hand, the MS media with 3 mg./L BA had the most average height (3.18 cm.) and number of leave (12.25 leaves). In sum, the coconut water does not affect on growth induction of Cannabis explants, but BA concentration has effect on it from this experiment.

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