Impact total of harvesting age on phenolic content, flavonoid level, and antioxidant capacity in Justicia gendarussa leaves
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Abstract
This study evaluated the influence of harvesting age on the total phenolic content, flavonoid levels, and antioxidant properties of Justicia gendarussa leaves. Significant differences were observed across different leaf ages (3, 4, 5, and 6 months). The highest phenolic content was recorded in leaves harvested at four months (1.769 mg GAE/g DW), while the six-month-old leaves exhibited the highest concentration of flavonoids (27.505 mg QE/g DW). The antioxidant capacity peaked in the three-month-old leaves (8.1985 µmol TE/g DW) using the DPPH method, while six-month-old leaves displayed the greatest antioxidant potential (12.8684 µmol TE/g DW) measured via the FRAP method. Therefore, the six-month-old leaves were found to have the optimal combination of high flavonoid content and antioxidant activity as determined by the FRAP method.
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