Amino acid composition of beef depending on the breed and age of dairy bulls
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Abstract
The quality composition of beef is ensured by a significant variation in the content of essential and non-essential amino acids and their ratios and depends on many factors, the most important of which are the breed and age of the animals. They contribute to the accumulation of individual amino acids in their bodies and determine the quantity and quality of the protein formed. The study identified 16 amino acids in the longest back muscle of bulls from the studied breeds, 9 of which are essential and 7 are nonessential. Certain breeds found to be an advantage in the amino acid composition of beef during the age range of bulls from 3 to 21 months, due to breed-specific features of the fractional composition of muscle proteins formed during a certain period of their growth. Among the essential amino acids, lysine showed the dominant amino acid, accounting for 5.02-7.85% of the total mass fraction of protein. Among the nonessential amino acids, the content of glutamine varied from 10.93% to 12.66% of the mass fraction of protein. The significant interbreed was differed in the content of amino acids such as lysine, histidine, arginine, serine, proline, alanine, methionine, leucine, and tyrosine result in the formation of specific qualities and biological values of beef protein for each studied breed.
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References
Aliiev, E., Paliy, A., Kis, V., Paliy, A., Petrov, R., Plyuta, L., Chekan, O., Musiienko, O., Ukhovskyi, V. and Korniienko, L. (2022). Establishment of the influence of technical and technological parameters of dairy and milking equipment on the efficiency of machining. Eastern-European Journal of Enterprise Technologies, 1:44-55.
Applegate, T. J. and Angel, R. (2008). Protein and amino acid requirements for poultry. Purdue University Extension Publication AS-581-W.
Bischof, G., Witte, F., Terjung, N., Januschewski, E., Heinz, V., Juadjur, A. and Gibis, M. (2022). Effect of sampling position in fresh, dry-aged and wet-aged beef from M. Longissimus dorsi of Simmental cattle analyzed by 1H NMR spectroscopy. Food Research International, 156:111334.
Cai, Z. W., Zhao, X. F., Jiang, X. L., Yao, Y. C., Zhao, C. J. and Xu, N. Y. (2010). Comparison of muscle amino acid and fatty acid composition of castrated and uncastrated male pigs at different slaughter ages. Italian Journal of Animal Science, 9:123-129.
Cho, S., Seol, K., Kang, S., Kim, Y., Seo, H., Lee, W., Kim, J. and Ba, V. H. (2020). Comparison of tastes-related components and eating quality between hanwoo steer and cow longissimus thoracis muscles. Food Science of Animal Resource, 40:908-923.
Christensen, M., Ertbjerg, P., Failla, S., Sañudo, C., Richardson, R. I., Nute, G. R., Olleta, Panea, J. L. B., Albertí, P., Juárez, M., Hocquette, J. F. and Williams, J. L. (2011). Relationship between collagen characteristics, lipid content and raw and cooked texture of meat from young bulls of fifteen European breeds. Meat Science, 87:61-65.
Di Luca, A., Elia, G., Mullen, A. M. and Hamill, R. M. (2013). Monitoring postmortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate. Proteome Science, 11:9-18.
Farionik, T. V., Yaremchuk, O. S., Razanova, O. P., Ohorodnichuk, G. M., Holubenko, T. L. and Glavatchuk, V. А. (2023). Effects of mineral supplementation on qualitative beef parameters. Regulatory Mechanisms in Biosystems, 14:64-69.
Florek, M., Drozd, L., Skatecki, P., Domaradzki, P., Litwiñczuk, A. and Tajchman, K. (2017). Proximate composition and physicochemical properties of European beaver (Castor fiber L.) meat. Meat Science, 123:8-12.
Guerrero, A., Valero, M. V., Campo, M. M. and Sanudo, C. (2013). Some factors that affect ruminant meat quality: from the farm to the fork. Review. Acta Scientiarum. Animal Science, 35:335-347.
Hanzelkova, S., Simeonovova, J., Hampel, D., Dufek, A. and Subrt, J. (2011). The effect of breed, sex and aging time on tenderness of beef meat. Acta Veterinaria Brno, 80:91-196.
Hollo, G., Csapo, J., Szucs, E., Tozser, J., Repa, I. and Hollo, I. (2001). Influence of breed, slaughter weight and gender on chemical composition of beef. Part 1. Amino acid profile and biological value of proteins. Asian-Australasian Journal of Animal Sciences, 14:1555-1559.
Hollo, G., Nuernberg, K., Hollo, I., Csapo, J., Seregi, J., Repa, I. and Ender, K. (2007). Effect of feeding on the composition of longissmus muscle of Hungarian Grey and Holstein Friesian bulls. III. Amino acid composition and mineral content. Archiv fur Tierzucht, 50:575-586.
Jacques, J., Berthiaume, R. and Cinq-Mars, D. (2011). Growth performance and carcass characteristics of Dorset lambs fed different concentrates: forage ratios or fresh grass. Small Ruminant Research, 95:113-119.
Jayasena, D. D., Ahn, D. U., Nam, K. C. and Jo, C. (2013). Factors affecting cooked chicken meat flavour: A review. World’s Poultry Science Journal, 69:515-526.
Jeffer, S. B., Kassem, I. I., Kharroubi, S. A. and Abebe, G. K. (2021). Analysis of Food Safety Management Systems in the Beef Meat Processing and Distribution Chain in Uganda. Foods, 10:2244.
Jeong, D., Oh, M. R., Seong, P. N., Cho, S., Kang, G. H., Kim, J. H., Jeong, S. G., Lee, J. S. and Park, B. Y. (2012). Comparison of meat quality traits, free amino acid and fatty acid on longissimus lumborum muscles from Hanwoo, Holstein and Angus steers, fattened in Korea. Korean Journal for Food Science of Animal Resources, 32:591-597.
Kim, Y. H. B., Kemp, R. and Samuelsson, L. M. (2016). Effects of dry aging on meat quality attributes and metabolite profiles of beef loins. Meat Science, 111:168-176.
Kodani, Y., Miyakawa, T., Komatsu, T. and Tanokura, M. (2017). NMR-based metabolomics for simultaneously evaluating multiple determinants of primary beef quality in Japanese Black cattle. Scientific Reports, 7:1-13.
Koutsidis, G., Elmore, J. S., Oruna-Concha, M. J., Campo, M. M., Wood, J. D. and Mottram, D. S. (2008). Water-soluble precursors of beef flavour: I. Effect of diet and breed. Meat Science, 79:124-130.
Lee, S. H., Kim, C. N., Ko, K. B., Park, S. P., Kim, H. K., Kim, J. M. and Ryu, Y. C. (2019). Comparisons of beef fatty acid and amino acid characteristics between jeju black cattle, hanwoo, and wagyu breeds. Food Science of Animal Resources, 39:402-409.
Lopesa, A. F., Cristina, I. M., Alfaiaa, M., Ana, I., Partidariob, C., Lemosa, J. P. and Prates, J. M. (2015). Influence of household cooking methods on amino acids and minerals of Barrosa-PDO veal. Meat Science, 99:38-43.
Lopez, M. J. and Mohiuddin, S. S. (2023). Biochemistry, Essential Amino Acids. 2023 Mar 13. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan–. PMID: 32496725
Mylostуva, D. F., Farafonov, S. J., Puzniak, O. М., Stakhiv, V. І., Borshchenko, V. V., Tsisinska, S. V. and Voloshin, S. V. (2023). Antioxidant system of the body of young Ukrainian beef cattle under the action of microelements. Regulatory Mechanisms in Biosystems, 14:106-111.
Nanka, O., Shigimaga, V., Paliy, A., Sementsov, V. and Paliy, A. (2018). Development of the system to control milk acidity in the milk pipeline of a milking robot. Eastern-European Journal of Enterprise Technologies, 3/9:27-33.
Nelson, D. L. and Cox, M. M. (2008). Lehninger principles of biochemistry. 2021. 5-th Ed, W. H. Freemanand Company 41 Madison Avenue NewYork, NY 1001.
Nicastro, F., Gallo, R., Caputi, A. and Zezza, I. (2000). Influence of diet on amino acid profiles of two muscle in chianina beef. Journal of Animal Science, 83:45-55.
Paliy, A., Sumakova, N., Petrov, R., Shkromada, O., Ulko, L. and Palii, A. (2019). Contamination of urbanized territories with eggs of helmiths of animals. Biosystems Diversity, 27:118-124.
Poberezhets, J. M., Yaropud, V. M., Kupchuk, I. M., Kolechko, A. V., Rutkevych, V. S., Hraniak, V. F., Burlaka, S. A. and Voitsitskyi, О. V. (2023). Efficiency of a food supplement containing Saccharomyces cerevisiae culture in the diet of broiler chickens. Regulatory Mechanisms in Biosystems, 14:354-357.
Reicks, A. L., Brooks, J. C., Garmyn, A. J., Thompson, L. D., Lyford, C. L. and Miller, M. F. (2011). Demographics and beef preferences affect consumer motivation for purchasing fresh beef steaks and roasts. Meat Science, 87:403-411.
Reznikov, O. G. (2003). General ethical principles of experiments on animals. The first national congress on bioethics. Endocrinology, 8/1:142-145.
Robert, R. W., Jamie, I. B., Carlene, S. С. and Paul, J. M. (2018). Factors contributing to the selection of dietary protein food sources. Clinical Nutrition, 37:130-138.
Rodionova, K., Paliy, A. and Кhimych, M. (2021). Veterinary and sanitary assessment and disinfection of refrigerator chambers of meat processing enterprises. Potravinarstvo Slovak Journal of Food Sciences, 15:616-626.
Scollan, N. D., Costa, P., Hallett, K. G., Nute, G. R., Wood, J. D. and Richardson, R. I. (2006). The fatty acid composition of muscle fat and relationships to meat quality in Charolais steers: influence of level of red clover in the diet. Proceedings of the British Society of Animal Science, 23.
Simmonds, R. C. (2017). Chapter 4. Bioethics and animal use in programs of research, teaching, and testing. In: Weichbrod, R. H., Thompson, G. A. H., Norton, J. N. (Eds.). Management of animal care and use programs in research, education, and testing. 2nd edition. CRC Press, Taylor and Francis, Boca Raton. pp.1-28.
Strasbourg. (1986). European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. European Treaty Series No. 123, Protection of Vertebrate Animals, 18.III.1986.
Šubrt, J., Kráčmar, S. and Diviš, V. (2002). The profile of amino acids in intramuscular protein of bulls of milked and beef commercial types. Czech Journal of Animal Science, 47:21-29.
Van Elswyk, M. E. and McNeill, S. H. (2014). Impact of grass/forage feeding versus grain finishing on beef nutrients and sensory quality: the U.S. experience. Meat Science, 96:535-540.
Watanabe, A., Ueda, Y. and Higuchi, M. (2004). Effects of slaughter age on the levels of free amino acids and dipeptides in fattening cattle. Journal Animal Science, 75:361-367.
Williams, P. and Droulez, V. (2010). Australian red meat consumption and implications of changes over 20 years on nutrient composition. Food Australia, 62:87-94.
Yaremchuk, O. S., Razanova, O. P., Skoromna, O. I., Chudak, R. A., Holubenko, T. L. and Kravchenko, O. O. (2022). Post-slaughter indicators of meat productivity and chemical composition of the muscular tissues of bulls receiving corrective diet with protein-vitamin premix. Regulatory Mechanisms in Biosystems, 13:219-224.
Yermishev, O. (2023). Ecological and toxicological features of the effect of stable cesium on striated muscle tissue (myocardium and skeletal muscles) of mammals. Regulatory Mechanisms in Biosystems, 14:415-423.
Zavgorodnii, A. I., Pozmogova, S. A., Kalashnyk, M. V., Paliy, A. P., Plyuta, L. V. and Palii, A. P. (2021). Etiological factors in triggering non-specific allergic reactions to tuberculin in cattle. Regulatory Mechanisms in Biosystems, 12:228-233.