Successful production of Goat (Capra hircus) embryos: effects of cysteamine on maturation of goat oocytes and subsequent embryonic development following fertilization in-vitro
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Abstract
The results showed no significant differences in percent maturation of goat oocytes among control, 50 uM, and 100 µM cysteamine groups (81.97+7.11, 81.46+ 5.28 and 84.44+3.03), respectively. This implies that the addition of IVM medium with cysteamine of goat oocytes matured in-vitro are not expressed at nuclear maturation level. However, following in-vitro fertilization, the cleavage rate of 100 µM cysteamine (50.00+2.40) was significantly higher than control and 50 µM (42.40 + 3.27, 43.72+ 2.77), respectively. Similarly, the blastocyst formation rate of 100 µM cysteamine (28.33+19.99) was significantly higher than those of control and 50 µM (19.84+6.91 and 19.64+6.18), respectively. The results indicated that the addition of 100 µM cysteamine into IVM medium is found to be beneficial in improving the cleavage rate of goat oocytes and subsequent development in-vitro, suggesting huge potential of the IVF technology in enhancing production and genetic improvement in goat species.
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