Chromosome and karyotype analysis of Dendrobium spp.
Article Sidebar
Main Article Content
Abstract
Cytogenetics is a crucial aspect in determining the success of breeding programs for orchid plants. Chromosome contains DNA, which is the genetic information for each species. The characterized orchid chromosomes were Dendrobium lamelatum, Dendrobium macrophyllum, Dendrobium mirbelianum, Dendrobium purpureum, and Dendrobium secundum. The results showed that all Dendrobium orchids had a metacentric shape with 2n = 38 chromosomes, except for D. purpureum with 2n = 40. The size of each arm of D. lamellatum, D. macrophyllum, D. mirbelianum, D. purpureum, and D. secundum is 2.51 ± 0.55 µm to 2.67 ± 0.54 µm, 1.54 ± 0.41 µm to 1.86 ± 0.37 µm, 5.28 ± 0.16 µm to 6.31 ± 0.17 µm, 2.45 ± 0.60 µm to 2.62 ± 0.64 µm, and 2.70 ± 0. 49 µm to 2.89 ± 0.51 µm. The index value (A1) in all species showed a metacentric shape, while the index value (A2) in all species showed a deviation in chromosome size is small.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Aoyama, M., Alonso, C. and Baltierra, X. C. (2013). Chromosome numbers of some terrestrial orchids in Chile. Chromosome Botany, 8:23-27.
Apriliyania, R. and Wahidah, B. F. (2021). Propagation of Dendrobium sp. in vitro: success factors. Phylogeny: Journal Biology Student, 1:33-46.
Atoche, J. A., Iiyama, C. M. and Cardoso, J. C. (2021). Polyploidization in orchids: from cellular changes to breeding applications. Plants, 11:1-21.
Bunnag, S. and Hongthongkham, J. (2015). Polyploid induction of Dendrobium draconis Rchb.f. Acta Horticulture, 1087:445-451.
Chen, J., Xia, N., Wang, X., Beeson Jr., R. C. and Chen, J. (2017). Ploidy level, karyotype, and DNA content in the Genus Lonicera. HortScience, 52:1680-1686.
Chen, WH., Kao, YL., Tang, CY. Tsai, C. C. and Lin, T. Y. (2013). Estimating nuclear DNA content within 50 species of the genus Phalaenopsis Blume (Orchidaceae). Scientia Horticulture, 161:70-75.
Choi, B., Gang, G. H., Kim, H., Byun, H., Kwak, M., So, S., Myeong, H. H. and Jang, T. S. (2021). Cytological study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An endangered species from Korea. Plants, 10:1-10.
De, L. C., Rao, A. N., Rajeevan, P. K., Srivastava, M. and Chhetri, G. (2015). Morphological characterization in Dendrobium species. Journal of Global Biosciences, 4:1198-1215.
Eroglu, H. E. (2015). Which chromosomes are subtelocentric or acrocentric? A new karyotype symmetry/asymmetry index. Caryologia: International Joyrnal of Cytology, 68:239-245.
Grosso, V., Farina, A., Giorgi, D., Nardi, L., Diretto, G. and Lucretti, S. (2018). A high-throughput flow cytometry system for early screening of in vitro made polyploids in Dendrobium hybrids. Plant Cell Tissue Organ Culture, 132:57-70.
Hartati, S., Darsana, L. and Cahyono, O. (2014). Study of cytological characterization of orchids in the context of preserving germplasm. Caraka Tani 29:25-30.
Hartati, S., Nandariyah., Yunus, A. and Djoar, D. W. (2017). Short communication: Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley). Biodiversity, 18:555-559.
Hartati, S., Parjanto., Sukaya., Muliawati, E. S., Nandariyah., Yuniastuti, E., Manurung, I. R. and Purmiyoto, C. W. W. (2022a). Chromosome of Phaius tankervilleae and Phaius amboinensis Orchid. International Conference on Sustainable Agriculture and Environment, 1114:1-5.
Hartati, S., Sukaya and Samanhudi (2022b). Karyotype and ploidy of Vanda dearei and Vanda celebica orchid using flow cytometry analysis. Journal Indonesian Agronomy, 50:337-342.
Hsu, C. C., Chen, S. Y., Lai, P. H., Hsiao, Y. Y., Tsai, W. C., Liu, Z. J., Chung, M. C., Panaud, O. and Chen, H. H. (2020). Identification of high-copy number long terminal repeat retrotransposons and their expansion in Phalaenopsis orchids. BMC Genomics, 21:1-13.
Huy, N. P., Tam, D. T., Luan, V. M., Hoang, T. T., Hien, V. T., Ha, N., Duy, P. N. and Tan, N. D. (2019). In vitro polyploid induction of Paphiopedilum villosum using colchicine. Scientia Horticulture, 252:283-290.
Kondo, H., Phlaetita, W., Mii, M., Kikuchi, S., Deguchi, A. and Miyoshi, K. (2020). Efficient chromosome doubling of an interspecific hybrid Dendrobium Stardust 'fire bird' by treatment of amiprofos-methyl to protocorm like body. Biotechnology, 56:738-749.
Kurniawan, F. Y. and Riyadi, A. D. (2021). Morphological and chromosomal characterization of the orchid Peristylus goodyeroides Lindl. From Curug Setawing, Kulonprogo. Journal Math and Science Education, 12:110-122.
Kursel, L. E. and Malik, H. S. (2016). Centromere. Current Biology, 26:481-492.
Lalla, M. and Sudiarta, I. M. (2022). Development of orchid plants in the Motilango Pine Forest Tourism Area, Tibawa District, Gorontalo Regency. J Community Service, 3:87-91.
Lee, Y. I., Chang, F. C. and Chung, M. C. (2011). Chromosome pairing affinities in interspecific hybrids reflect phylogenetic distances among lady's slipper orchids (Paphiopedilum). Annals of Botany, 108:113-121.
Lee, Y. I., Tseng, Y. F., Lee, Y. C. and Chung, M. C. (2020). Chromosome constitution and nuclear DNA content of Phalaenopsis hybrids. Scientia Horticulture, 262:1-9.
Liu, Y., Duan, S. D., Jia, Y., Hao, L. H., Xiang, D. Y., Chen, D. F. and Niu, S. C. (2023). Polyploid induction and karyotype analysis of Dendrobium officinale. Horticlturae, 9:329.
Moraes, A. P., Koehler, S., Cabral, J. S., Gomes, S. S. L., Viccini, L. F., Barros, F., Felix, L. P., Guerra, M. and Martins, E. R. F. (2017). Karyotype diversity and genome size variation in Neotropical Maxillariinae orchids. Plant Biology, 19:298-308.
Muliawati, E., Hartati, S., Parjanto. Sukaya., Nandariyah., Yuniastuti, E., Manurung, I. R. and Purmiyoto, C. W. W. (2023). Karyotype of Phaius tankervilleae and Phaius amboinensis orchid. E3S Web of Conferences, 373:1-6.
Neto, E. M., Nollet, F., Moraes, A. P. and Felix, L. P. (2017). Intrachromosomal karyotype asymmetry in Orchidaceae. Genetics and Molecular Biology, 40:610-619.
Niu, Z., Zhu, F., Fan, Y., Li, C., Zhang, B., Zhu, S., Hou, Z., Wang, M., Yang, J., Xue, Q., Liu, W. and Ding, X. (2021). The chromosome-level references genome assembly for Dendrobium officinale and its utility of functional genomics research and molecular breeding study. Acta Pharmaceutica Sinica B, 11:2080-2092.
Nugraheni, K. A., Kurniawan, F. Y., Riyadi, A. D. R., Kinasih, A., Ilmam, H. P. L. and Semiarti, E. (2022). Cytological analysis of Aerides odorata Lour. from Sleman, Special Region of Yogyakarta. Advances in Biological Sciences Research, 22:545-552.
Ramesh, T., Kumari, K. G., Muruganantham, P. and Vanithamani, J. (2022a). Cytological studies on some endemic terrestrial orchids. Plant Archives, 22:151-157.
Ramesh, T., Kumari, K. G., Muruganantham, P., Praveena, R., Vanithamani, J. and Koperuncholan, M. (2022b). Studies on chromosome variation in Vanda species of Orchidaceae. Plant Archives, 22:130-135.
Saha, M. L. (2022). Botany: Addressing the challenges and opportunities for the 21st century. Dhaka (BD): SRL Printing Press.
Sharma, H. B. and Bhattacharyya, D. (2023). Karyomorphological studies of some lithophytic species of the genus Dendrobium Sw. (Orchidaceae) from Manipur, India: a cytotaxonomic approach. Nucleus, 66:145-150.
Sharma, S. K. and Mukai, Y. (2015). Chromosome research in orchids: current status and future prospects with special emphasis from molecular and epigenetic perspectives. Nucleus, 58:173-184.
Silva, G. M., Varella, T. L., Karsbug, I. V., Santana, T. N., Carvalho, I. F., Anez, R. B. S. and Rossi (2017). Cytogenetic characterization of species and hybrids of orchids of Cattleya genus. Ctytologia, 82:137-140.
Wang, Y., Chen, Y., Wei, Q., Chen, X., Wan, H. and Sun, C. (2022). Characterization of repetitive sequences in Dendrobium officinale and comparative chromosomal structures in Dendrobium species using FISH. Gene, 846:1-12.
Yenchon, S. and Te-chato, S. (2014). Polyploid induction of Dendrobium formosum by colchisine treatment in vitro. Acta Horticulture, 1025:81-88.
Yuan, Y., Liu, X., Wang, J. and Zhang, J. (2018). Morphological and microscopic identification of three major medicinal Dendrobium species in the Ta-pieh Mountains area. Microscopy Research Technique, 1-11.
Zhang, Y., Zhang, G. Q., Zhang, D. Liu, X. D., Xu, X. Y., Sun, W. H., Yu, X., Zhu, X., Wang, Z. W., Zhao, X., Zhong, W. Y., Chen, H., Yin W. L., Huang, T., Niu, S. C. and Liu, Z. J. (2021). Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchids evolution. Horticulture Research, 8:183.
Zheng, S. G., Hu, Y. D., Zhao, R. X., Yan, S., Zhang, X. Q., Zhao, T. M. and Chun, Z. (2018). Genome-wide research and applications on Dendrobium. Planta, 248:769-784.