Isolation, identification of 16S rRNA gene sequences analysis and co-inoculation of Pseudomonas fluorescens (LNPF1) and Bacillus subtilis (LSBS2) for Plant Growth Promotion and Nutrient Fortification in Arachis hypogaea
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Abstract
In the Salem area of Tamil Nadu, India, rhizospheres of agricultural soil were found to contain rhizobacteria that promote plant development. These isolates were recognised as Pseudomonas fluorescens (LNPF1) and Bacillus subtilis (LSBS2) based on their biochemical traits and 16S rRNA gene sequence analysis. The PGP activity, phosphate solubilisation, IAA production, HCN production, ammonium production, and siderophore production of both isolates were examined. Furthermore, in pot experiment was conducted with Arachis hypogaea resulted to be the highest yield parameter in co-inoculation of LNPF1+LSBS2 significantly increased in shoot length 39.16±0.37, root length 31.13±0.55, fresh weight 12.5±0.30 dry weight 7.03±0.15, total nitrogen 19.33±0.64 mg N/g , chlorophyll 0.479±0.58 mg/g and carotenoids content 0.573±0.01 mg/g in treated plantsd. Biomass accumulation of root nodules were observed the highest number in LNPF1+LSBS2 53.00±1.00 nodules fresh weight, and dry weight also increased. Nitrogenase activity was more in LNPF1+LSBS2 17.42±1.15 n moles. Leghemoglobin assay with inoculation of LNPF1+LSBS2 1.70mg/g was comparable to those un-inoculated plants of 1.16mg/g. Nutrients content also increased in protein of 15.00±0.13%, carbohydrate 24.75±0.98%, fat 47.69±0.15%, fiber 15.40±0.31%, iron 7.36±0.5mg/g, calcium 16.63±0.20mg/g, and energy 563Kcal when compared to that uninoculated plants. Total seed oil content, bioinoculant-treated plants with 50.06±0.42% un-inoculated plants which found to be 34.93±0.56 % of oil content. Therefore, the strain LNPF1 (Pseudomonas) and LSBS2 (Bacillus) could be exploited as a potential bioinoculant for plant growth and increased in plant nutrients, oil yielding content, and increased in iron content of Arachis hypogaea.
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