Application of antagonistic fungi to control anthracnose disease of grape
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Abstract
Anthracnose of the grape varieties Bigblack, Nanpha, Blackopal, Loose perlette and White malaca are caused by Colletotrichum gloeosporioides. All isolates obtained from grape anthracnose were shown to be pathogenic; isolate WMF01 was the most virulent on all tested varieties of grape. Assays using crude extracts from Chaetomium cupreum CC, C. globosum CG, Trichoderma harzianum PC01, T. hamatum PC02, Penicillium chrysogenum KMITL44 and antibiotic substances Rotiorinol, Chaetoglobosin-C and Trichotoxin A50 were carried out to test bioactivity. All extracts and compounds inhibited the growth of C. gloeosporioides strain WMF01, with average ED50 values between 1 to 50 ppm. Applications of bioproducts of Chaetomium, Penicillium and Trichoderma, and a mixture of those bioproducts in a powder formulation and a chemical control were conducted in the field to control anthracnose disease of 5-varieties of grape. All bioproducts significantly reduced the disease incidence on leaves, twigs and fruits of grape in all varieties as compared to the chemical control.
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