Identification of new position of DNA insertion and 24-bp INDEL mutation polymorphism in prolactin gene promoter of Thai native chickens

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Published: Jul 15, 2025
DOI: https://doi.org/10.63369/ijat.2025.21.4.1555-1562
Keywords:
Prolactin promoter INDEL mutation 24 bp polymorphism DNA duplication Chicken

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Thongsaiklaing, T.
Faculty of Agriculture, Princess of Naradhiwas University, Narathiwas, 96000, Thailand, 2Biotechnology and Molecular Biology Laboratory (BMBL), Faculty of Agriculture, Princess of Naradhiwas University, Narathiwat, 96000, Thailand
Datumada, H.
Faculty of Agriculture, Princess of Naradhiwas University, Narathiwas, 96000, Thailand, Biotechnology and Molecular Biology Laboratory (BMBL), Faculty of Agriculture, Princess of Naradhiwas University, Narathiwat, 96000, Thailand
Morris, J.
Faculty of Engineering, Mahasarakham University, Mahasarakham, 44150, Thailand
Passara, H.
School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand

Abstract

This research identified a 24-bp INDEL (-358) on the promoter region of the PRL gene in Thai native chickens. The allele frequencies for allele I and allele D were 0.20 and 0.80, respectively. The genotype frequencies were II (0.028), ID (0.349), and DD (0.622), respectively. The heterozygosity values comprised the Ho (0.350), higher than the He value (0.323). Moreover, our findings revealed new DNA duplication on the PRL promoter region, with three distinct polymorphisms of 30 bp (-358), 24 bp (-358), and 14 bp (-358), respectively.

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How to Cite
Thongsaiklaing, T., Datumada, H., Morris, J., & Passara, H. (2025). Identification of new position of DNA insertion and 24-bp INDEL mutation polymorphism in prolactin gene promoter of Thai native chickens. International Journal of Agricultural Technology, 21(4), 1555–1562. https://doi.org/10.63369/ijat.2025.21.4.1555-1562
Issue
Vol. 21 No. 4 (2025): July
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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