Effects of Several Organic Extracts on the Growth, Yield and Quality of Anoectochilus Formosanus Biomass
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Abstract
Anoectochilus formosanus is a high economic value herbal remedy, the oldest in herbal medicine. Nowadays, A. formosanus has been applied in many products of caring human health. However, A. formosanus in nature have slow growth characteristics, restrictive adapting conditions and susceptible to fungal diseases. Application of plant tissue culture technology can help to produce a large amount of A. formosanus biomass in controllable conditions. To be able to use A. formosanus biomass for serving human, plant growth regulators should not be used because of their disadvantageous affected on production quality. This topic surveys the effects of some organic extracts, using traditional plant tissue culture, on growing ability, yield and quality of A. formosanusbiomass. Some residual minerals which are useless for our health, such as NO3-, heavy metals (Cu, Zn, etc.) were also investigated for evaluating the quality of A. formosanusbiomass. After 60 days of culture, media supplementing with coconut water, yeast extract or Spirulina extract separately influenced positively to the growth and development of in-vitro A. formosanus. Yeast extract with the concentration of 2 g/l obtained the highest results in all targets, average height of explant was 11.69 cm/explant, average fresh weight was 2.57 g/explant. Contents of NO3-, Cu2+, Zn2+ are lower than the allowed threshold of FAO in all treatments. This study also examines the influence of culture density effecting on the yield capacity of A. formosanus. Results showed that, when cultured at a density of 10 explants per sigma box, their growth and development were better than others, achieved 251.44 g/L (total biomass/medium)
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