Improved developmental competence of swamp buffalo oocytes matured in the presence of Cysteamine

Article Sidebar

PDF
Published: Jan 16, 2015
Keywords:
Cysteamine Embryos Glutathione Maturation Pronuclei

Main Article Content

Ocampo, L. C.
Reproductive Biotechnology Unit, Philippine CarabaoCenter, Science City of Munoz, Nueva Ecija, Philippines
Ocampo, M. B.
Reproductive Biotechnology Unit, Philippine CarabaoCenter, Science City of Munoz, Nueva Ecija, Philippines

Abstract

The study examined the effect of cysteamine addition on the acquisition of swamp buffalo oocytes developmental competence in vitro and on the intracytoplasmicglutathione level. In a dose dependent manner, results showed significant improvement on the maturation, penetration and MPN formation of oocytes cultured with 50 µM cysteamine. Also, improved cleavage and development to the blastocyst stage were observed in oocytes matured with cysteamine addition (23.0%) versus the control (11.1%). Similarly, the intracytoplasmic glutathione level improved in oocytes cultured with cysteamine versus the control though remained lower compared to in vivo matured oocytes. Overall, the results suggest that an increase in the intracellular glutathione level of swamp buffalo oocytes as induced by cysteamine during maturation provided the oocytes with a favorable intracellular condition to support maturation, fertilization and the subsequent embryonic development in vitro.

Article Details

How to Cite
Ocampo, L. C., & Ocampo, M. B. (2015). Improved developmental competence of swamp buffalo oocytes matured in the presence of Cysteamine. International Journal of Agricultural Technology, 11(1), 31–40. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/5987
Issue
Vol. 11 No. 1 (2015): January
Section
Original Study
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Berger, T. (1990). Changes in exposed molecular protein during in vitro capacitation of boar sperm. Molecular reproduction and development 27:249-253.

De Matos, D. G., Furus, C. C., Moses, D. F. and Baldasare, H. (1995). Effect of cysteamine on GSH level and developmental capacity of bovine oocytes matured in vitro. Molecular Reproduction and Development 42:432-436.

De Matos D. G., Furnus C. C., Moses, D. F., Martinez, D. G. and Matccovic, D. (1996). Stimulation of GSH synthesis of in vitro matured bovine oocytes and its effect on embryo development and freezability. Molecular Reproduction and Development 45:451-457.

De Matos, D. G., Furnus, C. C. and Moses, D. F. (1997). GSH synthesis during in vitro maturation of bovine oocytes: role of cumulus cells. Biology of reproduction 57:1420-1425.

Eppig, J. (1996). Coordination of nuclear and cytoplasmic oocyte maturation in eutherian mammals. Reproduction, Fertility and Development 8:485-489.

Funahashi, H., Cantley, T. C., Stump, T. T., Terlouw, S. L. and Day, B. N. (1994). Use of low salt culture medium for in vitro maturation of porcine oocytes is associated with elevated oocyte GSH level and enhanced MPN formation after in vitro fertilization. Biology of Reproduction 51:633-639.

Grupen, C. G., Nagashimam, H. and Nottle, M. B. (1995). Cysteamine enhances in vitro development of porcine oocytes matured and fertilized in vitro. Biology of Reproduction 53:173-178.

Ocampo, L. C., Cruz, L. C. and Ocampo, M. B. (1997). Some factors influencing the maturation, fertilization and development of buffalo oocytes in vitro. Proceeding of the 25th Veterinary Practitioners Association of the Philipines, Manila, Philipines. pp. 75-81.

Perreault, S. D., Wolf, R. A. and Zirkin, B. R. (1984). Role of disulfide bond reduction during mammalian sperm nuclear decondensation in vivo. Developmental Biology 101:160-167.

Perreault, S. D., Barbee, R. R. and Slott, V. I. (1988). Importance of GSH in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes Developmental Biology 125:181-186.

Perreault, S. D. (1990). Regulation of sperm nuclear reactivation during fertilization. In Bavister, B. D., Cummins, J. and Roldan, E. R. S. (Eds), Fertilization in Mammals. Norwell: Serono Symposia. pp. 285-296.

Rose, T. A. and Bavister, B. D. (1992). Effect of oocyte maturation medium on in vitro development of in vitro fertilized bovine oocytes. Molecular Reproduction and Development 31:72-77.

Sawai, K. H., Funahashi, W., Wang, H. and Niwa, K. (1997). Effects of cysteine in serum-free maturation medium on MPN formation of maturing pig oocytes penetrated in vitro. Journal of Reproduction and Development 43:73-80.

Sutovsky, P. and Schatten, G. (1997). Depletion of GSH during bovine oocyte maturation reversibly blocks the decondensation of the MPN and pronuclear apposition during fertilization. Biology of Reproduction 56:1503-1512.

Suizuki, T., Singla, S. K., Sujata, S. and Madan, M. L. (1992). IVF of water buffalo follicular oocytes and their ability to cleave in vitro. Theriogenology 38:1187-1194.

Thouas, G. A., Korfiatis, N. A., French, A. J., Jones, G. M. and Ttounson, A. O. (2001). Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts. Reproduction BioMedicine Online 3:25-29.

Takahashi, M., Nagai, T., Hamano, S., Kuwayama, M., Okamura, N. and Okano, A. (1993). Effect of thiol compounds on in vitro development and intracellular GSH content of bovine embryos. Biology of Reproduction 49:228-232.

Wiesel, S. and Schultz, G. A. (1981). Factors which may affect removal of protamine from sperm DNA during fertilization in rabbit. Gamete Research 4:25-34.

Yoshida, M., Ishigaki, K. and Pursel, V. G. (1992). Effect of maturation media on MPN formation in pig oocytes matured in vitro. Molecular Reproduction and Development 31:68-71.

Yoshida, M., Ishigaki, K., Nagai, T., Chikyu, M. and Pursel, V. G. (1993). GSH concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form MPN. Biology of Reproduction 49:89-94.

Zirkin, B. R., Soucek, D. A., Chang, T. S. K. and Perreault, S. D. (1985). In vitro and in vivo studies of mammalian sperm nuclear decondensation. Gamete Research 11:349-365.