Association of FABP3 and LEPR gene polymorphisms with the drip loss trait of pork
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Abstract
Drip loss is a major parameter for the quality of pork which affects the economic perspective of premium-meat in the world’s pork industry. It is clear that the fatty acid binding protein3 (FABP3) gene is related to oxidation and glucose utilization in muscles whereas the leptin receptors (LEPR) gene is related to energy balance, and both genes affect pork quality. The objective of this study was to analyze the genetic polymorphisms of FABP3 and LEPR genes associated with the drip loss trait of pork. Longissimus dorsi muscle samples were taken from a total of 1,114 commercial pigs including purebred Duroc and [(Duroc × Large White) × Landrace] × Duroc. DNA was extracted by the Chelex® method. Drip loss was measured by the bag method based on gravitational technique. The FABP3 and LEPR genes were genotyped by the PCR-RFLP technique. It was found that the FABP3 gene showed significant association with the drip loss trait. The genotypes GC and CC of [(Duroc × Large White) × Landrace] × Duroc had the lowest drip loss. The LEPR gene was also associated with the drip loss trait. The animals of genotype TT had the lowest drip loss in Duroc but the genotype AA had the lowest drip loss in [(Duroc × Large White) × Landrace] × Duroc. Furthermore, the interaction between FABP3 and LEPR significantly affected drip loss. The animals with genotypes GGTT, GCTT and CCTT had the lowest drip loss in Duroc whereas the animals with genotypes GCAA, CCAA, GGAA, CCTT, GCTT, and CCTA had the lowest drip loss in [(Duroc × Large White) × Landrace] × Duroc. These results indicated the importance of FABP3 and LEPR genes to be used for the marker-assisted selection for the improvement of pork quality.
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