Effects of accelerated aging and subsequent priming on seed quality and biochemical change of hybrid cucumber (Cucumis sativa Linn.) seeds

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Published: Jan 16, 2015
Keywords:
Seed priming Seed enhancements Seed germination Antioxidatants Cucumber

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Krainart, C.
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Siri, B.
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Vichitphan, K.
Department of Biotechnology and Fermentation Research Center for Value added Agriculture Products (FerVAAP), Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Abstract

The research was aimed to study beneficial effects of seed priming treatment on various deteriorated seeds under artificial accelerated aging. It was focused on seed quality and biochemical parameters which were germination percentage, speed of germination, and mean germination time, lipid peroxidation (malondialdehyde and total peroxide), and antioxidant activity (ascorbate, dehydroascorbate, catalase and %DPPH inhibition). Experiments were tested for seed priming and accelerated aging which conducted in the seed processing plant with the hybrid cucumber seeds of cultivar SPP012. Seeds were brought to incubate aging chamber to obtain 45°C
and relative humidity of 100 % for 3, 6, 9, 12, 15, 18 and 21 days. Subsequently, all of the seeds were dried at 35 °C for 48 hours by the modified air-dryer to reduce seed moisture to 7-8%. Aged seeds were soaked in KH2PO4 and using PEG 6000 as the agent to adjust a potential to be -1 MPa at 15°C for 72 hours. Primed seeds were rinsed with deionized water and adjusted to the initial seed moisture by air-drying at 35°C for 48 hours. The samples were randomly selected to examine the effects of accelerated aging to monitor seed quality in the laboratory and greenhouse. Germination tests were performed to examine seeds quality. Biochemical analysis covered lipid peroxidation and antioxidant activity. The results found that seed priming was improved the quality of hybrid cucumber seeds and biochemical parameters at significant level. Level of germination defines effectiveness of seed priming in commercial purpose. Seed priming significantly reduced amount of malondialdehyde and total peroxide.   The antioxidant activity, seed priming affects various results of biochemical changes were expressed by increasing the level of antioxidant activity.

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How to Cite
Krainart, C., Siri, B., & Vichitphan, K. (2015). Effects of accelerated aging and subsequent priming on seed quality and biochemical change of hybrid cucumber (Cucumis sativa Linn.) seeds. International Journal of Agricultural Technology, 11(1), 165–179. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6010
Issue
Vol. 11 No. 1 (2015): January
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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