Isolation and identification of bacterial wilt causing Ralstonia solanacearum from groundnut (Arachis hypogaea L.)
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Abstract
Bacterial wilt caused by Ralstonia solanacearum is one of the production constraints of groundnut. The wilt incidence, distribution, losses, symptoms and their epidemiology were investigated as well as the field survey to evaluate the severity and disease incidence in the regions of Andhra Pradesh and Karnataka. Result showed that 80 isolates were derived from wilted samples, and identified by the biochemical, pathogenicity and molecular characteristics. Among 80 isolates, sixty five isolates were categorized as Race 2 biovar III and the remaining 15 isolates belonged to biovars I and V based of sugars utilization. Pathogenicity assay suggested that out of 80 isolates, 25 isolates were highly pathogenic, 30 isolates showed 70-80% wilt incidence, 16 isolates showed 50% wilt, and 8 isolates did not cause any wilt symptoms. R. solanacearum is confirmed identification by molecular analysis
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