Efficacy of New Herbal Shampoos from Garcinia dulcis Kurz, Citrus aurantium L. and Eucalyptus globulus Labill as Pediculicides for Head Lice (Pediculus humans capitis) Control

Article Sidebar

PDF
Published: Jul 15, 2018
Keywords:
Pediculus humanus capitis Herbal shampoo Garcinia dulcis Citrus aurantium EO Eucalyptus globulus EO

Main Article Content

Sittichok, S.
Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalong Krung Road, Ladkrabang, Bangkok, Thailand
Soonwera, M.
Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalong Krung Road, Ladkrabang, Bangkok, Thailand

Abstract

Currently, synthetic chemical pediculicides have lost their efficacy due to worldwide increased resistance of head lice to them. Therefore, safe, natural product alternatives are in dire need. This study investigated the efficacy of an herbal shampoo made from Garcinia dulcis (Roxb.) added with either Citrus aurantium EO or Eucalyptus globulus EO against head lice in both in vitro and in vivo tests. In vitro experiment used a filter paper contact method to evaluate the pediculicidal activity of the shampoo at 0.002, 0.003 and 0.006 ml/cm2 doses (per unit area of petri dish plate) on nymphs and adults of head lice. In vivo trial, the infested children were treated with the shampoo. The results showed either cured or not cured of pediculosis. The main results showed LC50 values against nymphs for G. dulcis + C. aurantium EO shampoo and G. dulcis + E. globulus EO shampoo were 0.00001 and 0.00004 ml/cm2, respectively. Those actively against the adults which were 0.7 and 0.9 ml/cm2, respectively. In vivo test revealed that G. dulcis + C. aurantium EO shampoo and G. dulcis + E. globulus EO shampoo were the most effective pediculicide, showing 100% cure rate after the 2nd application, much more effective than the tested chemical pediculicide. It concluded that these shampoos can be highly affected pediculicide alternatives and safe for treating humans.

Article Details

How to Cite
Sittichok, S., & Soonwera, M. (2018). Efficacy of New Herbal Shampoos from Garcinia dulcis Kurz, Citrus aurantium L. and Eucalyptus globulus Labill as Pediculicides for Head Lice (Pediculus humans capitis) Control. International Journal of Agricultural Technology, 14(4), 597–612. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6044
Issue
Vol. 14 No. 4 (2018): July
Section
Original Study
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Abdel-Ghaffar, F., Abdel-Aty, M., Rizk, I., Al-Quraishy, S., Semmler, M., Gestmann, F. and Hoff, N. P. (2016). Head lice in progress: what could/should be done-a report on an in vivo and in vitro field study. Parasitology Research 115:4245-4249.

Abu Bakar, M. F., Ahmad, N. E., Suleiman, M., Rahmat, A. and Isha, A. (2015). Garcinia dulcis fruit extract induced cytotoxicity and apoptosis in HepG2 liver cancer cell line. BioMed Research International 2015:916902. doi: 10.1155/2015/916902.

Allen, H. and Cox, J. (2018). Permethrin cream (Lyclear). 20 March 2018. Retrieved from http://patient.info/medicine/permethrin-cream-lyclear.

American Academy of Pediatrics. (2017). Head lice: what parents need to know. 20 December 2017. Retrived from https://www.headltychildren.org.

Audino, P. G., Vassena, C., Zerba, E. and Picollo, M. (2007). Effectiveness of lotions based on essential oils from aromatic plants against permethrin resistant Pediculus humunus capitis. Archives of Dermatological Research 299:389-392.

Badawy, M. E. I., Taktak, N. E. M. and El-Aswad, A. F. (2017). Chemical composition of the essential oils isolated from peel of three citrus species and their mosquitocidal activity against Culex pipiens. Natural Product Research 10:1-6.

Barbosa, L. C., Filomeno, C. A. and Teixeira, R. R. (2016). Chemical variability and biological activities of Eucalyptus spp. essential oils. Molecules 21:E1671.

Beier, R. C., Byrd, J. A., Kubena, L. F., Hume, M. E., McReynolds, J. L., Anderson, R. C. and Nisbet, D. J. (2014). Evaluation of linalool, a natural antimicrobial and insecticidal essential oil from basil: effects on poultry. Poultry Science 93:267-272.

Bowles, V. M., Yoon, K. S., Barker, S. C., Tran, C., Rhodes, C. and Clark, M. J. (2017). Ovicidal efficacy of abametapir against eggs of human head and body lice (Anoplura: Pediculidae). Journal of Medical Entomology 54:167-172.

Candy, K., Nicolas, P., Andriantsoanirina, V., Izri, A. and Durand, R. (2018). In vitro efficacy of five essential oils against Pediculus humanus capitis. Parasitology Research 117:603-609.

Centers for Disease Control and Prevention (2017). Head lice treatment. 25 December 2017. Retrived from http://www.cdc.gov/dodx/contact.html.

Cueto, G. M., Zerba, E. N. and Picollo, M. I. (2008). Evidence of pyrethroid resistance in eggs of Pediculus humunus capitis (Phthiraptera: Pediculidae) from Argentina.Journal of Medical Entomology 45:693-697.

Dambolena, J. S., Zunino, M. P., Herrera, J. M., Pizzolitto, R. P., Areco, V. A. and Zygadlo, J. A. (2016). Terpenes: natural products for controlling insects of importance to human health-a structure- activity relationship study. Psyche. A Journal of Entomology 2016:4595823. http://dx.doi.org/10.1155/2016/4595823.

Deachathai, S., Mahabusarakam, W., Phongpaichit, S. and Taylor, W. C. (2005). Phenolic compounds from the fruit of Garcinia dulcis. Phytochemistry 66:2368-2375.

Devore, C. D. and Schutze, G. E. (2015). Council on school health and committee on infectious diseases. Journal of Pediatrics 135:e1355-65.

Di Campli, E., Di Bartolomeo, S., Delli Pizzi, P., Di Giulio, M., Grande, R., Nostro, A. and Cellini, L. (2012). Activity of tea tree oil and nerolidol alone or in combination against Pediculus capitis (head lice) and its eggs. Parasitology Research 111:1985-1992.

Doroodgar, A., Sadr, F., Doroodgar, M., Doroodgar, M. and Sayyah, M. (2014). Examining the prevalence rate of Pediculus capitis infestation according to sex and social factors in primary school children. Asian Pacific Journal of Tropical Disease 4:25–29.

Durand, R., Bouvresse, S., Berdjane, Z., Izri, A., Chosidow, O. and Clark, J. M. (2012). Insecticide resistance in head lice:clinical, parasitological and genetic aspects.Clinical Microbiology and Infection 18:338-344.

Eisenhower, C. and Farrington, E. A. (2012). Advancements in the treatment of head lice in pediatrics. Journal of Pediatric Health Care 26:451-461.

Eroglu, F., Basaran, U., Kurklu, C. G., Yuceer, M., Yalcinturk, R. G., Tanriverdi, M., Dagli, E. I. and Koltas, I. S. (2016). Pediculosis capitis is a growing neglected infestation due to migration in Southeast Turkey. Parasitology Research 115:2397-2401.

Gallardo, A., Picollo, M. I., González-Audino, P. and Mougabure-Cueto, G. (2012). Insecticidal activity of individual and mixed monoterpenoids of geranium essential oil against Pediculus humanus capitis (Phthiraptera: Pediculidae). Journal of Medical Entomology 49:332-335.

Hutadilok-Towatana, N., Kongkachuay, S. and Mahabusarakam, W. (2007). Inhibition of human lipoprotein oxidation by morelloflavone and camboginol from Garcinia dulcis. Natural Product Research 21:655-662.

Ko, C. J. and Elston, D. M. (2004). Pediculosis. Journal of the American Academy of Dermatology 50:1-12.

Lamai, J., Mahabusarakam, W., Ratithammatorn, T. and Hiranyachattada, S. (2013). Effects of morelloflavone from Garcinia dulcis on vasorelaxation of isolated rat thoracic aorta. Journal of Physiological and Biomedical Sciences 26:13-17.

Leung, A. K. C., Fong, J. H. S. and Pinto-Rojas, A. (2005). Pediculosis capitis. Journal of Pediatric Health Care 19:369-373.

Lim, T. K. (2011). Edible medicinal and non-medicinal plants. Fruits 2:35-40.

National Association of School Nurses. (2018). Head lice management in the school setting. 20 January 2018. Retrived from https://schoolnursenet.nasn.org.

Sanei-Dehkordi, A., Sedaghat, M. M., Vatandoost, H. and Abai, M. R. (2016). Chemical compositions of the peel essential oil of Citrus aurantium and its natural larvicidal activity against the malaria vector Anopheles stephensi (Diptera: Culicidae) in comparison with Citrus paradisi. Journal of Arthropod-Borne Diseases 10:577–585.

Sinthusart, U. (2015). Herbs of Chao-Krom-Poe Dispensary. 5th ed. Bangkok: Thailand. pp. 356.

Strycharz, J. P., Lao, A. R., Alves, A. M. and Clark, J. M. (2014). Ovicidal response of NYDA formulations on the human head louse (Anoplura: Pediculidae) using a hair tuft bioassay. Journal of Medical Entomology 49:336-342.

Suryawanshi, J. A. S. (2011). An overview of Citrus aurantium used in treatment of various diseases. African Journal of Plant Science 5:390-395.

Toloza, A. C., Zygadlo, J., Cueto, G. M., Biurrun, F., Zerba, E. and Picollo, M. I. (2006). Fumigant and repellent properties of essential oils and component compounds against permethrin resistant Pediculus humunus capitis (Anoplura: Pediculidae) from Argentina. Journal of Medical Entomology 43:889-895.

Toloza, A. C., Lucía, A., Zerba, E., Masuh, H. and Picollo, M. I. (2010). Eucalyptus essential oil toxicity against permethrin-resistant Pediculus humanus capitis (Phthiraptera: Pediculidae). Parasitology Research 106:409-414.

Tracy, T. S. and Kingston, R. L. (2007). Herbal products: toxicology and clinical pharmacology. Totowa: New Jersey. pp. 288.

Tuansulong, K. A., Hutadilok-Towatana, N., Mahabusarakam, W., Pinkaew, D. and Fujise, K. (2011). Morelloflavone from Garcinia dulcis as a novel biflavonoid inhibitor of HMG-CoA reductase. Phytotherapy Research 25:424-428.

Wadowski, L., Balasuriya, L., Price, H.N. and O'Haver, J. (2015). Lice update: new solutions to an old problem. Clinics in Dermatology 33:347-354.

Watcharawit, R. and Soonwera, M. (2013). Pediculicidal effect of herbal shampoo against Pediculus humunus capitis in vitro. Tropical Biomedicine 30:315-324.

West, D. P. (2004). Head lice treatment costs and the impact on managed care. The American Journal of Managed Care 10:S277-82.