Molecular identification of Anaplasma ovis and Anaplasma marginale in Sheep using PCR-RFLP method
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Abstract
Anaplasma is a protozoan blood in livestock such as cattle, goats and sheep. This pathogen leads to anaplasmosis, which is tick-borne disease, production loss and high costs due to disease prevention. Therefore, it is necessary to investigate the epidemiology of Anaplasma of livestock. The results demonstrated that the semi-nested-PCR product was approximately 120 bp. The restriction endonuclease Bst1107I only recognized the sequence (GTATAC) in semi-nested PCR product of A. marginale and cut the sequence at the position 68. However, the restriction endonuclease was not able to cut the semi-nested PCR product of A. ovis at the same position because of the different sequence (GTACGC). The prevalence of A. ovis and A. marginale infection in sheep at Sa Kaeo Province was 3.66% (3/82) and 1.22% (1/82), respectively. The overall prevalence of Anaplasma infection in sheep was 7.32% (6/82). From this result, the PCR-RFLP method could be used to isolate A. ovis and A. marginale using this technique with high specificity and safe time to identify both types of infection
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