In vitro regeneration from callus and cell suspension cultures in Indian mustard [Brassica juncea (Linn.) Czern & Coss]

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Published: May 15, 2021
Keywords:
Indian mustard Callus Cell suspension culture Somatic embryogenesis Plantlet regeneration

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Shyam, C.
Department of Genetics & Plant Breeding, College of Agriculture, RVS, Agricultural University, Gwalior, 474002 M.P. India
Tripathi, M. K.
Department of Genetics & Plant Breeding, College of Agriculture, RVS, Agricultural University, Gwalior, 474002 M.P. India; Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India
Tiwari, S.
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India
Ahuja, A.
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India
Tripathi, N.
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India
Gupta, N.
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India

Abstract

Brassica juncea  is a crucial mustard species cultivated in India for edible oil. An efficient and reproducible plant regeneration protocol is established from callus using mature cotyledons and seeds as an explants and embryogenic cell suspension cultures dervied from embryogenic fraible calli in present investigation. The best callus induction was obtained with MS basal media fortified with 3.0 mgl-1 2, 4-D. Further regeneration via embryogenesis/ organogenesis was attained with supplementation of 0.5 mgl-1  BAP in combination with 0.5-1.0 mgl-12,4-D. The superiority of mature seeds as explants was documented clearly points towards that this explant had higher morphogenic potential. Further, effectiveness of regeneration via callus was quite significantly correlated with genotype, type (s) and relative concentrations and combinations of plant growth regulators. These protocols may be employed in genetic transformation and in vitro selection purposes in future of elite genotype of Indian mustard.

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How to Cite
Shyam, C., Tripathi, M. K., Tiwari, S., Ahuja, A., Tripathi, N., & Gupta, N. (2021). In vitro regeneration from callus and cell suspension cultures in Indian mustard [Brassica juncea (Linn.) Czern & Coss]. International Journal of Agricultural Technology, 17(3), 1095–1112. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6100
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Vol. 17 No. 3 (2021): May
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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