Efficiency of Antioxidant and Absorbent on Browning and The Optimal Factors of Plant Regeneration from Young Seed of Gluta usitata (217 Mae Ka) by Tissue Culture
Article Sidebar
Main Article Content
Abstract
Gluta usitata (Anacardiaceae) is a lacquer tree and create lacquer varnish can be utilized in many aspects especially art and culture. Recently, its harvest faced troublesome and yields of varnish tree are decreased. Consequently, the conservation of plant has reduced, and this plant has become extinct. The experiment was chosen to use plant tissue culture technique to preserve and increase this plant. The experiment was compared the effect of Woody Plant Medium (WPM) combined with different concentration of antioxidants were 2, 4, 6, 8 g/L Poly Vinyl Pyrrolidone (PVP), 20, 30, 40, 60 mg/L ascorbic acid or citric acid and absorbents of 1, 2, 3 g/L activated charcoal to reduce browning of phenolic compound in the explants. The results showed that 2 g/L activated charcoal gave the lowest of browning on seed and medium (about 0.9±0.2 points). The addition of 40 mg/L ascorbic acid was generated the highest of shoots (approximately 1.88±0.4 shoots/seed) after culture for 4 weeks. in vitro propagation of seed germination on WPM supplemented with 0.5, 1, 1.5, 2 and 3 mg/L 6-Benzyl amino purine (BAP), Thidiazuron (TDZ) and meta-Topolin (mT) 3% sucrose and 0.2% phytagel was efficiently resulted. Other result revealed that 1.5 mg/L BAP, 2 mg/L TDZ and 2 mg/L mT gave the highest seed germination at 1.6±0.2, 1.8±0.2 and 1.6±0.2 shoots/seed, respectively. The best of plant growth regulators for seed germination was 2 mg/L of TDZ after 6 weeks.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Aliyu, O. M. (2005). Application of tissue culture to cashew (Anacardium occidentale L.) breeding: An appraisal. African Journal of Biotechnology. 4:1485-1489.
Benmahioul, B., Dorion, N., Harche, M. K. and Daguin, F. (2012). Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.). Plant Cell, Tissue and Organ Culture. 108: 353-358.
Benmahioul, B., Harche, M. K. and Daguin, F. (2016). In vitro regeneration of Pistacia vera L. from nodal explants. Journal of Forest Science. 62:198-203.
Benmahioul, B., Kaid-Harche, M., Dorion, N. and Daguin, F. (2009). In vitro embryo germination and proliferation of pistachio (Pistacia vera L.). Scientia Horticulturae. 122:479-483.
Boggetti, B., Jasik, J. and Mantell, S. (1999). In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plants. Plant Cell Reports. 18:456-461.
Boggetti, B., Jasik, J. and Mantell, S.H. (2001). In vitro root formation in Anacardium occidentale microshoots. Biologia Plantarum. 44:175-179.
Boonyaprapatsorn, N. and Chokchaicharoenporn, O. (2542). Traditional herb. Bangkok, Thailand.
Dunuwila, D. M. P. M. K., Kottearachchi, N. S., Dassanayaka, M. P., Attanayaka, D. P. S. T. G. and Jayasekara, S. J. B. A. (2010). In Vitro Shoot Multiplication and Rooting of Cashew (Anacardium Occidentale L.). Proceedings of 10th Agricultural Research Symposium. 6-10.
Eng, W. H., Aziz, M. A. and Sinniah, U. R. (2015). In vitro regeneration of Citrus hystrix DC. Brazilian Journal of Botany. 38:235-242.
Fine Arts Department. (2008). Seminar on study of oriental lacquer initiated by H.R.H.Princess Maha Chakri Sirindhorn for the revitalization of Thai wisdom. [Online] Available: http://www.changsipmu.com/book-rak-tree-contents.html.
Gannoun, S. I., Lionakis, S. M. and Gerasopoulos, D. (1995). Aspects of In vitro culture of Pistacia terebinthus and Pistacia vera. Acta Horticulturae. 419:201-206.
George, E. F. (1993). Plant Propagation by Tissue Culture. Part I. The Technology. 2nd Ed. Edington, Exegetics:574.
Huetteman, C. A. and Preece, J. E. (1993). Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tissue and Organ Culture. 33:105-119.
Kidwai, N. R., Jain, M. B. and Chaturvedi, H. C. (2009). Role of thidiazuron in In vitro induction of embryogenesis in nucellar tissue of Mangifera indica L. var. Dashehari, leading to plantlets. Current Science. 96:1119-1124.
Kittibanpacha, K., Vacharangkuura, T. and Jetapai, C. (2015). Study on Gluta usitata (Wall.) Ding Hou vegetative propagation technique. [Online] Available: http://forprod.forest.go.th/forprod/Project_research57/PDF/5.pdf.
Kozgar, M. I. and Shahzad, A. (2012). An improved protocol for micropropagationof teak tree (Tectona grandis L.). Rendiconti Lincei. 23:195-202.
Krishna, H., Sairam, R. K., Singh, S. K., Patel, V. B., Sharma, R. R., Grover, M., Nain, L. and Sachdev, A. (2008). Mango explants browning: effect of ontogenic age, mycorrhization and pretreatments. Scientia Horticulturae. 118:132-138.
Moyo, M., Finnie, J. F. and Van, S. J. (2012). Microculture effects on leaf epidermis and root structure in Sclerocarya birrea subsp. caffra. South African Journal of Botany. 78:170-178.
Murthy, B. N. S. and Praveen, K. S. (1998). Somatic embryogenesis and plant regeneration of neem (Azadirachta indica A. Juss.). Plant Cell Reports. 17: 469-475.
Onay, A., Jeffree, C. E. and Yeoman, M. M. (1996). Plant regeneration from encapsulated embryoids and an embryogenic mass of pistachio (Pistacia vera L.). Plant Cell Reports. 15:723-726.
Onay, A., Pirinç, V., Yildirim, H. and Basaran, D. (2004). In vitro micrografting of mature pistachio (Pistacia vera var. Siirt). Plant Cell, Tissue and Organ Culture. 77:215-219.
Panda, B.M. and Hazra, S. (2009). Seedling culture of Semecarpus anacardium L. Seed Science and Biotechnology. 3:54-59.
Panda, B.M. and Hazra, S. (2010). In vitro regeneration of Semecarpus anacardium L. from axenic seedling-derived nodal explants. Trees. 24:733-742.
Pianhanuruk, C. (2007). Tissue culture techniques for teak young seed. Forest research database.
Raghuvanshi, S. S. and Srivastava, A. (1995). Plant regeneration of Mangifera indica using liquid shaker culture to reduce phenolic exudation. Plant Cell, Tissue and Organ Culture. 41:83-85.
Sansberro, P., Rey, H. and Mroginski, L. (2003). In vitro plantlet regeneration of Schinopsis balansae (Anacardiaceae). Trees. 17:542-546.
Sija, S. L., Potty, V. P. and Santhoshlal, P. S. (2015). Impact of culture media and growth hormones on callus induction and root organogenesis in cotyledon segment of Anacardium occidentale L. Annals of Plant Sciences. 4:1057-1063.
Tabthong, O. (2012). 84 trees species for King. [Online] Available: libapp.tsu.ac.th/ULIB/dublin.php? ID=979.
Tangmitcharoen, S., Eiadthong, W., Vacharangkura, T., Kittibanpacha, K. and Yongratana, P. Gene conservation and tree improvement of Lacquer Trees in Thailand. [Online] Available:http://www.conference.forest.ku.ac.th/iDocument/edit_20150407_140737.pdf.
Thimmappaiah, S. R. A., Puthra, G. T. and Shirly, R. A. (2002a). In vitro grafting of cashew (Anacardium occidentale L.). Scientia Horticulturae. 92:177-182.
Thimmappaiah, S. R. A., Shirly, R. A. and Sadhana, P. H. (2002b). In vitro propagation of cashew from young trees. In Vitro Cellular & Developmental Biology – Plant. 38:152-156.
Tilkat, E. and Onay, A. (2009). Direct shoot organogenesis from In vitro derived mature leaf explants of pistachio. In Vitro Cellular & Developmental Biology – Plant. 45:92-98.
Tilkat, E., Isikalan, C., and Onay, A. (2005). In vitro propagation of khinjuk pistachio (Pistacia khinjuk Stocks) through seedling apical shoot tip culture. Propagation of Ornamental Plants. 5:1-5.
Tilkat, E., Onay, A., Yildirim, H. and Ayaz, E. (2009). Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L. Scientia Horticulturae. 121:361-365.
Tripathi, M. and Kumari, N. (2010). Micropropagation of a tropical fruit tree Spondias mangifera Willd. through direct organogenesis. Acta Physiol Plant. 32:1011-1015.
Wilhelm, E. (1999). Micropropagation of juvenile sycamore maple via adventitious shoot formation by use of Thidiazuron. Plant Cell, Tissue and Organ Culture. 57:57-60.
Wiriyaudom, S., Karuhapattana, B., Kittibanpacha, K., Sompoh, B., Bhodhipuks, J., Jitjak, J. and Mokkhunthod, S. (2012). The study of resin tapping improvement for Gluta usitata. Bangkok, Thailand.
Zipcodezoo. (2014). Melanorrhoea usitata. [Online] Retrieved from http://zipcodezoo.com/index.php/Melanorrhoea_usitata#cite_note-zipcodezoo.
