Streptomyces mediated stimulation of defense related enzymes to increase the biocontrol resistance in Capsicum annuum L. against Ralstonia solanacearum
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Abstract
The present study evaluated the selected rhizosphere soil Streptomyces cultures for the stimulation of defense enzymes in Capsicum annuum L. against Ralstonia solanacearum. Among the seven Streptomyces strains evaluated for the promotion of plant growth in pot experiments, strain UP1A-1 showed highest shoot length (16.8 cm), root length (12.1 cm), fresh weight (7.61 gm) and dry weight (0.81 gm). Similarly, the maximum amount of total chlorophyll was observed in the chili plant treated with the strain UP1A-1 (1.9 mg g-1) and UT2A-30 (1.59 mg g-1). The Streptomyces strains UP1A-1 (88.8%), UP2A-9 (87.0%) and UT6A-57 (83.2%) showed maximum biocontrol efficacy against R. solanacearum. The maximum phenolic content was observed in the chili pepper plants treated with the strain UP1A-1 at 9 days after pathogen inoculation (DPI). Also the plant defense related enzymes peroxides (POX) and polyphenol oxidase (PPO) significantly increased in UP1A-1 treated chili pepper at 3 DPI and 6 DPI. It is also noticed that initially at 1 DPI all treatments showed least amount of POX and PPO, later increased at 3 DPI and 6 DPI but suddenly started decreasing at 9 DPI in chili pepper. In conclusion, the current pot experiment indicated that the Streptomyces sp. UP1A-1 exhibited the maximum chili pepper growth, good stimulation of defense related enzymes and disease reduction against bacterial wilt and it could be a promising PGP and Biocontrol agent for chilli pepper plants against R. solanacearum.
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References
Abbasi, S., Safaie, N., Sadeghi, A. and Shamsbakhsh, M. (2019). Streptomyces Strains Induce Resistance to Fusarium oxysporum f. Sp. Lycopersici Race 3 in Tomato through Different Molecular Mechanisms. Frontiers in Microbiology, 10:1505.
Alizadeh, O., Azarpanah, A. and Ariana, L. (2013). Induction and modulation of resistance in crop (arbuscular mycorrhiza) and hormonal elicitors and plants against disease by bioagent fungi plant growth promoting bacteria. International Journal of Farming and Allied Sciences, 2:982-998.
Amaresan, N., Jayakumar, V., Kumar, K. and Thajuddin, N. (2011). Endophytic bacteria from tomato and chili, their diversity and antagonistic potential against Ralstonia solanacearum. Archives of Phytopathology and Plant Protection, 1-12.
Andersen, E. J., Ali, S., Byamukama, E., Yen, Y. and Nepal, M. P. (2018). Disease resistance mechanisms in plants. Genes, 9:339.
Arora, H., Sharma, A., Sharma, S., Haron, F. F., Gafur, A., Sayyed, R. Z. and Datta, R. (2021). Pythium damping-off and root rot of capsicum annuum l.: Impacts, diagnosis, and management. Microorganisms, 9:823.
Boukaew, S., Chuenchit, S. and Petcharat, V. (2011). Evaluation of Streptomyces spp. for biological control of Sclerotium root and stem rot and Ralstonia wilt of chili pepper. BioControl, 56:365-374. https://doi.org/10.1007/s10526-010-9336-4.
Chakrabarty, S., Islam, A. K. M. M. and Islam, A. K. M. A. (2017). Nutritional Benefits and Pharmaceutical Potentialities of Chili. Fundamental and Applied Agriculture, 2:272-232.
Enebe, M. C. and Babalola, O. O. (2019). The impact of microbes in the orchestration of plants’ resistance to biotic stress: a disease management approach. Applied Microbiology and Biotechnology, 103:9-25. https://doi.org/10.1007/s00253-018-9433-3
Geetha, R. and Selvarani, K. (2017). A Study of Chili Production and Export From India. International Journal of Advance Research and Innovative Ideas in Education, 3:2395-4396.
Guo, J. H, Qi, H. Y., Guo, Y. H., Ge, H. L., Gong, L. Y., Zhang, L. X. and Sun, P. H. (2004). Biocontrol of tomato wilt by plant growth-promoting rhizobacteria. Biological Control, 29:66-72.
Guo, Y., Fan, Z., Yi, X., Zhang, Y., Khan, R. A. A. and Zhou, Z. (2021). Sustainable management of soil-borne bacterium ralstonia solanacearum in vitro and in vivo through fungal metabolites of different trichoderma spp. Sustainability (Switzerland), 13:1491.
Harir, M., Bendif, H., Bellahcene, M., Fortas, Z. and Pogni, R. (2019). Streptomyces Secondary Metabolites. Retried from: http://dx.doi.org/10.5772/intechopen.79890
Islam, A. H. M. S., Schreinemachers, P. and Kumar, S. (2020). Farmers’ knowledge, perceptions and management of chili pepper anthracnose disease in Bangladesh. Crop Protection, 133:105139.
Jinwen, L., Jingping, Y., Pinpin, F., Junlan, S., Dongsheng, L., Changshui, G. and Wenyue, C. (2009). Responses of rice leaf thickness, SPAD readings and chlorophyll a/b ratios to different nitrogen supply in paddy field. Field Crops Res, 114:426-432.
Kumar, M., Srinivasa, V. and Kumari, M. (2018). Screening of Tomato Line / Varieties for Bacterial Wilt ( Ralstonia solanacearum ) Resistance in Hill Zone of Karnataka , India. International Journal of Current Microbiology and Applied Sciences, 7:1451-1455.
Lattanzio, V., Lattanzio, V. M. T. and Cardinali, A. (2006). Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: Imperato F (ed) Phytochemistry: advances in research. Research Signpost, Kerala, pp.26-67.
Li, L. and Steffens, J. C. (2002). Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta, 215:239-247.
Lichtenthaler, H. K. and Babani, F. (2004) Light adaptation and senescence of the photosynthetic apparatus: Changes in pigment composition, chlorophyll fluorescence parameters and photosynthetic activity. In Chlorophyll a Fluorescence: A Signature of Photosynthesis ed. Papageorgiu, E. and Govindjee, G.C. pp.713-736. Dordrecht, The Netherlands: Springer.
Ling, L., Han, X., Li, X., Zhang, X., Wang, H., Zhang, L., Cao, P., Wu, Y., Wang, X., Zhao, J. and Xiang, W. (2020). A streptomyces Sp. NEAU-HV9: Isolation, identification, and potential as a biocontrol agent against ralstonia solanacearum of tomato plants. Microorganisms, 8:351.
Madala, N. and Nutakki, M. K. (2020). Hot Pepper-History-Health and Dietary Benefits & Production. International Journal of Current Microbiology and Applied Sciences 9:2532-2538.
Małolepsza, U. (2006). Induction of disease resistance by acibenzolar- S-methyl and o-hydroxyethylorutin against Botrytis cinerea in tomato plants. Crop Protection 25:956-962.
Manda, R. R., Addanki, V. A. and Srivastava, S. (2020). Bacterial wilt of solanaceous crops. International Journal of Chemical Studies, 8:1048-1057.
Mandal, S. and Mitra, A. (2007). Reinforcement of cell wall in roots of Lycopersicon esculentum through induction of phenolic compounds and lignin by elicitors. Physiol Mol Plant Pathol, 71:201-209.
Manickam, R., Chen, J. R., Sotelo-Cardona, P., Kenyon, L. and Srinivasan, R. (2021). Evaluation of different bacterialwilt resistant eggplant rootstocks for grafting tomato. Plants, 10:75.
Ni, Z., Kim, E. D. and Chen, Z. J. (2009). Chlorophyll and starch assays. Protocol Exchange, 1-5. https://doi.org/10.1038/nprot.2009.12.
Olanrewaju, O. S. and Babalola, O. O. (2019). Streptomyces: implications and interactions in plant growth promotion. Applied Microbiology and Biotechnology, 103:1179-1188.
Pavlovic, D., Nikolic, B., Durovic, S., Waisi, H., Andelkovic, A. and Marisavljevic, D. (2014) Chlorophyll as a measure of plant health: Agroecological aspects. Pestic Phytomed (Belgrade), 29:21-34.
Priya, B. D. (2020). An investigation on production and productivity export performance of significant spices in the Country India. Indian Journal of Science and Technology, 13:4699-4707.
Ramamoorthy, V., Viswanathan, R., Raguchander, T., Prakasam, V. and Samiyappan, R. (2001). Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protection, 20:1-11.
Sakthivel, K., Gautam, R. K., Kumar, K., Dam Roy, S., Kumar, A., Devendrakumar, C., Vibhuti, M., Neelam, S. and Vinatzer, B. A. (2016). Diversity of ralstonia solanacearum strains on the Andaman Islands in India. Plant Disease, 100:732-738.
Salla, T. D., Astarita, L. V. and Santarém, E. R. (2016). Defense responses in plants of Eucalyptus elicited by Streptomyces and challenged with Botrytis cinerea. Planta, 243:1055-1070.
Sharma, V., Kaur, R. and Salwan, R. (2021). Streptomyces: host for refactoring of diverse bioactive secondary metabolites. 3 Biotech, 11:340.
Senthilraja, G., Anand, T., Kennedy, J. S., Raguchander, T. and Samiyappan, R. (2013). Plant growth promoting rhizobacteria (PGPR) and entomopathogenic fungus bioformulation enhance the expression of defense enzymes and pathogenesis-related proteins in groundnut plants against leafminer insect and collar rot pathogen. Physiological and Molecular Plant Pathology, 82:10-19.
Singh, S. and Balodi, R. (2021). Bio-management of soil borne pathogens infesting cucumber (Cucumis sativus L.) under protected cultivation system. Biological Control, 157:104569.
Srinivas, V., Gopalakrishnan, S., Kamidi, J. P. and Chander, G. (2020). Effect of Plant Growth-Promoting Streptomyces Sp . on Plant Growth and Yield of Tomato and Chili. Andhra Pradesh The Journal of Agricultural Science, 6:65-70.
Thilagam, R. and Hemalatha, N. (2019). Plant growth promotion and chili anthracnose disease suppression ability of rhizosphere soil actinobacteria. Journal of Applied Microbiology, 126:1835-1849.
Tsopoe, J. and Murry, N. (2020). Economics of Chili Cultivation in Wokha District of Nagaland, India. Current Agriculture Research Journal, 8:46-51.
Vanitha, S. C. and Umesha, S. (2008). Variations in defense related enzyme activities in tomato during the infection with bacterial wilt pathogen. Journal of plant interactions, 3:245-253.
Wuryandari, Y., Wiyatiningsih, S. and Sulistyono, A. (2016). The effect of fluorescent pseudomonads application on the resistance of chili plants against the attack of Ralstonia solanacearum and Fusarium oxysporum in the field. MATEC Web of Conferences, 58:01025.
Yadav, M., Dubey, M. K. and Upadhyay, R. S. (2021). Systemic resistance in chili pepper against anthracnose (Caused by Colletotrichum truncatum) induced by Trichoderma harzianum, trichoderma asperellum and Paenibacillus dendritiformis. Journal of Fungi, 7:307.
Yuliar, Asi Nion, Y. and Toyota, K. (2015). Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes and Environments, 30:1-11.
Zamocky, M., Regelsberger, G., Jakopitsch, C. and Obinger, C. (2001). The molecular peculiarities of catalase-peroxidases. FEBS Letters, 492:177-182.
Zieslin, N. and Ben-Zaken, R. (1993). Peroxidase activity and presence of phenolic substances in peduncles of rose flower. Plant Physiology and Biochemistry, 31:333-339.
Zhao, J., Han, L., Yu, M., Cao, P., Li, D., Guo, X., Liu, Y., Wang, X. and Xiang, W. (2019). Characterization of Streptomyces sporangiiformans sp. Nov., a novel soil actinomycete with antibacterial activity against Ralstonia solanacearum. Microorganisms, 7:360.
Zhuang, X., Gao, C., Peng, C., Wang, Z., Zhao, J., Shen, Y. and Liu, C. (2020). Characterization of a novel endophytic actinomycete, Streptomyces physcomitrii sp. nov., and its biocontrol potential against Ralstonia solanacearum on tomato. Microorganisms, 8:2025.