Morphological identification of plant parasitic nematodes associated with Rambutan (Nephelium lappaceum Linn.)
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Abstract
Plant-parasitic nematodes are one of the significant crop problems affecting various crop productions worldwide. Rambutan (Nephelium lappaceum) is one of the most economically important crops in the Philippines which its association with plant parasites is not well known. This paper aims to identify the species of plant-parasitic nematodes associated with rambutan as well as the manifestation of these plant parasites related to this fruit tree. The nematodes from soil and roots samples were extracted using the Baermann tray method and were identified under the light microscope based on their morphological characters. Four species of plant-parasitic nematodes were identified, namely: Discocriconemella spp., Hemicycliophora spp., Helicotylenchus spp. and Heterodera/Globodera spp. Where in Discocriconemella spp. was the most frequently occurring species in the soil with a total of 40%. The prevalence of Discocriconemella spp. indicates that the soil environment in the study area is favorable for these organisms but also suggest that abiotic factors such as Physico-chemical properties do have a relationship with the frequency of these plant-parasitic nematodes. Further studies, including molecular analysis and a vast number of samples, were recommended for accurate and specific identification of the species of the nematodes as well as utilizing multiple parameters to allow more statistical analysis about its morphometrics
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References
Barstow, M. (2017). Nephelium lappaceum. The IUCN Red List of Threatened Species 2017: e.T33266A67808476. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.20173.RLTS. T33266A67808476.en
Bert, W., Leliaert, F., Vierstraete, A. R., Vanfleteren, J. R. and Borgonie, G. (2008) Molecular phylogeny of the Tylenchina and evolution of the female gonoduct (Nema-toda: Rhabditida). Molecular Phylogenetics, 48:728-744.
Brooks, F. E. (2004). Plant-parasitic nematodes of banana in American Samoa. Nematropica, 34: 65-72.
Cantalapiedra-Navarrete, C., Navas-Cortés, J. A., Liébanas, G., Vovlas, N., Subbotin, S. A., Palomares-Rius, J. E. and Castillo, P. (2013). Comparative molecular and morphological characterisations in the nematode genus Rotylenchus: Rotylenchus paravitis n. sp., an example of cryptic speciation. Zoologischer Anzeiger, 252:246-268.
CIP (n.d.) “Nematodes”. Centro Internacional de la Papa. Retrieved from http://www.cipotato.org/potato/Pests_ Disease/IPM/ Pests/nema.html
Coyne D. L., Cortada, L., Dalzell, J. J., Claudius-Cole, S. G., Luambano, N. and Talwana, H. (2018). Plant-parasitic nematodes and food security in sub-saharan Africa. Annual Review of Phytopathology, 56:381-403.
Crow, W. T. (2014). Spiral Nematode Helicotylenchus spp. (Nematoda: Tylenchida: Hoplolaimidae). IFAS Extension, University of Florida.
Davide, R. G. (1988). Nematode Problems Affecting Agriculture in the Philippines. The Journal of Nematology, 20:214-218.
Davis, M. J., Gillaspie, A. G. Jr., Vidaver, A. K. and Harris, R. W. (1984). Clavibacter: A new genus containing some phytopathogenic coryneform bacteria including Clavibacter xyli subsp. xyli sp. nov.; subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov.; pathogens that cause ratoon stunting disease of sugarcane and Bermudagrass stunting disease. International Journal of Systematic Bacteriology, 34:107-117.
Dasgupta, D. R., Raski, D. J. and Van Gundy, S. D. (1969). Revision of the genus Hemicriconemoides Chitwood and Birchfield, 1957. (Nematoda: Criconematidae). Journal of Nematology, 1:126-145.
De Grisse, A. and Loof, P. A. A. (1965). Revision of the genus Criconemoides (Nematoda: Tylenchida). Mededelingen van de Landbouwhogeschool en de Opzoekingsstations van de Staat te Gent, 38:1303-1328.
Eisenback, J. D. (2002). Identification guides for the most common genera of plant-parasitic nematodes. Blacksburg, Virginia: Mactode Publications.
Gazaway, W., Hagan, A. K., Mullen, J. M. and Sikora, A. J. (1995). Collecting Soil and Root Samples for Nematode Analysis. The Alabama Cooperative Extension System (Alabama A&M University and Auburn University).
Geraert, E. (2010). The Criconematidae of the World. Identification of the Family Criconematidae (Nematoda). Ghent, Academia Press. pp.615.
Gressel, J., Hanafi, A., Head, G., Marasas, W., Obilana, A. B, Ochanda, J., Souissi, T. and Tzotzos, G. (2004). Major heretofore intractable biotic constraints to African food security that may be amenable to novel biotechnological solutions. Crop Protection, 23:661-689.
Gutiérrez-Gutiérrez, C., Palomares-Rius, J. E., Cantalapiedra-Navarrete, C., Landa, B. B., Esmenjaud, D. and Castillo, P. (2010). Molecular analysis and comparative morphology to resolve a complex of cryptic Xiphinema species. Zoologica Scripta, 39:483-498.
Inserra, R. N., Stanley, J. D., Ochoa, A., Schubert, T. S., Subbotin, S. A., Crow, W. T. and McSorley, R. (2014). Hemicriconemoides Species as Crop Damaging Parasitic Nematodes in Florida. Nematology Circular No. 223.
Kashi, L. and Karegar, A. (2014): Description of Helicotylenchus persiaensis sp. n. (Nematoda: Hoplolaimidae) from Iran. Zootaxa, 3785:575-588.
Kibet, T. K., Kinyua, J., Kariuki, D., Mamati, E. G., Onguso, J. and Wafula, E. (2014). Occurrence of Fungivorous Nematodes in Ngere Tea Catchment Area, Murang’a County, Kenya. International Journal of Life Sciences Research, 2:89-96.
Loos, C. A. (1949). Notes on free-living and plant-parasitic nematodes of Ceylon – No.4. Journal of the Zoological Society of India, 1:17-22.
Mackesy, D., Molet, T. and Sullivan, M. (2014). CPHST Pest Datasheet for Globodera rostochiensis, USDA-APHIS-PPQ-CPHST
Mekete, T., Dababat, A., Sekora, N., Akyazi, F. and Abebe, E. (comps). (2012). Identification key for agriculturally important plant-parasitic nematodes Prepared for the International Nematode Diagnosis and Identification Course 2012 - A manual for nematology. Mexico, D.F.: CIMMYT.
McCauley, A., Jones, C. and Jacobsen, J. (2011). Nutrient Management Module. Plant Nutrient Functions and Deficiency and Toxicity Symptoms, 9.
McSorley, R., Campbell, C. W. and Goldweber, S. (1980). Observations on a mango decline in south Florida. Proceedings of Florida State Horticultural Society, 93:132-133.
Morton, J. (1987). In: Fruits of warm climates. Julia F. Morton, Miami, FL. Rambutan. pp.262-265.
Nicol, J. M., Turner, S. J., Coyne, D. L., den Nijs, L., Hockland, S. and Tahna Maafi, Z. (2011) Current nema-tode threats to world agriculture. In: Jones, J., Gheysen, G. and Fenoll, C. (eds) Genomics and Molecular Genetics of Plant-Nematode Interactions. Springer, Dordrecht, the Netherlands, pp.21-43.
Riffle, J. W. (1962). Nematodes associated with maple dieback and maple blight. Phytopathology, 52:749.
Sabaratnam, S. (2012). Potato Cyst Nematodes. British Columbia Ministry of Agriculture. Accessed from: http://www.agf.gov.bc.ca/cropprot/goldennema.html
Siddiqi, M. R. (2000). Tylenchida parasites of plants and insects, 2nd edn. Wallingford, UK: CABI Publishing.
Spaull, A. M. and Newton, P. G. (1982). Numbers of root-ectoparasitic nematodes under some forage crops. Nematologica, 28:450-452.
Subbotin, S. A., Mundo-Ocampo, M. and Baldwin, J. G. (2010). Systematics of cyst nematodes. (Nematoda: Heteroderinae). Hunt, D. J. and Perry, R. N. (Eds.), Nematology Monographs and Perspectives 8B, the Netherlands: Leiden pp. 512.
Subbotin, S. A., Chitambar, J. J., Chizhov, V. N., Stanley, J. D., Inserra, R. N., Doucet, M. E., McClure, M., Ye, W., Yeates, G. W., Mollov, D. S., Cantalapiedra-Navarette, C., Volvas, N., Van Den Berg, E. and Castillo, P. (2014). Zoological Journal of the Linean Society. Molecular Phylogeny, Diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
Tarjan, A. C. (1952). The nematode genus Hemicycliophora de Man, 1921 (Criconematidae) with a description of a new plantparasitic species. Proceedings of the Helminthological Society of Washington, 19:65-77.
Tarjan, A. C. (1977). Application of municipal solid waste compost to nematode-infected citrus was an attempt to put to good use solid waste compost, otherwise known as "garbage". The material applied was "supersoil", a product made of thoroughly digested and pulverized municipal waste. Results indicated a slight beneficial effect as measured by growth response, plsnt appearance and fruit yield. Nematropica, 7:53-56.
Thorne, G. (1961). Principles of nematology. New York: McGraw-Hill Book Company.
Van Gundy, S. D. and Rackham, R. L. (1961). Studies on the biology and pathogenicity of Hemicycliophora arenaria. Phytopathology, 51:393-397.
Vovlas, N. (1992). Taxonomy of Discocriconemella (Nematoda: Criconematoidea) with a Redescription of D. mauritiensis. J. Nematol, 24:391-398.
Whitlock, L. S. and Steele, A. E. (1960). Notes on Hemicriconemoides gaddi from camellias in Louisiana and Georgia. Plant Disease Reporter, 44:446-447.
World Bank (2008). World Development Report 2008: Agriculture for Development. The World Bank, Washington.