Analysis of rhizobacterial community in field grown GM and non-GM maize soil samples using PCR-DGGE
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Abstract
This study assessed the impact of Bacillus thuriengensis genetically modified maize (GM Bt maize) on functional community of rhizobacteria. Denaturing gradient gel electrophoresis technique (DGGE) was used. Results indicated that band patterns of GM and non-GM samples were similar to each other at 30 days after sowing (DAS) and 1 day after harvest (DAH). Sequence analysis showed that uncultured bacteria, Actinobacterium, Bradyrhizobium spp., and Sphingomonas spp., were common in the soil samples indicating that these were a stable community and species. These findings showed that the GM maize did not alter the soil microbial community which is significant in the assessment of the impact of GM maize on rhizobacteria.
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