Micropropagation of Lilium formolongo via leaf explants
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Abstract
The effect of cultured media and plant growth regulator on micropropagation from the leaf of Lilium formolongo was cultured on three basic media; Murashige and Skoog (1962) (MS), 1/2MS and Nitsch and Nitsch (1969) (NN) media in the presence and absence of plant growth regulator 1.0 mg/l a-naphthaleneacetic acid (NAA) and 0.5mg/L Thiadiazuron (TDZ). The leaf explants that were cultured on the medium without plant growth regulator died within four weeks. Callus formation was obtained from the leaf explants cultured on the medium supplemented with plant growth regulator after culturing under dark condition for four weeks. Callus cultured on the MS medium with 1.0 mg/L NAA and 0.5 mg/L TDZ gives the best average size of 2.93 centimeters. The callus was then continuously cultured on the same medium but under light condition for 16 hours/day leading to shoot development. After 14 weeks incubation, 5.05 shoots and 36.5 leaves were formed.
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