Isolation and screening for inhibitory activity on Alternaria brassicicola of endophytic actinomycetes from Centella asiatica (L.) Urban

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Published: May 15, 2015
Keywords:
Centella asiatica (L.) Urban Endophytic actinomycetes Alternaria brassicicola

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Phuakjaiphaeo, C.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Kunasakdakul, K.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand

Abstract

A total of 36 actinomycete strains were isolated from Centella asiatica (L.) Urban on IMA-2 medium. There were 19 strains from stolons, nine strains from nodes with roots, four strains from leaves and four strains from fruits. The cultural characteristics on the isolation medium were used to divide all 36 strains into eight groups based on the reverse color of the streaked-substrate, color and growth rate of their aerial mycelia. Spore chain types were also determined, and the results showed that all groups generally produced rectus-flexibilis, retinaculum-apertum and spiral types and did not produce any diffusible pigment in the medium. Their antifungal activity against Alternaria brassicicola was screened using the dual culture method. Nine strains (25%) from various plant parts exhibited high inhibitory activity (73.40-80.0%) against the pathogen.  The rest of the strains showed no activity or weak to moderate inhibitory activity against A. brassicicola. Results in inhibition of the pathogen radial growth indicated that 36.11% of the strains showed moderate inhibitory activity (66.79% − 73.39%). In addition, strain CEN26 isolated from a node with roots was selected to determine its antifungal activity by microscopic observation. Morphological disorders of the pathogen, swollen hyphae and frequent septa of the treated pathogen mycelia were found after dual culture testing for 6 days compared to the normal growth in control treatment.

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Phuakjaiphaeo, C., & Kunasakdakul, K. (2015). Isolation and screening for inhibitory activity on Alternaria brassicicola of endophytic actinomycetes from Centella asiatica (L.) Urban. International Journal of Agricultural Technology, 11(4), 903–912. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6387
Issue
Vol. 11 No. 4 (2015): May
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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