Testing of the efficacy of dietary natural and synthetic antioxidants on broiler performance: A comparative study
Main Article Content
Abstract
A comparative study was conducted to test of the efficacy of dietary natural and synthetic antioxidants on productive performance, blood biochemical composition and liver oxidation status of broiler chickens. One-day old Ross broiler chickens(n=210)were fed together on basal diet during the first week of age, then divided randomly into seven treated groups:T0(control group) without any addition,T1=0.75g Withania somnifera leaves(WSL)/kg diet,T2=1.5gWSL/kg diet,T3=0.75g rosemary leaves(RL)/kg diet,T4=1.5gRL/kg diet,T5=0.75g vitamin E/kg diet and T6=1.5g vitamin E/kg diet. Results showed that supplementing broilers with 1.5g WSL significantly increased final live body weight, body weight gain, dressing percentage and improved feed conversion ratio. Results of serum biochemical tests indicated that dietary supplementation with natural and synthetic antioxidants significantly (p≤0.05) reduced serum cholesterol as compared to control group. High levels of RL and vitamin E significantly decreased LDL as compared to control. Dietary supplementation with 1.5g RL significantly decreased serum albumin and glucose and increased globulin as compared to vitamin E supplemented groups. No significant differences were found between all experimental groups in serum HDL, VLDL, triglycerides, total protein, AST and ALT. In liver,1.5g WSL significantly reduced peroxide value and malondialdehyde levels as compared to RL. It can be concluded from this study that adding herbs like Withania somnifera as natural antioxidant to broiler diet had positive effect in reducing lipid oxidation and minimize harmful effect of oxidation damage.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Anabalagan, K. and Sadique, J. (1981). Influence of an Indian medicin (Ashwagandha) on acute phase reactions in inflammation. Indian Journal of Experimental Biology 19:245.
Anderson, J. W. and Tietyen-Clark, J. (1986). Dietary fiber: hyperlipidemia, Hypertension and coronary heart disease. The American Journal of Gastroenterology 81:907-919.
Arsalan, M., Ozcan, M., Matur, E., Cotelioglu, U. and Ergul, E. (2001). The effect of vitamin E on some blood parameters in broilers. Turkish Journal of Veterinary and Animal Sciences 25:711-716.
Bhattacharya, S. K., Satyam, S. K. and Chakrabarti, A. (1997). Effect of Trasina and Ayurvedic herbal formulation on pancreatic islet superoxide dismutase activity in hyperglycaemic rats. Indian Journal of Experimental Biology 35:297-299.
Cadens, E. and Davies, K. J. A. (2000). Mitochondrial free radical generation, oxidative stress and aging. Free Radic. Biology and Medicine 29:222-230.
Devipriya, S. and Shyamaladevi, C. S. (1999). Protective effect of quercetin in cisplatin induced cell injury in the rat kidney. Indian Journal of Pharmacology 31:422-426.
Dhuley, J. N. (2000). Adaptogenic and cardioprotective action of Ashwagandha in rats and frogs. Journal of Ethnopharmacology 70:57-63.
Duncan, D. B. (1955). Multiple ranges and multiple F test. Biometrics 11:1-42.
Ebihara, K. and Schneeman, B. O. (1989). Interaction of bile acids, phospholipids, cholesterol and triglyceride with dietary fibers in the small intestine of rats. The Journal of Nutrition 119:1100-1106.
Egan, H., Kirk, R. S. and Sawyer, R. (1981). Pearson's chemical analysis of foods. Edinburgh, UK: Churchill Livingstone.
Faixova, Z. and Faix, S. (2008). Biological effect of rosemary essential oil. Review. Folia Veterin 52:135-139.
Fellenberg, M. A. and Speisky, H. (2006). Antioxidants: their effect on broiler oxidative stress and its meat oxidative stability. World's Poultry Science 62:1-13.
Ghazalah, A. A. and Ali, A. M. (2008). Rosemary leaves as a dietary supplement for growth in broiler chickens. Inter. The Journal of Poultry Science 7:234-239.
Halliwell, B., Aeoschbach, R., Loliger, J. and Aruoma, O. I. (1995). The characterization of antioxidants. Food and Chemical Toxicology 33:601-617.
Halliwell, B. and. Gutteridge, J. M. C. (1999). Free radicals in biology and medicine, 3rd ed. Oxford: Clarenson Press. pp. 416-494.
Ho, C. T. M., Wang, F., Wei, G. J., Huang T. C. and Huang, M. T. (2000). Chemistry and antioxidative factors in rosemary and sage. Proceedings of the 2nd International Conference on Food Factors (Ico FF, 99). 12-17 December, 1999, Kyoto, Japan. Bio-Factors 13:161-166.
Huang, J., Nasr, M., Kim, Y. and Matthews, H. R. (1992). Genistein inhibit protein histidine kinase. The Journal of Biological Chemistry 267:15511-15515.
Houghton, J., Zarka, R., Heras, B. I. and Houtt, J. R. S. (1995) S. sa Fixed oil of nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Plant Medica 61:33-36.
Hutadilok-Towatana, N., Chaiyamutti, P., Panthong, K., Mahabusarakam, W. and Rukachaisrikul, V. (2006). Antioxidative and free radical scavenging activities of some plants used in Thai Folk Medicin. Pharmaceutical Biology 44:221-228.
Lansky, P. S., Schilcher, H., Phillipson, J. D. and Loew, D. (1993). Plant that lower cholesterol. First Worid Congress on Medicinal and Aromatic Plants (WOCMAP) for human welfare, Maastricht, Netherlands, Acta-Horticulturae 332:131-136.
Khare, C. P. (2007). Indian Medicinal Plants-An Illustrated Dictionary. First Indian Reprint, Springer (India) Pvt. Ltd., New Delhi. pp. 717-718.
Meyorrissey, P. A., Sheehy. P. J. E., Galven, K., Kerry, J. P. and Buckley, D. J. (1998). Lipid stability in meat and meat products. Meat Science 49:S73-S86.
McCarthy, T. L. Kerry, J. P., Kerry, J. F., Lynch P. B. and Bucckley, D. J. (2001). Evaluation of the antioxidant potential of natural food/plant extracts as compared with synthetic antioxidants and vitamin E in raw and cooked pork patties. Meat Science 57:45-52.
McDowel, L. R. (1989). Vitamins in animal nutrition: Comparative aspects to human nutrition. Academic Press Limited.
Mohammed, T. T. (2013). The effect of adding different levels of artificial antioxidants and natural antioxidants in the diet on production and physiological performance in laying hens. (Doctor of Philosophy’s Thesis). College of Agriculture, University of Al-Anbar.
More, T., Rai, A. K. and Singh, M. (1980). Note on the effect of thermal exposure on body fluid composition of different breeds and crosses of sheep. Indian Journal of Animal Sciences 50:207-209.
National Research Council (NRC) (1994). Nutrient requirement of poultry. 9th revisited National academy press, Washington D. C., USA.
Nielsen, S. E., Young, J. F., Daneshvar, B., Lauridsen, S. T., Knuthsen, P., Sandstorm B. and Dragsted, L. O. (1999). Effect of parsley (Petraselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects. British Journal of Nutrition 81:447-455.
Parker, L. and Landvik, S. (1990). Vitamin E in biological systems. In: Emerit I., Parker L., Auclair (eds.): Antioxidants in Therapy and Preventive Medicine. Plenum, New York: pp. 93-103.
Polat, U., Yesilbag, D. and Eren, M. (2011). Serum biochemical profile of broiler chickens fed diets containing rosemary and rosemary volatile oil. Journal of Biodiversity and Environmental Sciences 5:23-30.
Radwan, N. L., Hassan, R. A., Qota. E. M. and Fayek, H. M. (2008). Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. International Journal of Poultry Science 7:134-150.
SAS(Statistical Analysis System). (2010). Users Guide. Statistical Version 6th ed, SAS. Inst. Inc. Cary. NC, USA.
Shahin, K., Shahin, N., Onderci, M., Yaralioglu S. and Kucuk, O. (2001). Protective role of supplemental vitamin E on lipid peroxidation , vitamins E, A and some mineral concentrations of broilers reared under heat stress. Veterinary Medicine, Czech 46:140-144.
Shehata, A. M. and Yousef, O.M. (2010). Physiological studies on the risk factors responsible for atherosclerosis in rats. Journal of Nature and Science 8:144-151.
Sudheesh, S., Sandhyna, C., Saran, K. A. and Vijiayalakshmi, N. R. (1999). Antioxidant activity of flavonoids from Solanum melongena. Phytotherapy Research 13:393-396.
Sundaram, S., Dwivedi, P. and Purwar, S. (2011). In vitro evaluation of antibacterial activities of crude extracts of Withania somnifera (Ashwagandha) to bacterial pathogens. Asian Journal of Biotechnology 3:194-199.
Tekeli, A., Celik, L., Kultu, H. R. and Gorgulu, M. (2006). Effect of dietary supplemental plant extracts on performance, carcass characteristics, digestive system development, intestinal microflora and some blood parameters of broiler chicks. Proceedings of XII European Poultry Conference, Verona, Italy.
Uddin, Q., Samiulla, L., Singh, V. K. and Jamil, S. S. (2012). Phytochemical and pharmacological profile of Withania somnifera Dunal: A Review. Journal of Applied Pharmaceutical Science 2:170-175.
Witte, V. C., Krause, G. F. and. Bailey, M. E. (1970). A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. Journal of Food Science 35:582-585.
Zantop, D. W. (1997). Avian medicine: Principle and Applications. Wingers Publ. Inc., Lake Worth, FL. pp. 115-129.