In vitro screening of antagonistic activity of soil Streptomyces against plant pathogenic fungi and assessment of its characters
Article Sidebar
Main Article Content
Abstract
Actinobacteria from the genus Streptomyces have been studied as potential biocontrol agents worldwide. In this study, a total of 35 isolates of Actinomycete like colonies were isolated from the rhizospheres of various plant species. These isolates were primary screened for antagonistic activity to inhibit mycelial growth of Colletotrichum gloeosporioides. The screen revealed that seven isolates of Actinomycete like colonies were good inhibitors of the fungal mycelial growth and were chosen for their antagonistic effects against C. gloeosporioides and Biporalis maydis by the dual culture method. The isolate that had the best inhibitory effect on both fungi was further evaluated for antagonistic activity against C. capsici, Pyricularia sp., Fusarium sp., Curvularia sp. and Sclerotium roftsii. The results showed that the selected isolate inhibited the mycelial growth of C. capsici, Pyricularia sp., Fusarium sp. and Curvularia sp. within the range of 65.5-91.6%, while it did not affect the mycelial growth of S. roftsii. Moreover, the culture filtrate of the selected isolate inhibited the mycelial growth of C. gloeosporioides and B. maydis under in vitrocondition. Based on the 16S rDNA gene sequence, colony morphology, spore chain rearrangement and physiology, the best isolate belonged to the genus Streptomyces and assigned as Streptomyces sp. isolate SRF1. These findings suggest that the Streptomyces sp. isolate SRF1 is a potential candidate, with a broad antagonistic activity, for use as a biocontrol against plant pathogenic fungi.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Alam M., Dharni S., Khaliq A., Srivastava S. K., Samad A. and Gupta M. K. (2012). A promising strain of Streptomyces sp. with agricultural traits for growth promotion and disease management. Indian Journal of Experimental Biology 50:559-568.
Alvindia, D. G. and Natsuaki, K. T. (2008). Evaluation of fungal epiphytes isolated from banana fruit surfaces for biocontrol of banana crown rot disease. Crop Protection 27:1200-1207.
Boukaew, S., Chuenchit, S. and Petcharat, V. (2011). Evaluation of Streptomyces spp. for biological control of Sclerotium root and stem rot and Ralstonia wilt of chili pepper. BioControl 56:365-374.
Choi, S. H., Son, M. J., Kim, S. H., Choi, S. Y. and Lee, Y. H. (2009). Isolation and medium development of the actinomycetes, Streptomyces griseofucus CNU-A91231, inhibiting phytopathogenic fungi. Korean Journal of Microbiology and Biotechnology 37:322-332.
Cook, R. J. (1993). Making greater use of introduced microorganisms for biological control of plant pathogens. Annual Review of Phytopathology 31:53-80.
Cross, T (1989). Growth and examination of Actinomycetes some guidelines. In Bergey's Manual of Systematic Bacteriology. Williams and Wilkins Company, Baltimore 4, pp. 2340-2343.
Evangelista, M. (2014). Isolation and characterization of soil Streptomyces species as potential biological control agents against fungal plant pathogens. World Journal of Microbiology and Biotechnology 30:1639-1647.
Gohel, V., Singh, A., Vimal, M., Ashwini, P. and Chhatpar, H. S. (2006). Bioprospecting and antifungal potential of chitinolytic microorganisms. African Journal of Biotechnology 5:54-72.
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. and Williams, S. T. (2000). Bergey’s manual of determinative bacteriology, 9th edn. Lippincott Williams & Wilkins, Philadelphia.
Ilic, S. B., Konstantinovic, S. S., Todorovic, Z. B., Lazic, M. L., Veljkovic, V. B., Jokovic, N. and Radovanovic, B. C. (2007). Characterization and antimicrobial activity of the bioactive metabolites in Streptomyces isolates. Microbiology 76:421-428.
Janda, J. M. and Abbott, S. L. (2007). 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. Journal of Clinical Microbiology 45:2761-2764.
Joo, G. J. (2005). Production of an anti-fungal substance for biological control of Phyophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnology Letters 27:201-205.
Kaewkla, O. and Franco, C. M. M. (2013). Rational approaches to improving the isolation of endophytic actinobacteria from Australian native trees. Microbial Ecology 65:384-393.
Kim, H. J., Lee, S. C. and Hwang, B. K. (2006). Streptomyces cheonanensis sp. nov., a novel streptomycete with antifungal activity. International Journal of Systematic and Evolutionary 56:471-475.
Lee, E. J., Ahn, Y. J., Lee, H. S. and Chung, N. (2012). Biocontrol of pepper anthracnose by a new Streptomyces sp. A1022 under greenhouse condition. Journal of the Korean Society for Applied Biological Chemistry 55:447-449.
Moënne-Loccoz, Y., Tichy, H. V., O'Donnell, A., Simon, R. and O'Gara, F. (2001). Impact of 2,4-diacetylphloroglucinol-producing biocontrol strain Pseudomonas fluorescens F113 on intraspecific diversity of resident culturable fluorescent pseudomonads associated with the roots of field-grown sugar beet seedlings. Applied and Environmental Microbiology 67:3418-3425.
Ouhdouch, Y., Barakate, M. and Finance, C. (2001). Actinomycetes of Moroccan habitats: isolation and screening for antifungal activities. European Journal of Soil Biology 37:69-74.
Prapagdee, B., Akrapikulchart, U. and Mongkolsuk, S. (2008). Potential of a soil-borne Streptomyces hygroscopicus for biocontrol of anthracnose disease caused by Colletotrichum gloeosporioides in orchid. Journal of Biological Sciences 8:1187-1192.
Soares, A. C. F., da Silva Sousa, C., da Silva Garrido, M., Perez, J. O. and de Almeida, N. S. (2006). Soil Streptomycetes with in vitro activity against the yam pathogens Curvulara eragrostides and Colletotrichum gloeosporioides. Brazilian Journal of Microbiology 37:456-461.
Taechowisan, T., Lu, C., Shen, Y. and Lumyong, S. (2005) Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity. Microbiology 151:1691-1695.
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599.
Trejo-Estrada, S. R., Paszczynski, A. and Crawford, D. L. (1998). Antibiotics and enzymes produced by the biocontrol agent Streptomyces violaceusniger YCED9. Journal of Industrial Microbiology and Biotechnology 21:81-90.
Weisburg, S. T., Goodfellow, M., Wellington, E. M. H., Vickers, J. C., Alderson, G., Sneath, P. H. A., Sackin, M. J. and Mortimer, A. M. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173:697-703.
Woo, P. C. Y., Lau, S. K. P., Teng, J. L. L., Tse, H. and Yuen, K. Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical Microbiology and Infection 14:908-934.
ŽivkoviĆ, S., StojanoviĆ, S., IvanoviĆ, Ž., GavriloviĆ, V., PopoviĆ, T. and BalaŽ, J. (2010). Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides. Archives of Biological Science Belgrade 62:611-623.