Characteristics of epididymal sperm recovered from slaughterhouse derived testes of nondescript/native goats in the Philippines

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Published: Mar 15, 2016
Keywords:
Goat epididymal sperm viability and motility characteristics Post mortem testes

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Joram, J. G.
Central Luzon State University, College of Veterinary Science and Medicine, Science City of Munoz, Nueva Ecija Philippines 3120
Balagan, E. J. Y.
Central Luzon State University, College of Veterinary Science and Medicine, Science City of Munoz, Nueva Ecija Philippines 3120
Fely, V. M.
Philippine Carabao Center-Reproductive Biotechnology Unit, Science City of Munoz, Nueva Ecija Philippines 3120
Anaois, I. I.
Central Luzon State University, College of Veterinary Science and Medicine, Science City of Munoz, Nueva Ecija Philippines 3120
Ocampo, M. B.
Philippine Carabao Center-Reproductive Biotechnology Unit, Science City of Munoz, Nueva Ecija Philippines 3120, Affilitate Faculty IGS, Central Luzon State University, Science City of Munoz, Nueva Ecija, Philippines
Ocampo, L. C.
Philippine Carabao Center-Reproductive Biotechnology Unit, Science City of Munoz, Nueva Ecija Philippines 3120, Central Luzon State University, College of Veterinary Science and Medicine, Science City of Munoz, Nueva Ecija Philippines 3120

Abstract

 In this study, scrotal intact testes (n=6) were collected within 1-2 hour after slaughter from matured native bucks.  The objective of this study is to optimize a method of sperm collection from post mortem testes and determine the sperm characteristics using basic parameters such as motility, viability, sperm concentration, and sperm volume.   The slice + swim-up (Method 1) or Mince +flushing (Method 2) were performed to isolate epididymal sperm. In both methods, the obtained percentage sperm motility ranged between 60-75% by conventional method of assessment.   The average sperm concentration per mL was higher in Method 2 with 1.89 ± 0.36 x109sperm compared to  Method 1 with 1.23 ± 0.29 x109 but did not differ significantly  by Student’s T- test (p>0.05).  In terms of sperm volume,  an average  of 770±200 µl was recovered in  Method 2 compared with 500±110 µl in  Method 1 and are not  significantly different (p>0.05).   In terms of percentage live sperm at the time of collection,  Method 1 registered a higher mean percentage of 88.9±2.65 as compared with Method 2 with a mean percentage of  81.67±2.81 (Student’s T-test p>0.05). The proportion of normal sperm was 76.9±8.48 and 69.4±5.34 in Methods 1 and 2, respectively.  The presence of proximal (16-18%) and distal droplet (69-71%) was a common find  which is indicative of a sub population of maturing sperms in the epididymis. We therefore conclude that using the two recovery methods a considerable population of viable epididymal sperm can be isolated from post mortem gonads of matured nondescript bucks.

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Joram, J. G., Balagan, E. J. Y., Fely, V. M., Anaois, I. I., Ocampo, M. B., & Ocampo, L. C. (2016). Characteristics of epididymal sperm recovered from slaughterhouse derived testes of nondescript/native goats in the Philippines. International Journal of Agricultural Technology, 12(2), 215–228. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6587
Issue
Vol. 12 No. 2 (2016): March
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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