Phylogenetic diversity among Egyptian isolates of Fusarium species from sugar beet
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Abstract
Genetic variation was done to differentiate between Fusarium species endemic in sugar beet grown fields throughout different geographic locations of Egypt. TEF 1α, β- tubulin and histone 3 genes could separate obviously between F. oxysporum, F. solani, F. proliferatum, F. equiseti and F. veticillioides. TEF 1α was shown to be the best gene in this respect. As F. oxysporum and F. solani comprisethe most common pathogens of sugar beet in Egypt as well as other countries, the phylogenetic analyses were done by TEF 1α gene to correlate between the Egyptian isolates of these two species and the GenBank sequences representing species and clades. The phylogenetic TEF 1α tree separated the F. oxysporum into three clades and the Egyptian candidates placed in clade C.whereas, our isolates of F.solani aggregated into one group corresponding to clade 3.Phylogentic analysis could not reveal correlation among the geographic origin or the pathogenicity of Fusarium isolates on sugar beet.
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References
Aoki, T., O’Donnell, K. and Geiser, D. M. (2014). Systematics of key phytopathogenic Fusarium species: current status and future challenges. Journal of General Plant Pathology 80:189-201.
Aoki, T., O’Donnell, K. and Scandiani, M. M. (2005). Sudden death syndrome of soybean in South America is caused by four species of Fusarium: Fusarium brasiliense sp. nov., F. cuneirostrum sp. nov., F. tucumaniae, and F. virguliforme. Mycoscience 46:162-183.
Armstrong, G. M. and Armstrong, J. K. (1981). Formae speciales and races of Fusarium oxysporum causing wilt diseases. p. 391-399. In P.E. Nelson, T.A. Toussoun, and R.J. Cook (ed.) Fusarium:Diseases, Biology, and Taxonomy. The Pennsylvania State University Press, University Park.
Barik, B. P. and Tayung, K. (2012). Molecular differentiation of Fusarium spp. with varied lifestyles based on TEF 1 alpha gene sequence analysis. Interdisciplinary Sciences: Computational Life Sciences 4:201-208.
Bogale, M., Steenkamp, E. T., Wingfield, M. J. and Wingfield, B. D. (2009). Diverse Fusarium solani isolates colonise agricultural environments in Ethiopia. European Journal of Plant Pathology 124:369-378.
Burlakoti, P. (2007). Fusarium species associated with sugarbeet grown in the Red River Valley: Pathogenicity, cultivar response, and baseline sensitivity to fungicides. MS North Dakota State University.
Burlakoti, P., Rivera, V., Secor, G. A., Qi, A., Del Rio-Mendoza, L. E. and Khan, M. F. R. (2012). Comparative pathogenicity and virulence of Fusarium species on sugar beet. Plant Disease 96:1291-1296.
Campbell, L. G., Fugate, K. K. and Niehaus, W. S. (2011). Fusarium yellows affects postharvest respiration rate, sucrose concentration and invert sugar in sugar beet. The Journal of Sugar Beet Research 48:17-39.
Chehri, K., Salleh, B. and Zakaria, L. (2015). Morphological and Phylogenetic analysis of Fusarium solaniSpecies Complex in Malaysia. Microbial Ecology 69:457-471.
Covey, P. A., Kuwitzky, B., Hanson, M. and Webb, K. M. (2014). Multilocus analysis using putative fungal effectors to describe a population of Fusariumoxysporum from sugar beet. Phytopathology 104:886-896.
Cramer, R. A., Byrne, P. F., Brick, M. A. Panella, L., Wickliffe, E. and Schwartz, H. F. (2003). Characterization of Fusariumoxysporum isolates from common bean and sugar beet using pathogenicity assays and Random-amplified Polymorphic DNA markers. Journal of Phytopathol 151:352-360.
Desjardins, A. E., Munkvold, G. P., Plattner, R. D. and Proctor, R. H. (2002). FUM1 – a gene required for fumonisin biosynthesis but not for maize ear rot and ear infection by Gibberella moniliformis in field tests. Molecular Plant-Microbe Interactions Journal 15:1157-1164.
Fahmy, Z. M., Yasser M. M., Mousa, A. S. M., Taha, E. M. and Rabie, W. (2015). Fusarium species infecting sugar beet in Egypt. Journal of Applied Sciences 30:346-356.
Geiser, D. M., Jimenez-Gasco, M. M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T. J., Zhang, N., Kuldau, G. A. and O’Donnell, K. (2004). Fusarium-id v. 1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology 110:473-479.
Glass, N. L. and Donaldson, G. C. (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61:1323-1330.
Hanson, L. E. and Jacobsen, B. J. (2009). Fusarium yellows. Pages 28-29 in: Compendium of Beet Diseases and Pests. R. M. Harveson, L. E. Hanson, and G. L. Hein, eds. American Phytopathological Society Press, St. Paul, MN.
Hanson, L. E. and Hill, A. L. (2004). Fusarium species causing Fusarium yellows of sugarbeet. The Journal of Sugar Beet Research 41:163-178.
Harveson, R. M. and. Rush, C. M. (1997). Genetic variation among Fusariumoxysporum isolates from sugar beet as determined by vegetative compatibility. Plant Disease 81:85-88.
Hill, A. L., Reeves, P. A., Larson, R. L., Fenwick, A. L., Hanson, L. E. and Panella, L. (2011). Genetic variability among isolates of Fusarium oxysporum from sugar beet. Plant Pathology 60:496-505.
Khan, M., Bradley, C. A. and Windels, C. E. (2003). Fusarium yellows of sugar beet. Univ. Minn. Ext. Serv. and North Dakota State University. 1247 pp.
Kistler, H. C. (2001). Evolution of host specificity in Fusarium oxysporum, p. 70-96. In B. A. Summerell, J. F. Leslie, D. Backhouse, W. L. Bryden, and L. W. Burgess (eds.), Fusarium: Paul E. Nelson Memorial Symposium. APS Press, St. Paul, Minnesota.
Koenraadt, H., Somerville, S. C. and Jones, A. L. (1992). Characterization of mutations in the Beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Phytopathology 82:1348-1354.
Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. and Higgins, D. G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.
Lievens, B., Rep, M. and Thomma, B. P. (2008). Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest Management Science 64:781-788.
Nalim, F. A., Samuels, G. J., Wijesundera, R. L. and Geiser, D. M. (2011). New species from the Fusarium solani species complex derived from perithecia and soil in the Old World tropics. Mycologia 103:1302-1330.
Nitschke, E., Nihlgard, M. and Varrelmann, M. (2009). Differentiation of eleven Fusarium spp. isolated from sugar beet, using restriction fragment analysis of a polymerase chain reaction amplified translation elongation factor 1a gene fragment. Phytopathology 99:921-929.
O’Donnell, K. (2000). Molecular phylogeny of the Nectria haematococca-Fusariumsolani species complex. Mycologia 92: 919-938.
O’Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. (1998). Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences (USA) 95:2044-2049.
O’Donnell, K., Sutton, D. A., Fothergill, A., McCarthy, D., Rinaldi, M. G., Brandt, M. E., Zhang, N. and Geiser, D. M. (2008). Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusariumsolani species complex. Journal of Clinical Microbiology 46:2477-2490.
Saremi, H., Okhovvat, S. M. and Ashrafi, S. J., (2011). Fusarium diseases as the main soil borne fungal pathogen on plants and their control management with soil solarization in Iran. African Journal of Biotechnology 10:18391-18398.
Schmale, D. G. and Bergstrom, G. C. (2003). Fusarium head blight in wheat. The Plant Health Instructor. DOI:10.1094/PHI-I-2003-0612-01.
Smith, S. N. (2007). An Overview of Ecological and Habitat Aspects in the Genus Fusarium with Special Emphasis on the SoilBorne Pathogenic Forms. Journal of Plant Pathology 16: 97-120.
Stewart, D. (1931). Sugar-beet yellows caused by Fusarium conglutinans var. betae. Phytopathology 21:59-70.
Stojsin, V. B., Maric, A. A., Jasnic, S. M., Bagi, F. F. and Marinkovic, B. J. (2006). Root rot of sugar beet in the Vojvodina Province [Serbia (Serbia and Montenegro)]. Matica Srpska Proceedings for Natural Sciences 110:65-74.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30: 2725-2729.
Tomioka, K., Hirooka, Y., Takezaki, A., Aoki, T. and Sato, T. (2011) Fusarium root rot of prairie gentian caused by a species belonging to the Fusarium solani species complex. Journal of General Plant Pathology 77:132-135.
Webb, K. M., Case, A. J., Brick, M. A., Otto, K. and Schwartz, H. F. (2013). Cross pathogenicity and vegetative compatibility of Fusarium oxysporum isolated from sugar beet. Plant Disease 97:1200-1206.
Webb, K. M., Covey, P. A. and Hanson, L. E. (2012). Pathogenic and phylogenetic analysis of Fusarium oxysporum from sugar beet in Michigan and Minnesota. The Journal of Sugar Beet Research 49:38-56.
Windels, C. E., Brantner, J. R., Bradley, C. A. and Khan, M. F. R. (2005). First report of Fusarium oxysporum causing yellows on sugar beet in the Red River Valley of Minnesota and North Dakota. Plant Disease 89:341.