A molecular survey of Theileria spp. in Ruminants in the Thailand-Cambodia border region

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Published: Jan 15, 2022
Keywords:
Infection prevalence Ruminant Theileriosis Theileria parasites PCR technique

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Kaewhom, P.
Faculty of Agricultural Technology, Burapha University, Sa Kaeo Campus, Sa Kaeo, Thailand
Srikijkasemwat, K.
Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand

Abstract

Theileriosis is caused by Theileria spp. Parasites which transmitted by various species of ticks including Amblyomma, Haemaphysalis, Hyalomma and Rhipicephalus. This pathogen can be found in red blood cells and white blood cells of farmed animals, including cattle, buffaloes, goats and sheep. Theileria spp. leads to a high morbidity in small ruminants, economic loss in agricultural production and time spent on infection prevention. It is necessary to detect the infection in a farm, as there are generally no clearly recognisable clinical signs. Our results revealed that infected animals displayed 230 bp DNA fragments, which is the length of the V4 region of the 18S rRNA gene of Theileria spp.  The overall prevalence of Theileria spp. in ruminants farmed in Sa Kaeo Province, the border between Thailand and Cambodia, was 9% (29/314). The prevalence of Theileria spp. in meat cattle (6%), buffaloes(6%), meat goats (6%) and crossbred meat sheep (6%).  It was also found that the prevalence of Theileria spp. was linked to neither species nor gender (p>0.05). Polymerase Chain Reaction was used to analyse Theileria spp. since it is very sensitive, specific and fast. Our analysis can be used to prevent and control the spread of Theileria infection in ruminants. Furthermore, this information may guide implemention of a policy on animal movement within the border regions

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How to Cite
Kaewhom, P., & Srikijkasemwat, K. (2022). A molecular survey of Theileria spp. in Ruminants in the Thailand-Cambodia border region. International Journal of Agricultural Technology, 18(1), 205–214. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6608
Issue
Vol. 18 No. 1 (2022): January
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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