Potential of PGPR isolated from rhizosphere of pulpwood trees in stimulating the growth of Eucalyptus pellita F. Muell
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Abstract
Eucalyptus pellita is an alternative plant for Acacia crassicarpa and Acacia mangium as raw materials for pulp and paper. E. pellita is a plant that easily adapts to various environmental conditions, but its growth rate is lower than that of A. crassicarpa and A. mangium. The growth rate of E. pellita can be stimulated by applying plant growth-promoting rhizobacteria (PGPR). A study was conducted to characterize and analyze the ability of 25 PGPR isolates that isolated from rhizosphere of E. pellita seedlings in stimulating the growth of E. pellita. Characteristics of PGPR were analyzed consisted of the isolates’ ability to produce IAA, siderophores, phosphate solubilization, ability as a biocontrol, hypersensitivity test using tobacco plants, and compatibility test between isolates. Stimulating the growth of E. pellita seedlings was carried out with the following treatments: inoculation of a consortium of five selected isolates with or without the addition of fungicides. As a comparison, E. pellita seedlings were also treated without inoculation of a PGPR consortium, and some seedlings were added with fungicides, as well as fungicides and fertilizer. Five isolates were selected which were applied for stimulating the growth of E. pellita. The selected isolates were known to produce IAA, siderophores, phosphate solubilization, and be able to inhibit the growth of Ralstonia solanacearum or Cylindroclaium sp.. In addition, the isolates were not pathogenic, because they did not cause necrotic on tobacco plants. The five isolates were employed as a consortium because the isolates were mutually compatible. The most effective treatment in stimulating the growth of E. pellita was the inoculation of PGPR consortium with the addition of fungicide which caused an increase in plant height of 46.92% and stem diameter of 22.88% compared to control. In contrast, treatment with the addition of fungicides actually inhibits the growth of E. pellita.
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