Efficacy of culture filtrate from Fusarium oxysporum F221-B against plant pathogenic fungi in vitro and Fusarium root rot and wilt disease in hydroponics
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Abstract
The purpose of this research was to evaluate the potential of culture filtrate (CF) of non-pathogenic Fusarium oxysporum (F221-B) against 10 plant pathogenic fungi namely, Curvularia sp. (C11, C12), F. semitectum (F113), F. oxysporum f.sp. lactucae (F221-R, F422-G), Rhizoctonia spp. (R111, R112, R113) and R. solani (R11, R12) in vitro and Fusarium root rot disease in hydroponics. In vitro antifungal activity analysis indicated that all test concentrations of F221-B culture filtrate could slightly inhibit mycelial growth of all tested fungal pathogens less than 39 % over control. Interestingly, the CF at all test concentrations revealed the greatest spore germination inhibition 100% over control against F113, F221-R and F422-G whereas spore germinations of C11 and C12 were completely inhibited by the CF at the concentrations of 60, 80 and 100%. In addition, spore abnormalities such as swelling and lysis were noted. Regard to antifungal analysis in hydroponics, the most striking and remarkable result was obtained. The non-pathogenic F. oxysporum F221-B was proved to be efficient in reducing disease severity of Fusariumroot rot in lettuce more than 90% and producing significantly better growth than that in non-treated lettuces. Moreover, no significant degree of difference in terms of lettuce growth was noted between using culture filtrate and spore suspension of F221-B.
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