Genetic relationships of Myrothecium roridum isolated from water hyacinth in Thailand using ISSR markers and ITS sequence analysis
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Abstract
Water hyacinth (Eichhornia crassipes) is an invasive aquatic weed causing serious threats to irrigation networks and drainage canals in many countries including Thailand. At present, the management focus for this weed has changed from pesticides to biological control using natural enemies of water hyacinth including fungal pathogens. The leaf blight diseased water hyacinths were collected from 19 provinces of Thailand. One hundred thirty-seven fungal isolates were observed for morphological characteristics. According to morphological and molecular data sequenced in the internal transcribed sequence (ITS) region of the rDNA, the fungal isolates were identified as Myrothecium roridum. Inter simple sequence repeat (ISSR) markers were used to evaluate genetic variation of forty-two isolates of M. roridum. The results showed that forty-one polymorphic bands were generated using three ISSR primers. The cluster analysis indicated that M. roridum isolates could be separated into 6 subgroups at cophenetic values (r) = 0.99, supported by a 100% bootstrap value. There was no a correlation between locations and the genetic diversity of M. roridum.
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