Two new bottle gourd fruit rot causing pathogens from Sub-Himalayan West Bengal
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Abstract
Colletotrichum sp.and Fusarium sp. are the two major fungi that cause bottle gourd fruit rot. Typical fruit symptoms include hard, sunken, and dark brown to black patches gradually covering entirely the whole fruit. Pathogen isolates were obtained from diseased bottle gourd fruits, on PDA medium forming a white to gray colonies. The conidia of Colletotrichum sp. were hyaline in color, cylindrical with both apices rounded. On the other hand the conidia of Fusarium sp. were also hyaline, beaked and having transverse septa. Pathogenicity tests with representative isolates were conducted on symptomless fresh bottle gourd fruits. Both the tested fungal pathogen caused fruit rot on bottle gourd fruit. Koch’s postulates were fulfilled by reisolation of the pathogens from the test fruits. PCR analysis (using universal primer set ITS1/ ITS4) of genomic DNA from the pathogens and bioinformatic analysis further confirmed presence of the pathogens. These two fungi as fruit rot causing pathogens in bottle gourd are being reported for the first time.
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