Biocontrol of virulent Ralstonia solanacearum isolates by an indigenous Bacillus cereus

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Published: Jan 20, 2017
Keywords:
Ralstonia solanacearum Wilt Tomato Bacillus cereus Antagonism

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Mandal H.
Department of Botany, University of North Bengal, Siliguri- 734013 India
Chakraborty, P. S.
Department of Botany, University of North Bengal, Siliguri- 734013 India
Saha, D. A.
Department of Botany, University of North Bengal, Siliguri- 734013 India
Sarkar, T.
Department of Botany, University of North Bengal, Siliguri- 734013 India
Saha, D.
Department of Biotechnology, University of North Bengal, Siliguri- 734013 India
Saha, A.
Department of Botany, University of North Bengal, Siliguri- 734013 India

Abstract

In Sub-Himalayan West Bengal tomato is grown round the year. Substantial loss of the crop has been experienced by the farmers’ of the area due to wilt disease caused by Ralstonia solanacearum. R. solanacearum is a devastating, soil borne bacterial pathogen of tomato. The pathogen is non-motile in plant but highly motile in culture. In the present study sixteen strains of R. solanacearum were isolated, purified and identified on the basis of physiological and biochemical characteristics. Pathogenicity of all the sixteen isolates was tested and all the isolates were found to be pathogenic.Three of them were virulent and coded as RSG01, RSG02 and RSG03. Identification of most virulent bacterium (RSG01) was also done by 16S rRNA studies. Finally the most virulent pathogen was controlled by an indigenous antagonistic soil bacteria Bacillus cereus.

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Mandal H., Chakraborty, P. S., Saha, D. A., Sarkar, T., Saha, D., & Saha, A. (2017). Biocontrol of virulent Ralstonia solanacearum isolates by an indigenous Bacillus cereus. International Journal of Agricultural Technology, 13(1), 19–30. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6708
Issue
Vol. 13 No. 1 (2017): January
Section
Original Study
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