Controlling lettuce leaf spot disease using antagonistic yeasts
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Abstract
The experiment was investigated the antagonistic yeasts to potential against leaf spot in lettuce disease by Cercospora lactucae-sativae. Use isolation of epiphytic yeasts was made from surfaces of healthy lettuce 12 isolate and vegetable and fruits 63 isolates were obtained. Preliminary test showed that 24 isolates gives percentage of growth inhibition higher than 30. Then 24 isolates were selected to do efficacy test on Cercospora lactucae-sativae using dual culture method. Results on percentage of growth inhibition are as 14.75 to 77.18. Fifth yeast isolates (CMY057, CMY050, CMY071, CMY027 and CMY035) were selected for their inhibitory effects on conidial and assayed by spore germination for their antagonistic. Then 5 isolates of higher inhibition were selected to test spore germination on agar to show inhibitory effect against Cercospora lactucae-sativae, the plant pathogen causing leaf spot disease on lettuce. Results Four yeast isolates (CMY050, CMY071, CMY027 and CMY035) the conidial germinate on inhibition at 12 hours.
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