Controlling anthracnose of Passion fruit by antagonistic yeast
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Abstract
The purpose of this study was to survey, isolate and collect of the yeast fungi from fruits and vegetable crops. The collected yeasts are used for controlling Colletotrichum boninense a causal agent of passion fruit anthracnose, in the laboratory and in the greenhouse. Seventeen isolates of C. boninense are isolated from various locations of passion fruit farms in Chiang Mai. Of these three isolate are carbendazim resistance. The results shown that 49 isolates of the yeasts are isolated from 28 samples of fruits and vegetable crops. Using dual culture agar plating technique for screening the antagonistic activity against the growth of C. boninense which resistance to carbendazim found that 10 isolate of the yeasts are able to inhibit the growth of this fungus, of these, isolate BMF1 give the maximum inhibiting at 67.69 percent. This BMF1 will be used for the further experiment to control the passion fruit anthracnose in the greenhouse. C. boninense was first described from Crinum asiaticum var. sinicum (Amaryllidaceae) collected in the Bonin Islands, Japan (Moriwaki et al. 2003). According to these authors, the species was associated with a variety of host plants in Japan, including Clivia miniata (Amaryllidaceae), Cucumis melo (Cucurbitaceae), Cattleya sp., Cymbidium sp. and Dendrobium kingianum (Orchidaceae), Passiflora edulis (Passifloraceae) and Prunus mume (Rosaceae).
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