Mycelial growth, antioxidant and antibacterial properties of Boletus griseipurpureus from south Thailand
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Abstract
Boletus griseipurpureus Corner is a popular edible ectomycorrhizal mushroom in the south of Thailand. The objective of this study was to identify optimal media for mycelial formation, antioxidant and antimicrobial activities. Of the four media tested, PDA, Boletus medium, MMN, and MMN with 10 % fresh basidiocarp hot water extract, the latter medium was superior for mycelial dry weight increment. Methanolic extraction of dried basidiocarps showed that the DPPH radical scavenging activity (200.3+4.4 µg vitamin C/g sample) was stronger than crude ethyl acetate extract (77.9+0.4 µg vitamin C/g sample). Ferric reducing antioxidant power (FRAP) gave similar results. Crude basidiocarp extracts inhibited Pseudomonas aerunginosa TISTR 2370 (MIC = 0.39 mg/ml) and Enterococcus faecalis TISTR 379 (MIC = 0.78 mg/ml) in agar well diffusion tests.
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References
Alves, M. J., Ferreira, I. C. F. R., Martins, A. and Pintado, M. (2012). Antimicrobial activity of wild mushroom extracts against clinical isolates resistant to different antibiotics. Journal of Applied Microbiology 113:466-475.
Aung-aud-chariya, A., Bangrak, P., Lumyong, S. Worrapong, P., Aggangan, N. S. and Kamlangdee, N. (2015). RNA polymerase II secondary largest molecular identification of Boletus griseipurpureus Corner from Thailand and antibacterial activity of basidiocarp extracts. Jundishaphur Journal of Microbiology 8: e15552.
Aung-aud-chariya, A., Bangrak, P., Dell, B., Lumyong, S. and Kamlangdee, N. (2012). Preliminary molecular identification of Boletus griseipurpureus Corner from Thailand and its nutritional value. Journal of Agricultural technology 8: 1989-1996.
Benzie, I. F. F. and Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of Antioxidant Power: The Frap Assay. Analytical Biochemistry 239: 70-76.
Cai, M., Petenella, D. and Vidale, E. (2010). Income generation from wild mushrooms in marginal rural areas. Forest policy and economics. Forest Policy and Economics13: 221-226.
Chang, Y. S. and Lee, S. S. (2004). Utilization of macrofungi species in Malaysia. Fungal Diversity15:15-22.
Cheung, P. C. K. (2010). The nutritional and health benefit of mushroom. Nutrition Bulletin 35: 292-299.
Cotelle, N., Bemier, J. L., Catteau, J. P., Pommery, J., Wallet, J. C. and Gaydou, E. M. (1996). Antioxidant properties of hydroxyl-flavones. Free Radical Biology and Medicine 20: 35-43.
De Araujo, A. A. and Roussos, S. (2002). A technique for mycelial development of ectomycorrhizal fungi on agar media. Applied Biochemistry and Biotechnology 98-100: 311-318.
Dogon, H. H. and Aydim, A. (2013). Determination of Antimicrobial effect, antioxidant activity and phenolic contents of dessert truffle in Turkey. African Journal of Traditional, Complementary and Alternative Medicines 10:52-58.
Gomez, K. A. and Gomez, A. A. (1984). Statistical procedures for agricultural research. Second edition. John Wiley and Sons, NewYork. 680 pp.
Hamza A., Zouari, N., Zouari, S., Jdir, H., Zaidi, S., Gtari, M. and Neffati, M. (2016). Nutraceutical potential, antioxidant and antibacterial activities of Terfezia boudieri Chatin, a wild edible dessert truffle from Tunisia arid zone. Arabian Journal of Chemistry 9:383-389.
Hatakeyama, T. and Ohmasa, M. (2004). Mycelial growth characteristic in a split and plate culture of four strains of the genus Suillus. Mycoscience 45:188-199.
Ishikawa, Y., Morimoto, K. and Hamasaki, T. (1984). Flavoglaucin, a metabolite of Eurotium chevalieri, its antioxidation and synergism with tocopherol. Journal of American Oil Chemists’ Society 61:1864-1868.
Islam, F. and Ohga, S. (2013). Effects of media formulation on the growth and morphology of ectomycorrhizae and their association with host plant. Agronomy 1-12.
Kibar, B. and Peksen, A. (2011). Nutritional and environmental requirement of vegetative growth for edible ectomycorrhizal mushroom Tricholoma terreum. Agriculture 98:409-411.
Kim, C. S., Moon, E., Choi, S. U., Kim, S. Y., Lee, K. R. and Kim, K. H. (2015). Cytotoxic and anti-inflammatory disulfide compounds from the fruiting bodies of Boletus pseudocalopus. The Journal of Antibiotics 68:414-416.
Kumla, J., Danell, E., Bussaban B. and Lumyong, S. (2011). Suitable growth conditions and nutrient factors on in vitro culture of Phlebopus portentosus (Boletales). Chiang Mai Journal Science 38:156-159.
Lau, M. F. (2014). Identification and factors that affecting the growth of the indigenous mushroom, Boletus sp. in Bachok Kelantan, Malaysia. (Master ‘s thesis). Faculty of Science, Universiti Sains Malaysia.
Muniandy, S., Daud, F., Senafi, S., Noor, M. N., Kumaran, M., Alwi, A. N. A. M. et al. (2016). Active compound, antiproliferative and effect on stz induced zebrafish of various crude extracts from Boletus qriseipurpureus. Malaysian Applied Biology 45:69-80.
Ogidi, O. C., Oyetaya, V. C. and Akinyele, B. J. (2015). In vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, Lenzites quercina. International Journal of Microbiology.
Ohta, A. and Fujiwara, N. (2003). Fruit-body production of an ectomycorrhizal fungus in genus Boletus in pure culture. Mycoscience 44: 95-300.
Rossi, M. J. and Oliveira, V. L. (2011). Growth of ectomycorrhizal fungus Pisolithus microcarpus in different nutritional conditions. Brazilian Journal of Microbiology 42: 624-632.
Sanmee, R., Dell, B., Lumyong, L., Izumori, K. and Lumyong, S. (2003). Nutritive value of popular wild edible mushroom from northern Thailand. Food Chemistry 82:527-532.
Sarker, S. D., Nahar, L. and Kumarasamy, K. (2007). Microtitre plate -based antibacterial assay incorporating resazurin as an indicator of cell growth, and application in the in vitro antibacterial screening of phytochemicals. Methods 42:321-324.
Tepe, B. D., Daferera, A. Sokmen, M. and Polissiou, M. (2005). Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food chemistry 90:333-340.
Tibuhwa, T. D. (2013). Wild mushroom – an underutilization healthy food resource and income generator: experience from Tanzania rural area. Journal of Ethnobiology and Ethnomedicine 20:9-49.
Velioglu, Y. S., Mazza, G., Gao, L. and Ooma, B. D. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 46:4113-4117.
Venkatachalapathi, A. and Paulsamy, S. (2016). Exploration of wild medicinal mushroom species in Walayar Valley, the Southern Western Ghats of Coimbatore District Tami Nadu. Mycophere 7:118-130.
Xu, M., Zhu, J., Kang, H., Xu, A., Zhang, J. and Li, F. (2008). Optimum condition for pure culture of major ectomycorrhizal fungi obtained from Pinus sylvestris var. mongolica plantation in Southeastern Keerqin sandy lands. China Journal of Forestry Research 19:113-118.
Yuswan, M. H. M. Y., Al-Obaidi, J., Rahayu, A., Sahidan, S., Shazrul, F. and Fauzi, D. (2015). New bioactive molecules with potential antioxidant activity from various extracts of wild edible gelam mushroom (Boletus spp.). Advances in Bioscience and Biotechnology 6:320-329.