Potential use of extracts and active constituent from Desmodium sequax to control fungal plant diseases

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Published: Mar 15, 2022
Keywords:
Desmodium sequax Antifungal activity Lupeol Triterpenoid Plant diseases

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Do, T. H. T.
Research and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry, Hanoi, Vietnam
Pham, T. H.
Center for High Technology Development, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Pham, G. V.
Institute for Tropical Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Vo, K. A.
Institute for Tropical Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Nguyen, T. T. T.
Institute for Tropical Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Vu, D. H.
Department of Pharmaceutical Chemistry and Pesticides Technology, School of Chemical Engineering, Hanoi University of Science and Technology, Hanoi, Vietnam
Nguyen, X. C.
Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, Vietnam
Vu, V. H.
Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Nghiem, D. T.
Hanoi University of Pharmacy, Hanoi, Vietnam
Choi, G. J.
Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea
Nguyen Ngoc, H.
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Nguyen, H. T.
Faculty of Pharmacy, Phenikaa University, Hanoi, Vietnam
Trinh, X. H.
Plant Protection Research Institute, Vietnam Academy of Agricultural Sciences, Hanoi, Vietnam
Le Dang, Q.
Research and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry, Hanoi, Vietnam; Institute for Tropical Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

Abstract

The methanol extract of the whole plant of Desmodium sequax was found to suppress efficiently fungal plant diseases. Especially, it remarkably controlled rice blast (RCB), tomato grey mold (TGM), and red pepper anthracnose (PAN) in vivo. Out of separated fractions, Hex-soluble fraction showed potent control values against RCB (93.75%), TGM (87.5%), wheat leaf rust (WLR) (80%) and PAN (95%) at 3000 µg/mL, respectively. Through bioassay-guided fractionation, compound 1 was isolated from the Hex-soluble fraction and this compound was identified as lupeol on the basis of NMR and ESI-MS data analysis. The in vivo and in vitro antifungal activity of 1 was evaluated against various fungal phytopathogens. Lupeol displayed a moderate inhibition against the mycelial growths of Rhizoctonia solani, Colletotrichum orbiculare, and Magnaporthe oryzae in vitro. Besides, in vivo antifungal efficacy of 1 against TGM and tomato late blight (TLB) over the concentration range of 125–500 µg/mL was described for the first time. The content of lupeol (2.94%) in Hex-soluble fraction was quantified by HPLC analysis. Our study demonstrated that D. sequax is a promising plant resource, contains lupeol as an antifungal constituent, and could be used to control fungal plant pathogens.

Article Details

How to Cite
Do, T. H. T., Pham, T. H., Pham, G. V., Vo, K. A., Nguyen, T. T. T., Vu, D. H., Nguyen, X. C., Vu, V. H., Nghiem, D. T., Choi, G. J., Nguyen Ngoc, H., Nguyen, H. T., Trinh, X. H., & Le Dang, Q. (2022). Potential use of extracts and active constituent from Desmodium sequax to control fungal plant diseases. International Journal of Agricultural Technology, 18(2), 489–502. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6901
Issue
Vol. 18 No. 2 (2022): March
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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