Productivity of five entomopathogenic nematodes in Galleria mellonella L. and their persistence in soil under laboratory conditions

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Published: Mar 15, 2022
Keywords:
Biological control agent Heterorhabditis Infective juvenile Steinernema Soil persistence

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Noitubtim, P.
Institute of Weed Science, Entomology and Plant Pathology College of Agriculture and Food Science, UP Los Baños, College, Laguna 4031, Philippines; Nakhon Si Thammarat Provincial Agricultural Extension Office, Department of Agricultural Extension, Mueang Nakhon Si Thammarat, Nakhon Si Thammarat 80000, Thailand
Caoili, B. L.
Institute of Weed Science, Entomology and Plant Pathology College of Agriculture and Food Science, UP Los Baños, College, Laguna 4031, Philippines
Noosidum, A.
Department of Entomology, Faculty of Agriculture, Kasetsart University, Chatuchak, Bangkok 10900, Thailand

Abstract

The persistence and reproductive ability of entomopathogenic nematodes (EPN) are important factors to consider in insect biological control agents. Therefore, the present study compared the persistence and productivity of five EPN isolates namely, Steinernema glaseri, S. siamkayai, S. carpocapsae All, Heterorhabditis bacteriophora and H. indica EPNKU80 in Galleria mellonella larvae. The productivity among the EPN significantly varied among the nematode species. Generally, Heterorhabditis species produced more infective juveniles (IJ) than Steinernema species in the cadaver. The IJ production was highest in H. bacteriophora but was not statistically different from H. indica (EPNKU80). In contrast, the lowest number of IJ was produced by S. carpocapsae All. The persistence test showed that H. bacteriophora had the highest penetration ability and had the greatest number of EPN produce inside G. mellonella larvae at all exposure times than the other EPN species. In addition, the highest efficacy was observed in H. bacteriophora (84.40%) at 15 day after application, which was statistically different from S. glaseri (63.30%), S. siamkayai (59.10%), H. indica EPNKU80 (58.50%) and S. carpocapsae All (19.30%). It concluded that Heterorhabditis species  was higher soil persistence and IJ reproduction than Steinernema species which used for insect control.

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Noitubtim, P., Caoili, B. L., & Noosidum, A. (2022). Productivity of five entomopathogenic nematodes in Galleria mellonella L. and their persistence in soil under laboratory conditions. International Journal of Agricultural Technology, 18(2), 667–678. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6936
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Vol. 18 No. 2 (2022): March
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Abd - Elgawad, M. M. M. (2017). Status of entomopathogenic nematodes in integrated pest management strategies in Egypt. In: Abd-Elgawad, M. M. M., Askary, T. H. and Coupland, J. (eds) Biocontrol Agents: Entomopathogenic and Slug Parasitic Nematodes. Wallingford, CAB International, UK, pp. 473-501.

Akhurst, R., and Smith, K. (2002). Regulation and safety. In R. Gaugler (Ed.), Entomopathogenic Nematology (pp. 311–332). Wallingford, UK: CABI Publishing.

Baur, M. E. and Kaya, H. K. (2001). Persistence of entomopathogenic nematodes. In: Baur, M. E. and Fuxa, J. R. (Eds.), Factors affecting the survival of entomopathogens. Southern Cooperative Series Bulletin 400.

Bedding, R. A., Akhurst, R. J. and Kaya, H. K. (1993). Nematodes and the Biological Control of Insect Pests. CSIRO Publications, East Melbourne, Victoria. 178 p.

Boemare, N. E., Laumond, C. and Mauleon, H. (1996). The entomopathogenic nematode bacterium complex: biology, life cycle and vertebrate safety. Biocontrol Sci. Technol. 6: 333-345.

Curran, J. (1993). Post-application biology of entomopathogenic nematodes in soil, pp. 67-78. In Bedding, R.A., Akhurst, R. and Kaya, H. K. (eds). Nematodes and the Biological Control of Insect pests. CSIRO Publications, East Melbourne, Australia.

Fateh, F. S., Mukhtar, T., Kazmi, M. R., Abbassi, N. A. and Arif, A. M. (2017). Prevalence of citrus decline in district Sargodha. Pakistan Journal of Agricultural Sciences, 54:9-13.

Gaugler, R. and Han, R. (2002). Production technology. In: Entomopathogenic nematology (ed. R. Gaugler). CABI Wallingford, UK, pp.289-310.

Glazer, I., Koltai, H., Zioni, C. N. S. and Segal, D. (1994). Life cycle and reproduction in Heterorhabditis. In: Genetics of entomopathogenic nematodes bacterium complex (eds. A.M. Burnell, R.U. Ehlers and J.P. Masson). pp.80-89.

Han, R., Pang, X. and Li, L. (1995). Optimization of the medium components for the solid culture of entomopathogenic Steinernema and Heterorhabditis nematodes. Natural Enemies of Insects, 17:153-164.

Han, R., Li, L. and Pang, X. (1997). Modeling of the culture parameters for production of Steinernema carpocapsae and Heterorhabditis bacteriophora in solid culture. Natural Enemies of Insects, 19:75-83.

Hussian, M. A., Mukhtar, T. and Kayani, M. Z. (2016). Reproduction of Meloidogyne incognita on resistant and susceptible okra cultivars. Pakistan Journal of Agricultural Sciences, 53: 371-375.

Jansson, R. K., Lecrone, S. H. and Gaugler, R. (1991). Comparison of single and multiple releases of Heterorhabditis bacteriophora Poinar (Nematoda:Heterorhabditidae) for control of Cylas formicarius (F.) (Coleoptera: Apionidae). Biological control, 1:320-328.

Kassi, A. K., Javed, H. and Mukhtar, T. (2018). Screening of okra varieties for resistance against Helicoverpa armigera. Pakistan Journal of Zoology, 50:91-95.

Kaya, H. K. and Gaugler, R. (1993). Entomopathogenic nematodes. Annual Review of Entomology, 38:181-206.

Kaya, H. K. (1985). Entomogenous nematodes for insect control in IPM systems. In: Biological control in agricultural IPM systems (eds. M.A. Hoy and D.C. Herzog). Academic Press, New York, pp.283-302.

Kaya, H. K. (1987). Diseases caused by nematodes. In: Epizootiology of insect diseases (eds. J.R. Fuxa and Y. Tanada). J. Wiley, New York, pp.453-470.

Kaya, H. K., Bedding, R. A. and Akhurst, R. J. (1993). An overview of insect-parasitic and entomopathogenic nematodes. In: Nematodes and the biological control of insect pests (Eds.) Bedding R, Akhurst R, Kaya H, East Melbourne, Australia: CSIRO Publications, pp.1-10.

Kaya, H. K. and Stock, S. P. (1997). Techniques in insect nematology. In: Lacey LA (eds) Manual of techniques in insect pathology. Academic Press, San Diego, pp 282–324.

Kondo, E. and Ishibashi, N. (1987). Comparative infectivity and development of the entomopathogenic nematodes Steinernema spp. on the lepidopterous insect larvae, Spodoptera litura (Noctuidae) and Galleria mellonella (Galleridae). Jpn. Journal of Nematology, 17:35-41.

Kung, S., Gaugler, R. and Kaya, H. K. (1991). Effects of soil temperature, moisture, and relative humidity on entomopathogenic nematode persistence. Journal of Invertebrate Pathology, 57:242-249.

Mannion, C. M. and Jansson, R. K. (1992). Comparison of ten entomopathogenic nematodes for biological control of the sweet potato weevil (Coleoptera: Apionidae). Journal of Economic Entomology, 85:1642-1650.

Mannion, C. M. (1992). Selection of suitable entomopathogenic nematodes for biological control of Cylas formicarius (Coleoptera: Apionidae). (Ph.D. thesis), University of Florida, Gainesville, FL.

Mohamed, M. A. and Coppel, H. C. (1983). Mass rearing of the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae), for small-scale laboratory studies. The great lakes Entomologist, 16:139-141.

Molyneux, R. S. (1985). Survival of infective juveniles of Heterorhabditis spp., and Steinernema spp. at various temperatures and their subsequent infectivity for insects. Revue de Nematologie, 8:165-170.

Morris, O. N., Converse, V. and Harding, J. (1990). Virulence of entomopathogenic nematode-bacteria complex for larvae of noctuids, a geometrid, and a pyralid. Canadian Entomologist, 122: 309-319. https://doi. org/10.4039/Ent122309-3

Nabeel, M., Javed, H. and Mukhtar, T. (2018). Occurrence of Chilo partellus on maize in major maize growing areas of Punjab, Pakistan. Pakistan Journal of Zoology, 50: 317-323. http://dx.doi.org/10.17582/ journal.pjz/2018.50.1.317.323

Poinar, Jr., G. O. (1990). Taxonomy and biology of Steinernematidae and Heterorhabditidae, pp.75-90. In R. Gaugler and H.K. Kaya, eds., Entomopathogenic Nematodes in Biological Control. CRC Press, Boca Raton, FL.

Rahoo, A. M., Mukhtar, T., Abro, S. I., Gowen, S. R. and Bughio, B. A. (2016a). Effect of temperature on emergence of Steinernema feltiae from infected Galleria mellonella cadavers under moist and dry conditions. Pakistan journal of nematology, 34:171-176. https:// doi.org/10.18681 /pjn. v34.i02.p17

Rahoo, A. M., Mukhtar, T., Bughio, B. A., Gowen, S. R. and Rahoo, R. K. (2017b). Infection of Galleria mellonella larvae by Steinernema affine and production of infective juveniles. Pakistan journal of nematology, 35:65-71.

Rahoo, A. M., Mukhtar, T., Gowen, S. R., Pembroke, B. and Rahu, M. A. (2016b). Emergence of Steinernema feltiae from infected Galleria mellonella cadavers in moist and dry conditions. Pakistan journal of nematology, 34:81-86.

San-Blas, E., Gowen, S. R. and Pembroke, B. (2008). Steinernema feltiae: Ammonia triggers the emergence of their infective juveniles. Experimental Parasitology, 119:180-185.

Shapiro-Ilan, D. I., Gouge, D. H. and Koppenho¨fer, A. M. (2002a). Factors affecting commercial success: Case studies in cotton, turf and citrus. pp.333-356 in R. Gaugler, ed. Entomopathogenic Nematology. Wallingford, UK: CABI Publishing.

Shapiro-Ilan, D. I., Gaugler, R., Tedders, W. L., Brown, I. and Lewis, E. E. (2002b). Optimization of inoculation for in vivo production of entomopathogenic nematodes. Journal of Nematology, 34:343-350.

Shapiro-Ilan, D. I., Stuart, R. J. and McCoy, C. W. (2006). A comparison of entomopathogenic nematode longevity in soil under laboratory conditions. Journal of Nematology, 38:119-129.

Somsook, V.., Tantichodok, A. and Katenud, U. (1986). Control of Cossus sp. with the entomopathogenic nematode Steinernema carpocapsae. Entomological Zoology Gazine, 8:115-119.

Stock, S. P. (2005). Morphology and systematics of nematodes used in biocontrol. In: Grewal PS, Ehlers R-U, Shapiro-Ilan, D.I., editors. Nematodes as biocontrol agents. New York: CABI; pp.3-43.

Stock, S. P., Choo, H. Y. and Kaya, H. K. (1998). An entomopathogenic nematode, Steinernema monticolum sp.n. (Rhabditida: Steinemematidae). Nematologica, 43: 15-30.

Stuart, R. J. and Gaugler, R. (1994). Patchiness in populations of entomopathogenic nematodes. Journal of Invertebrate Pathology, 64:39-45.

Tanada, Y. and Fuxa, J. R., (1989). The pathogen population. In: Epizootiology of insect diseases (eds. J.R. Fuxa and Y. Tanada). J. Wiley, New York, pp.113-157.

White, G. F. (1927). A method for obtaining infective juveniles from cultures. Science, 66: 302-303.

Womersly, C. H. (1990). Dehydration survival and anhydrobiotic potential, pp.117-137 In Gaugler, R. and Kaya, H. K. (eds.) Entomopathogenic Nematodes in Biological control. CRC Press, Inc., Boca Raton, Florida.

Wouts, W. M. (1984). The biology, life cycle and redescription of Neoaplectana bibionis Bovien, 1937 (Nematoda: Steinernematidae). J. Nematol., 12:62-72.

Zioni, S., Glazer, I. and Segal, D. (1992). Life cycle and reproductive potential of the nematodes Heterorhabditis bacteriophora strain HP 88. Journal of Nematology, 24:352-358.