Fungal flora of mechanic workshops and its bioremediation
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Abstract
The study was carried out to isolate and identify the fungi species present in contaminated soil samples from various mechanic workshops and also evaluate their biodegradation potential. Soil samples were obtained from three locations and one gram of each was added to nine milliliter of distilled water to give a tenfold serial dilution of which was made up to 10-3 dilution. Zero point one milliliter aliquot of 10-3 of each sample was pour-plated on prepared Sabouraud Dextrose Agar (SDA) and incubated at 250C for 5 days for isolation of the heterotrophic and hydrocarbonclastic fungi. The fungal species isolated were seven namely; Aspergillus niger, Aspergillus terreus, Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae, Fusarium solani and Penicillium chrysogenum. The fungal counts ranged from 1.6 x 10-4 to 5.1 x 10-4(cfu/ml) There was variation in the morphological and microscopic characteristics of the seven fungal isolates. The percentage occurrence amongst the fungal isolates was higher in Aspergillus niger (41.17%) followed by Aspergillus terreus(17.64%), Aspergillus fumigatus(17.64%), Fusarium solani (5.88%), Saccharomyces cerevisiae (5.88%), Candida albicans(5.88%), and Penicilluim chrysogenum(5.88%). The biodegradation potential of the fungal isolates were confirmed using used engine oil as sole source carbon and energy through the vapour phase transfer method and Aspergillus niger was demonstrated as the most versatile among other isolates, making it a promising candidate for bioremediation of soil polluted with petroleum hydrocarbons. Thus, these oil-degrading microbes are abundant in soil and can be applied for bioremediation of soils contaminated with petroleum and petroleum products
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