Nanofibers derived from endophytic Chaetomium brasilense for growth stimulation of rice
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Abstract
Chaetomiumn brasilense PT302 was endophytically isolated from rice var Supanburi 1 and Prathumthani 80 which firstly selected for investigation to promote the growth of rice. It is morphologically and molecular phylogenetic confirmation through ITS1 and ITS4 as Ch.brasilense PT302 compared to Achaetomium strumarium (JX863914) as out group. Crude metabolites (crude hexane, crude ethyl acetate and crude methanol) at 50 ppm from Ch. brasiliense were proved to promote seed germination of rice var. Supanburi 1 and Pathumtani 80 at 7 days which found significantly higher seed germination than the non-treated control. Nanofibers constructed Ch. brasiliense promoted significantly higher seed germination, plant height and number of tillers than the non-treated control in rice var Supanburi 1 and Prathumthani 80 in 7 days at low concentration of 5 ppm planted in Chachengsao and Bangkok soil series. Nanofibers derived from endophytic Ch. brasilense is actively promoted plant growth at lower concentration than crude metabolites. It is firstly reported the natural product nanofiber constructed from endophytic Ch. brasilense to stimulate plant growth
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References
Abbott, S. P., Sigler, L., McAleer, R., McGough, D. A., Rinaldi, M. G. and Mizell, G. (1995). Fatal cerebral mycoses caused by the Ascomycete Chaetomium strumarium. Journal of Clinical Microbiology, 33:2692-2698.
Ahammed, S. K., Aggarwal, R. and Renu (2005). Use of PCR based RAPD technique for characterization of Chaetomium globosum isolates. Acta Phytopathologica et Entomologica Hungarica, 40:303-314.
Basra, S. M. A., Farooq, M., Wahid, A. and Khan, M. B. (2006). Rice seed invigoration by hormonal and vitamin priming. Seed Science and Technology, 34:753-758.
Basu, R. N. and Pal, P. (1979). Physiochemical control of seed deterioration in rice. The Indian Journal of Agricultural Sciences, 49:1-6.
Berjak, P. and Villiers, T. A. (1972). Aging in plant embryos II. Age-induced damage and its repair during early germination. New Phytologist, 71:135-145.
Bomke, C., Rojas, M. C., Gong, F., Hedden P. and Tudzynski, B. (2008). Isolation and characterization of the gibberellin biosynthetic gene cluster in Sphaceloma manihoticola. Applied and Environmental Microbiology, 74:5325-5339.
Carvalho, B. M. L., dos Santos Dias, D. C. F., dos Santos Dias, L. A. and Araujo, E. F. (2005). Germination and vigour of primed asparagus seeds. Science of Agriculture, 62:319324.
Chandra, Sekhar, V., Prameela Devi, T., Kamil, D. and Dama Ram, C. (2015). Division of Plant Pathology Indian. Agricultural Research Institute, Pusa Campus, New Delhi, New Delhi, India.
Dar, Joselito and Soytong, K. (2013). In vitro testing of nanomaterials containing ethyl acetate extract from Chaetomium globosum against Fusarium orysparum f sp. lycopersici (race 2). International Journal of Agricultural Technology, 5:28-29.
Domsch, K. M., Gams, W. and Anderson, T. H. (1993). Compendium of soil fungi vol. I & 2nd Ed. Academic Press, London, 859:405.
Elibol, O. H., Morisette, D. D., Denton, J. P. and Bashir, R. (2003). Integrated nanoscale silicon sensors using top-down fabrication. Applied Physics Letters, 83:4613-4615.
Ellis, M. B. (1971). Dematiaceous Hyphomycetes. Commonwealth Mycological Institue, Kews. 608 p. Gochenaur, A. E. and Backus, M.P. 1962. A new species of Neurospora from Wisconsin lowland soil. Mycologia, 54:555-562.
Giri, G. S. and Schillinger, W. F. (2003). Seed priming winter wheat for germination, emergence, and yield. Crop Science, 43:2135-2141.
Hamayun, M., Khan, S. A., Khan, A. L., Rehman, G., Kim, Y., Iqbal, I., Hussain, J., Sohn, E. and Lee, I. J. (2010). Gibberellins production and plant growth promotion from pure cultures of Cladosporium sp. MH-6 isolated from Cucumber (Cucumis sativus. L.). Mycologia, 102:989-995.
Hyuncheol, D., Swenson, C. and James B. G. (1998). Chaetochalasin A: A new Bioactive Metabolite from Chaetomium brasiliense. Tetrahedron Letters39, 1998:7633-7636.
Kanokmedhakul, S., Kanokmedhakul, K., Nasomjai, P., Louangsysouphanh, S., Soytong, K., Kongsaeree. P., Prabpai. S. and Suksamrarn, A. (2006). Antifungal azaphilones from the fungus Chaetomium cupreum CC3003. Journal Natural Products, 69:891-895.
Kanokmedhakul, S., Kanokmedhakul, K., Phonkerd, N., Soytong, K., Kongsaree, P. and Suksamrarn, A. (2002). Antimycobacterial anthraquinonechromanone compound and diketopiperazine alkaloid from the fungus Chaetomium globosum KMITL-N0802. Planta Medica, 68:834-836.
Kawaide, H. (2006). Biochemical and molecular analysis of gibberellins biosynthesis in fungi. Bioscience, Biotechnology, and Biochemistry, 70:583-590.
Khan, A. L., Hamayun, M., Kim, Y. H., Kang, S. M., Lee, J. H. and Lee, I. J. (2011). Gibberellins producing endophytic Aspergillus fumigatus sp. LH02 influenced endogenous phytohormonal levels, plant growth and isoflavone biosynthesis in soybean under salt stress. Process Biochemistry, 46:440-447.
Khumkomkat, P., Kanokmedhakul, S., Kanokmedhakul, K., Hahnvajanawong, C. and Soytong, K. (2009). Antimalarial and cytotoxic depsidones from the fungus Chaetomium brasiliense. Journal of Natural Products, 72:1487-1491.
Kumar, S., Stecher, G. and Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33:1870-1874.
Leewijit, T., Pongnak, W., Soytong, K. and Poeaim, S. (2016). Isolation of Soil and Endophytic Fungi from Rice (Oryza sativa L.)International Journal of Agricultural Technology, 12:2191-2202.
Li, H. Q., Li, X. J., Wang, Y. L., Zhang, Q., Zhang, A. L., Gao, J. M. and Zhang, X. C. (2011a). Antifungal metabolites from Chaetomium globosum, an endophytic fungus in Ginkgo biloba. Biochemical Systematics and Ecology, 39:876-879.
Li, X., Xu, H., Chen, Z. S. and Chen, G. (2011b). Biosynthesis of nanoparticles by microorganisms and their applications. Journal of Nanomaterials, 16:270-974.
Nath, R., Sharma, G. D. and Barooah, M. (2015). Plant growth promoting endophytic fungi isolated from tea (Camellia sinensis) shrubs of assum, India. Applied ecology and environmental research. 13:877-891.
Parera, C. A. and Cantiffe, D. J. (1994). Presowing seed priming. Hortica Reviews, 16:109-141.
Qin, J. C., Zhang, Y. M., Gao, J. M., Bai, M. S., Yang, S. X., Laatsch, H. and Zhang, A. L. (2009). Bioactive metabolites produced by Chaetomium globosum, an endophytic fungus isolated from Ginkgo biloba. Bioorganic & Medicinal Chemistry Letters, 19:1572-1575.
Rademacher, W. (1994). Gibberellin formation in microorganisms. Plant Growth Regulation, 15:303-314.
Rai, J. N, Tewari, J. P. and Mukerji, K. G. (1964). Achaetomium, a new genus of Ascomycetes. Canadian Journal of Botany, 42:693-697.
Reddy, V. R., Rao, P. V. and Reddy, C. V. (1988). Chemical composition and nutritive value of processed neem cake. The Indian Journal of Animal Sciences, 68:870-873.
Sibounnavong, P., Sibounnavong, P. S., Kanokmedhakul, S. and Soytong, K. (2012). Antifungal activities of Chaetomium brasilense CB01 and Chaetomium cupreum CC03 against Fusarium oxysporum f.sp. lycopersici race 2. Journal of Agricultural Technology, 8:1029-1038.
Song, J. J., Kanokmedhakul, S., Kanokmedhalkul, K. and Soytong, K. (2018). Application of nano-particles derived from Chaetomium elatum ChE01 to control Pyricularia oryzae causing rice blast. International Journal of Agricultural Technology, 14:923-932.
Song, J. J., Pongnak, W. and Soytong, K. (2016). Antifungal activity of endophytic fungi from palm trees against coffee anthracnose caused by Colletotrichum coffeanum. International Journal of Agricultural Technology 12:527-539.
Song, J. J., Soytong, K. and Kanokmedhakul, S. (2021). Control of rice blast disease caused by Magnaporthe oryzae by application of antifungal nanomaterials from Emericella nidulans. Plant Protection Science, 58. https://doi.org/10.17221/33/2021
Soutter, W. (2012). Nanotechnology in agriculture. AZoNano.com Publishers. Available at http://www.azonano.com/article.aspx?Article ID=3141#1%204.
Soytong, K. (2014). Bio-formulation of Chaetomium cochliodes for controlling brown leaf spot of rice. Journal of Agricultural Technology, 10:321-337.
Soytong, K., Kanokmedhakul, S., Kukongviriyapa, V. and Isobe, M. (2001). Application of Chaetomium species (Ketomium®) as a new broad spectrum biological fungicide for plant disease control: A review article. Fungal Diversity, 7:1-15.
Syamsia, Kuswinantib, T., Syamunb, E. and Masniawati, A. (2015). The potency of endophytic fungal isolates collected from local aromatic rice as indole acetic acid (IAA) producer. Procedia food science, 3:96-103.
Tongon, R. and Soytong, K. (2016). Fungal Metabolites from Chaetomium brasilense to Inhibit Fusarium solani. International Journal of Agricultural Technology, 12:1463-1472.
Umamaheswari, C. and Prabhakaran, N. (2012). Molecular Taxonomy of Chaetomium Species. Division of Plant Pathology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, Delhi India.
Uthandi, S., Karthikeyan, S. and Sabarinathan, K. G. (2010). Gibberellic acid production by Fusarium fuzikoroi SG2. Journal of scientific and industrial research, 69:211-214.
Waqas, M., Khan, A. L., Kamran, M., Hamayun, M., Kang, S. M., Kim, Y. H. and Lee I. J. 2012: Endophytic Fungi Produce Gibberellins and Indoleacetic Acid and Promotes Host-Plant Growth during Stress. Molecules, 17:10754-10773.
White, T. J., Bruns, T., Lee, S. and Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. [ed.], PCR protocols: a guide to methods and applications. New York Academic Press, Inc.: New York. pp.315-322.
Yew, S. M., Chan, C. L., Lee, K.W., Na, S. L., Tan, R. and Hoh, C. C. (2014). A Five-Year Survey of Dematiaceous Fungi in a Tropical Hospital Reveals Potential Opportunistic Species. PLoS ONE, 9:1-10.
Zakria Ahmed, H., Gaber, K., Yaseen, T., Ahmed, Y., El-Gantiry, S. and Helmy, M. (2016). Antagonistic activity of some Chaetomium species against common bean root rot pathogens. Mycology Research and Plant Disease Survey.