Optimization of culture parameters of selenium-enriched Ophiocordyceps sinensis biomass by response surface methodology
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Abstract
The medium composition and culture conditions of 25 mg/L selenium supplementing Ophiocordyceps sinensis was optimized by response surface methodology. The results showed that the optimum medium for Se enrichment of O. sinensis found to be 53.48 g/L saccharose, 11.17 g/L peptone, 1.05 g/L KH2PO4. The biomass and selenium content was 24,93 g/L, 960,31 µg/g, respectively. The obtained optimum conditions for O. sinensis consisted of daylight, temperature 20 °C, initial pH value 6.1. The maximum biomass and total Se yield reached 26,45 g/L, 1068 µg/g, respectively. The result indicated that response surface methodology is a promising method for the optimization of selenium-enriched O. sinensis fermentation process, and which is the basis for further studies of the fungal bioactivity.
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