Antifungal and antibacterial activities of essential oil from Som Keaw (Citrus nobilis) in Thailand
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Abstract
The essential oil from Citrus nobilis leave was displayed antibacterial activity higher than that from the fruit’s peel, with inhibition zone against Erwinia chrysanthemi and Xanthomonas axonopodis at 10,000 ppm as 10.67 mm and 11.33 mm, respectively. The pure essential oil from the peel 20 µL, however, displayed the highest antibacterial activity against E. chrysanthemi and X. axonopodis with the inhibition zone 20.00 mm and 14.00 mm, respectively. The antifungal activity was tested by vapour phase technique against five plant pathogenic fungi Colletotrichum gloeosporioides, Curvularia lunata, Fusarium sacchari, Pythium sp. and Rhizoctonia solani at 5, 10 and 20 µL/disc. Results show the essential oil from the peel extract completely inhibited mycelial growth of Pythium sp. and strongly inhibited R. solani. Additionally, the essential oil from leaf strongly inhibited mycelial growth of Pythium sp. and R. solani. The results suggested the essential oil from leaves and peel of Som Keaw (C. nobilis) serve as a high effective natural deterrent against all of the tested fungi.
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References
Agrios, G. M. (2005). Plant Pathology. 5th edition. New York: Academic Press.
Anonymous (2015). Kasetkaowna “Som Keaw” Bangkhontee. Retrieved from https://web.facebook.com/agriculturemag/photos.
Balouiri, M., Sadili, M. and Ibnsouda, S. K. (2016). Methodes for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6:71-79.
Bernabo, I., Guardia, A., Macirella, R., Sesti, S., Crescente, A. and Brunelli, E. (2016). Effects of long-term exposure to two fungicides, pyrimethanil and tebuconazole, on survival and life history traits of Italian tree Frog (Hylaintermedia). Aquatic Toxicology, 172:56-66.
Blaschek, W., Kasbauer, J., Kraus, J. and Franz, G. (1992). Pythium aphanidermatum: culture, cell wall composition, and isolation and structure of antitumour storage and solubilised cell wall (1----3), (1----6)-beta-D-glucans. Carbohydrate Research, 2:293-307.
Boukhatem, M. N., Ferhat, M. A., Kameli, A., Saidi, F. and Kebir, H. (2014). Lemon grass (Cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan Journal of Medicine, 9:425-431.
Bozkurt, T., Gulnaz, O. and Kacar, Y. A. (2017). Chemical composition of the essential oils from some citrus species and evaluation of the antimicrobial activity. Journal of Environmental Science, Toxicology and Food Technology, 11:2319-2402.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94:223-253.
Cloete, T. E. (2003). Resistance mechanisms of bacteria to antimicrobial compounds. International Journal of Bioderioration and Biodegradation, 51:227-282.
Dharmawan, J., Kasapis, S., Sriramula, P., Lear, M. J. and Curran, P. (2009). Evaluation of aroma active compounds in Pontianak orange peel oil (Citrus nobilis Lour. var. microcarpa Hassk.) by gas chromatography olfactometry, aroma reconstitution and omission test. Journal of Agricultural and Food Chemistry, 57:239-244.
Ferreira, S., Silva, A. M. and Nunes, F. M. (2018). Citrus reticulate Blanco peels as a source of antioxidant and anti-proliferative phenolic compounds. Industrial Crops & Products, 111:141-148.
Gursoy, N., Tepe, B. and Sokmen, M. (2010). Evaluation of chemical composition and antioxidant activity of the peel oil of Citrus nobilis. International Journal of Food Products, 13:983-991.
Hong-Thao, P. T., Mai-Linh, N. V., Hong-Lein, N. T. and Hieu, N. V. (2016). Biological characteristics and antimicrobial activity of endophytic Streptomyces sp. TQR12-4 isolated from Elite Citrus nobilis cultivar Ham Yen of Vietnam. International Journal of Microbiology, 1-7.
Jafari, S., Esfahani, S., Fazeli, M. R., Jamalifar, H., Samadi, M., Samadi, N., Najarian-Toosi, A., Shams, A., Khanavi, M. (2011). Antimicrobial activity of lime essential oil against food-born pathogens isolated from Cream-filled Cakes and Pastries. International Journal of Biological Chemistry, 5:1-8.
Kamal, G. M., Ashraf, M. Y., Hussain, A. I., Shahzadi, A. and Chughtai, M. (2013). Antioxidant potential of peel essential oil of three Pakistani Citrus species: Citrus reticulata, Citrus sinensis and Citrus paradisii. Pakestan Journal of Botany, 45:1449-1454.
Laoongaium, P. (2007). The study antibacterial of essential oil from pomelo peel. Bull Chiang Mai Associated Medical Science, 40:118.
Liu, C., Cheng, Y., Zhang, H., Deng, X., Chen, F. and Xu, J. (2012). Volatile constituents of wild citrus Mangshanyegan (Citrus nobilis Lauriro) peel oil. Journal of Agricultural and Food Chemistry, 60:2617-2628.
Madi, I. S. O. (2017). Fungi associated with grain discoloration (GD) of rice (Oryza sativa var. RD61) and the effeicacy of some plant extracts in suppressing GD causal agents. (Master Thesis). Silpakorn University, Thailand.
Mandalari, G., Bisignano, C., Cirmi, S. and Navarra, M. (2017). Effectiveness of Citrus fruits on Helicobacter pylori. Evidence Based Complementary and Alternative Medicine. 8 p.
Oreke, E. C., Dehne, H. W., Schonbeck, F. and Weber, A. (1994). Crop Production and Crop Protection: Estimated Losses in Major Food and Cash Crops. Elsevier. USA, Amsterdam.
Pakchareon, A. (2005). Selection of some plant for inhibit of Didymella bryoniae, causal agent of gummy stem blight of watermelon. (Bachelor project). Kasetsart University, Thailand.
Panthachod, S., Chongbunyut, J. and Sassa-deepaeng, T. (2009). Essential oil extracts of sweet orange peels (Citrus sinensis (L.) Osbeck). Rajamangala University of Technology Lanna, Chiang Mai, Thailand.
Parveen, S., Wani, A. H., Ganie, A. A., Pala, S. A., Mir, R. A. (2014). Antifungal activity of some plant extracts on some pathogenic fungi. Archives of Phytopathology and Plant Protection, 47:279-284.
Pathirana, H. (2018). Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichtys olivaceus). Fisheries & Aquatic Life, 26:131-139.
Sawbangkhae. (2014). Agricultural Technology. Retrieved from http://info.matichon.co.th/ techno/techno.php?srctag=05049150557&srcday=&search=no.
Songsamoe, S., Matan, N. and Matan, N. (2017). Antifungal activity of Michelia alba oil in the vapor phase and the synergistic effect of major essential oil components against Aspergillus flavus on brown rice. Food Control, 77:150-157.
Vetchakit, A. (2015). Annual report knowledge; Smart products, Samutsongkham province. Retrieved from http://www.samutsongkham.doae.go.th/km/58/1Smart_farmer.pdf.
Vimal, M., Vijaya, P. P., Muntaj, P. and Swwma, M. S. F. (2013). Antibacterial activity of selected compounds of essential oils from indigenous plants. Journal of Chemical and Pharmaceutical Research, 5:248-253.
Worapanit, N., Thabktjakan, W., Petchsuriawong, B., Nopharatana, M. and Vongsawasdi, P. (2014). Characteristics of bitter orange essential oil and its application in jelly. Agricultural Science Journal (Suppl.), 45:369-372.
Wu, F., Jin, Y., Xu, X. and Yang, N. (2017). Electrofluidic pretreatment for enhancing essential oil extraction from citrus fruit peel waste. Journal of Cleaner Production, 159:85-94.
Xiao, Z., Wu, Q., Niu, Y., Wu, M., Zhu, J., Zhou, X., Chen, X., Wang, H., Li, J. and Kong, J. (2017). Characterization of the key aroma compounds in five varieties of mandarins by gas chromatography-olfactometry, odor activity values, aroma recombination and omission analysis. Journal of Agricultural and Food Chemistry, 65:8392-8404.