Scanning electron microscopy and histological analyses of Ficus carica L. cv. Black Jack
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Abstract
A protocol of micropropagation is established for Ficus carica L. cv. Black Jack with excised apical buds from one-year-old mother plant as in vitro explant materials. The response of explants on BAP and 2-iP at different concentrations (0, 10, 20, 30 and 40 µM) were investigated via histological analyses and scanning electron microscopy (SEM). Isodiametric cells and vascular tissues were observed from the cross-sections of apical buds under the treatments of BAP and 2-iP at all concentrations via SEM. Histological analysis confirmed that both cytokinins tested at different concentrations involved the formation of actively dividing meristematic cells prior to differentiate to parenchymatous tissues. It is also proven that more vascular tissues were found at lower epidermal layers. Histological analysis was proved that better response for callus induction was obtained by the adaxial surface on the leaf in contact with the media. SEM analysis confirmed that no structural distortion caused by cytokinins.
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