Biotechnological potential of phosphate-solubilizing Pseudomonas migulae strain GEOT18
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Abstract
The biotechnological potential of phosphate-solubilizing bacterial Pseudomonas migulae strain GEOT18, isolated from the endosphere of Dactylorhiza incarnata (L.) Soó (Orchidaceae Juss) was investigated. The phosphate solubilizing activity of this strain was assessed qualitatively and quantitatively by their ability to dissolve tricalcium phosphate. It was established that the Pseudomonas migulae GEOT18 demonstrated strong phosphate-solubilizing activity (the content of free phosphorus in the culture medium was 3.25 ± 0.05 mg/ml). It was revealed that P. migulae GEOT18 synthesizes indole-3-acetic acid into the culture medium up to 21.1 ± 1.4 mg/L. Furthermore, it was established that this strain produces siderophores, can fix molecular nitrogen and does not show phytopathogenic activity. It was proved that inoculation of the marigold Tagetes patula with the P. migulae GEOT18 strain increases the biomass of plants and mass of inflorescence. It accelerates the transfer to the generative phase and prolongs the duration of flowering and fruiting in comparison with non-inoculated plants.
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