Chemical composition and diversity of lactic acid bacteria in guinea grass based silages
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Abstract
Ensilage is considered that widespread among conserving method of fodder crops. The successes of this process depend on bacteria in lactic acid bacteria (LAB) group as existing in forage crops. The chemical composition and diversity of lactic acid bacteria in silages based on guinea grass was investigated. Result showed that the silages contained 62.55 to 76.47% moisture, 23.53 to 37.45% dry matter (DM) and 6.11 to 15.74% ash. Groundnut silage (T3) showed the lowest crude fiber (CF), neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents. Two hundred and eighty-two isolates of LAB were isolated from five silage formula based on guinea grass supplementation with either lead tree leaves or groundnut. Most of isolates were gram-positive and catalase-negative bacteria. Based on morphological characteristics and PCR-RAPD analysis, the isolates were divided into 21 groups (Guinea Grass Mixed Legume Silage (GMLS) 1 to GMLS21). The phylogenetic relation of the representative strains was analyzed from the data of 16S ribosomal DNA sequencing. Prevalent of LAB found in the silages included Lactobacillus plantarum, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus hirae, and Weissella paramesenteroides. L. plantarum was a dominant population and found distribution in all silage formula. This study revealed that the silage samples, including guinea grass, lead tree and groundnut contain mainly of L. plantarum. The information obtained can be used for the anterior design of reasonable inoculants for improvement of silage quality for cattle feed.
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References
Adeneye, J. A. (1979). A note on the nutrient and mineral composition of Leucaena leucocephala in Western Nigeria. Animal Feed Science and Technology, 4:221-225.
AOAC (1995). Official methods of analysis, 16th ed. Washington DC: Association of Official Analytical Chemists, pp 69-82.
Azim, A., Khan, A. G., Nadeem, M. A. and Muhammad, D. (2000). Influence of maize and cowpea intercropping on fodder production and characteristics of silage. Asian-Australasian Journal of Animal Sciences, 3:781-784.
Babayemi, O. J. and Bamikole, M. A. (2006). Effects of Tephrosia candida DC leaf and its mixtures with guinea grass on in vitro fermentation changes as feed for ruminants in Nigeria. Pakistan Journal of Nutrition, 5:14-18.
Babayemi, O. J., Demeyer, D. and Fievez, V. (2004). Nutritive value and qualitative assessment of secondary compounds in seeds of eight tropical browse, shrub and pulse legumes. Communications in Agricultural and Applied Biological Sciences, 69:103-110.
Bamikole, M. A., Ikhatua, U. J., Arigbede, O. M., Babayemi, O. J. and Etela, I. (2004). An evaluation of the acceptability as forage of some nutritive and anti-nutritive components and of the dry matter degradation profiles of five species of ficus. Tropical Animal Health and Production, 36:157-167.
Cai, Y. (1999). Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation. Journal of Dairy Science, 82:2466-2471.
Cai, Y., Benno, Y., Ogawa, M., Ohmomo, S., Kumai, S. and Nakase, T. (1998). Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Applied and Environmental Microbiology, 64:2982-2987.
Cai, Y., Kumai, S., Ogawa, M., Benno, Y. and Nakase, T. (1999). Characterization and identification of Pediococcus species isolated from forage crops and their application for silage preparation. Applied and Environmental Microbiology, 65:2901-2906.
Cai, Y., Pang, H., Kobayashi, H., Cao, Y., Tohno, M. and Uegaki, R. (2010). Characterization of lactic acid bacteria isolated from silage. Proceeding of the 14th Asian-Australasian Association of Animal Production Societies, Chinese Society of Animal Science, Pingtung, 23-27 August 2010, pp. 1918-1921.
Daeshel, M. A., Andersson, R. E. and Fleming, H. P. (1987). Microbial ecology of fermenting plant materials. FEMS Microbiology Reviews, 46:357-367.
Ennahar, S., Cai, Y. and Fujita, Y. (2003). Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Applied and Environmental Microbiology, 69:444-451.
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Stanley, J. T. and Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology, 9th ed. Williams and Wilkins Co., Baltimore, MD. 816 p.
Kandler, O. and Weiss, N. (1986). Regular, nonsporing gram-positive rods. In: Sneath, P. H. A., Mair, N. S., Sharp, M. E. and Holt, J. G. (Eds), Bergey’s Manual of Systematic Bacteriology, Vol. 2. Williams and Wilkins CO., Baltimore, MD. pp. 1208-1234.
Kanokratana, P., Chanapan, S., Pootanakit, K. and Eurwililaichitr, L. (2004). Diversity and abundance of bacteria and archaea in the Bor Khlueng hot spring in Thailand. Journal of Basic Microbiology, 44:430-444.
Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16:111-120.
Lin, C., Bolsen, K. K., Brent, B. E. and Fung, D. Y. C. (1992). Epiphytic lactic acid bacteria succession during the pre-ensiling and ensiling periods of alfalfa and maize. Journal of Applied Bacteriology, 73:375-387.
McDonald, P., Henderson, N. and Herson, S. (1991). The Biochemistry of Silage, 2nd ed. Marlow: Chalcombe Publications. pp. 9-166.
Muhammad, I. R., Baba, M., Mustapha, A., Ahmad, M. Y. and Abdurrahman, L. S. (2008). Use of legume in the improvement of silage quality of Columbus grass (Sorghum almum Parodi). Research Journal of Animal Sciences, 2:109-112.
Mustafa, A. F., McKinnon, J. J. and Christensen, D. A. (2001). Effects of feeding ensiled spearmint (Mentha spicata) byproduct on nutrient utilization and ruminal fermentation of steers. Animal Feed Science and Technology, 92:33-43.
Ni, K., Wang, Y., Cai, Y. and Pang, H. (2015). Natural lactic acid bacteria population and silage fermentation of whole-crop wheat. Asian-Australasian Journal of Animal Sciences, 28:1123-1132.
Nigatu, A., Ahrné, S. and Molin, G. (2001). Randomly amplified polymorphic DNA (RAPD) profiles for the distinction of Lactobacillus species. Antonie van Leeuwenhoek, 79:1-6.
Nigatu, A., Ahrné, S., Gashe, B. A. and Molin, G. (1998). Randomly amplified polymorphic DNA (RAPD) for discrimination of Pediococcus pentosaceus and Ped. acidilactici and rapid grouping of Pediococcus isolates. Letters in Applied Microbiology, 26:412-416.
OECD-FAO. (2009). Food and Agriculture Organization of the United Nations. Agricultural outlook, OECD. Retrieved from http://www.oecd.org/berlin/43042301.pdf.
Oneca, M., Irigoyen, A., Ortigosa, M. and Torre, P. (2003). PCR and RAPD identification of L. plantarum strains isolated from ovine milk and cheese. Geographical distribution of strains. FEMS Microbiology Letters, 227:271-277.
Ozduven, M. L., Koç, F. and Akay, V. (2017). Effects of bacterial inoculants and enzymes on the fermentation, aerobic stability and in vitro organic matter digestibility characteristics of sunflower silages. Pakistan Journal of Nutrition, 16:22-27.
Pang, H., Qin, G., Tan, Z., Li, Z., Wang, Y. and Cai, Y. (2011a). Natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16S ribosomal RNA and recA gene analysis. Systematic and Applied Microbiology, 34:235-241.
Pang, H., Zhang, M., Qin, G., Tan, Z., Li, Z., Wang, Y. and Cai, Y. (2011b). Identification of lactic acid bacteria isolated from corn stovers. Animal Science Journal, 82:642-653.
Pérez-Pulido, R., Ben Omar, N., Abriouel, H., Lucas-López, R., Martínez-Cañamero, M. and Gálvez, A. (2005). Microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods. Applied and Environmental Microbiology, 71:7872-7879.
Pholsen, S., Khota, W., Pang, H., Higgs, D. and Cai, Y. (2016). Characterization and application of lactic acid bacteria for tropical silage preparation. Animal Science Journal, 87:1202-1211.
Polan, C. E., Stieve, D. E. and Grrett, J. L. (1998). Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia or microbial inoculants. Journal of Dairy Science, 81:765-776.
Quere, F., Deschamps, A. and Urdaci, M. C. (1997). DNA probe and PCR-specific reaction for Lactobacillus plantarum. Journal of Applied Microbiology, 82:783-790.
Rohlf, F. J. (2000). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, Version 2.20e. Exeter Publication, New York. pp.38.
Sambrook, J. and Russell, D. (2001). Molecular cloning: A laboratory manual, Vol. 1, 3rd ed. New York: Cold Spring Harbor Laboratory Press. pp. 1.55-1.58, 1.116-1.118.
Sifeeldein, A., Yuan, X., Dong, Z., Li, J., Youns, H. and Shao, T. (2018). Characterization and identification of lactic acid bacteria by 16S rRNA gene sequence and their effect on the fermentation quality of Elephant grass (Pennisetum purpureum) silage. Kafkas Universitesi Veteriner Fakultesi Dergisi, 24:123-130.
Sindhu, A. A., Khan, M. A., Mahr, U. N. and Sarwar, M. (2002). Agro-industrial by-products as a potential source of livestock feed. International Journal of Agriculture and Biology, 4:307-310.
Sneath, P. H. A. and Sokal, R. R. (1973). Numerical Taxonomy. California: Freeman. pp. 188-308.
SPSS (2008). SPSS Statistics for Windows, Version 17.0. Chicago: SPSS Inc. pp. 185-193.
Stiles, M. E. and Holzapfel, W. H. (1997). Lactic acid bacteria of foods and their current taxonomy. International Journal of Food Microbiology, 36:1-29.
Suphalucksana, W., Sangsoponjit, S. and Srikijkasemwat, K. (2017). Using leucaena to improve the quality of pineapple plant silage. Chemical Engineering Transactions, 58:847-852.
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007). MEGA4: Molecular evolutionary genetics analysis (MAGA) software version 4.0. Molecular Biology and Evolution, 24:1596-1599.
Titterton, M. and Maasdorp, B. V. (1997). Nutritional improvement of maize silage for dairying: mixed crop silages from sole and intercropped legumes and a long season variety of maize. 2. Ensilage. Animal Feed Science and Technology, 69:263-270.
Tiwari, M. R., Khanal, S., Shrestha, B. and Jha, R. K. (2006). Nutritional variation of different feed ingredients and compound feed found in different parts of Nepal. Nepal Agriculture Research Journal, 7:75-81.
Tohno, M., Kobayashi, H., Nomura, M., Kitahara, M., Ohkuma, M., Uegaki, R. and Cai, Y. (2012). Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. Animal Science Journal, 83:111-120.
Tudsri, S. (2004). Tropical Forage Crops. Bangkok: Kasetsart University Publisher. pp. 322-335.
Van Soest, P. J. and Robertson, J. B. (1979). Systems of analysis for evaluating fibrous feeds. In Pgden, W. J., Balch, C. C. and Graham, M. (Eds), Procedures of Standardization of Analytical Methodology for Feeds. IDRC, Ottawa, pp. 49-60.
Wang, S., Yuan, X., Dong, Z., Li, J., Guo, G., Bai, Y., Zhang, J. and Shao, T. (2017). Characteristics of isolated lactic acid bacteria and their effects on the silage quality. Asian-Australasian Journal of Animal Sciences, 30:819-827.
Wang, X., Haruta, S., Wang, P., Ishii, M., Igarashi, Y. and Cui, Z. (2006). Diversity of a stable enrichment culture which is useful for silage inoculant and its succession in alfalfa silage. FEMS Microbiology Ecology, 57:106-115.
Yang, J., Cao, Y., Cai, Y. and Terada, F. (2010). Natural populations of lactic acid bacteria isolated from vegetable residues and silage fermentation. Journal of Dairy Science, 93:3136-3145.
Zhang, J. G., Cai, Y., Kobayashi, R. and Kumai, S. (2000). Characteristics of lactic acid bacteria isolated from forage crops and their effects on silage fermentation. Journal of the Science of Food and Agriculture, 80:1455-1460.
