Optimization of microbial collagenolytic enzyme production by Bacillus subtilis subsp. Subtilis S13 using Plackett-Burman and response surface methodology
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Abstract
Bacillus subtilis subsp. subtilis S13, a new isolate from soil is found to be the collagenase producing bacteria. The results showed that gelatin concentration, initial pH, and incubation time were accounted for significant factors from PB design and then applied for
a central composite design (CCD) under response surface methodology (RSM) for optimization of significant factors were performed. The optimum parameters for the enhancing gelatinase production through CCD and response surface methodology were 19 g/l of pork gelatin, initial pH 6.16 for culture medium, and 59 h of incubation time, which provided the predicted maximum collatinolytic activity of 65.36 U/ml. This condition allowed approximately increasing 4-folds as compared to un-optimized condition (15 U/ml). The obtained optimal activity of this enzyme might be expressed the potential collagenase for several applications including meat tenderizing enzyme.
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References
Akiyama, K., Mori, K. and Takata, R. (1999). Identification of collagenase produce by Bacillus cereus R75E isolated from human colostrum Journal of Bioscience and Bioengineering, 87:231-233.
Beg, Q. K. and Gupta, R. (2003). Purification and characterization of an oxidation stable, thiol-dependent serine alkaline protease from Bacillus mojavensis. Enzyme and Microbial Technology, 32:294-304.
Çalık, P., Çalık, G. and Özdamar, T. H. (2001). Bioprocess development for serine alkaline protease production: A Review. Reviews in Chemical Engineering, 17:1-62.
Chu, I. M., Lee, C. and Li, T.S. (1992). Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416. Enzyme and Microbial Technology, 14:755-761.
Dean, A. M. and Voss, D. (2000). Design and analysis of experiments. New York (NY): Springer-Verlag New York Inc.
Deutscher, J. (2008). The mechanisms of carbon catabolite repression in bacteria. Current Opinion in Microbiology, 11:87-93.
Gupta, R., Beg, Q. K. and Lorenz, P. (2002a). Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology, 59:15-32.
Gupta, R., Beg, Q. K., Khan, S. and Chauhan, B. (2002b). An overview on fermentation, downstream processing and properties of microbial alkaline proteases. Applied Microbiology and Biotechnology, 60:381-395.
Huang, G. L., Gosschalk, J. E., Kim, Y. S., Ogorzalek, Loo, R. R. and Clubb, R. T. (2018). Stabilizing displayed proteins on vegetative Bacillus subtilis cells. Applied Microbiology and Biotechnology, 102:6547-6565.
Johnvesly, B. and Naik, G. R. (2001). Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium. Process Biochemistry, 37:139-144.
Kawahara, H., Kusumoto, M. and Obata, H. (1993). Isolation and characterization of a new type of collagenase producing bacterium, Bacillus alvei DC-1 Bioscience Biotechnology and Biochemistry, 57:1372-1373.
Lama, L., Romano, I., Calandrelli, V., Nicolaus, B. and Gambacorta, A. (2005). Purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the Salinivibrio genus. Research in Microbiology, 156:478-484.
Liu, S., Fang, Y., Lv, M., Wang, S. and Chen, L. (2010). Optimization of the production of organic solvent-stable protease by Bacillus sphaericus DS11 with response surface methodology. Bioresource Technology, 101:7924-7929.
Mabrouk, S. S., Hashem, A. M., El-Shayeb, N. M. A., Ismail, M. S. and Abdel-Fattah, A. F. (1999). Optimization of alkaline protease productivityby Bacillus licheniformis ATCC 21415. Bioresource Technology, 69:155-159.
Moon, S. H. and Parulekar, S. J. (1993). Some observations on protease production in continuous suspension cultures of Bacillus firmus. Biotechnology and Bioengineering, 41:43-54.
Nagano, H. and To, K. A. (1999). Purification of collagenase and specificity of its releted enzyme from Bacillus subtillis FS-2. Bioscience, Biotechnology, and Biochemistry, 63:181-183.
Nielsen, J. and Villadsen, J. (1994). Bioreaction engineering principles. Plenium Press, New York, pp. 456.
Oskouie, S. F. G, Tabandeh, F., Yakhchali, B. and Eftekhar, F. (2008). Response surface optimization of medium composition for alkaline protease production by Bacillus clausii. Biochemical Engineering Journal, 39:37-42.
Pal, G. K and Suresh, P. V. (2016). Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition. Royal Society of Chemistry, 6:33763-33780.
Patel, R., Dodia, M. and Singh, S. P. (2005). Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: production and optimization. Process Biochemistry, 40:3569-3575.
Priest, F. G. (1977). Extracellular enzyme synthesis in the genus Bacillus. Bacteriological Reviews, 41:711-753.
Priya, P. P., Mandge, S. and Archana, G. (2011). Statistical optimization of production and tannery applications of a keratinolytic serine protease from Bacillus subtilis P13. Process Biochemistry, 46:1110-1117.
Reddy, L. V. A., Wee, Y. J., Yun, J. S. and Ryu, H. W. (2008). Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett–Burman and response surface methodological approaches. Bioresource Technology, 99:2242-2249.
Rui, R., Zhiqiang, L. and Min, C. (2009). Isolation and purification of caseinase and collagenase from commercial Bacillus subtilis AS1.398 enzyme by affinity chromatography. Journal of the Society of Leather Technologists and Chemists, 93:8-11.
Sai-Ut, S., Benjakul, S., Sumpavapol, P. and Kishimura, H. (2014). Optimization of gelatinolytic enzyme productionby B. amyloliquefaciens sp. H11 through Plackett–Burmandesign and response surface methodology. Food Science and Biotechnology, 22:2008-4935.
Sela, S., Schickler, H., Chet, I. and Spiegel, Y. (1998). Purification and characterization of a Bacillus cereus collagenolytic/proteolytic enzyme and its effect on Meloidogyne Javanica Cuticular Proteins. European Journal of Plant Pathology, 104:59-67.
Sorapukdee, S. and Tangwatcharin, P. (2019). Collagenolytic Enzymes from Selected Bacteria and Its Potential Application in Beef Tenderization. Progress report to the Thailand ResearchFund (TRF) through Research Grant for New Scholar (MRG6180240). (unpubished data).
Suphatharaprateep, W., Cheirsilp, B. and Jongjareonrak, A. (2011). Production and properties of two collagenases from bacteria and their application for collagen extraction. New Biotechnology, 28:649-655.
Tran, I. H. and Nagano, H. (2002). Isolation and characteristic of Bacillus subtilis CN2 and its collagenase production. Journal of Food Science, 67:1184-1187.
Wu, Q., Li, C., Li, C., Chen, H. and Shuliang, L. (2010). Purification and Characterization of a Novel Collagenase from Bacillus pumilus CoI-J. Applied Biochemistry and Biotechnology, 160:129-139.
Yang, F., Long, L., Sun, X., Wu, H., Li, T. and Xiang, W. (2014). Optimization of medium using response surface methodology for lipid production by Scenedesmus sp. Marine Drugs, 12:1245-1257.
