Paternity testing of crossbred beef cattle with 15 microsatellite markers
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Abstract
Alleles from only 9 microsatellites were detected as BM1818, BM1824, BM2113, CSSM66, ETH10, ETH225, INRA023, INRA037, and INRA063. The highest number of alleles (23 alleles) was found in CSSM66, while the lowest number (13 alleles) was found in ETH 10. BM1818 had expressed an allele size between 239 to 316 bp, while the smallest sizes, 129 to 179 bp, were detected in BM2113. Twenty-nine pairs of sire-offspring obtained a positive LOD score, whereas B-066 with Carlos-sire obtained a negative LOD score. It meant the 29 pairs of sire-offspring were matched with the pedigree records. These 9 microstellites could be used for paternity testing.
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References
Cervini, M., Henrique-Silva, F., Mortari, N. and Matheucci, E. Jr. (2006). Genetic variability of 10 microsatellite markers in the characterization of Brazilian Nellore cattle (Bos indicus). Genetic and Molecular Biology, 29:486-490.
Curi, R. A. and Lopes, C. R. (2002). Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines. Brazilian Journal of Veterinary Research and Animal Science, 39:129-135.
Escobar, C.H., Ángel, M. O., Alfonso, H. O. and Guerra, M. T. (2009). Genetic variability of the Zebu cattle breed (Bos indicus) in the Department of Huila, Colombia using microsatellite molecular markers. Acta Biológica Colombiana, 14:173-180.
FAO (2011). Molecular genetic characterization of animal genetic resources. FAO Animal Production and Health Guidelines. No. 9. Rome, pp. 68-69.
ICT (2018). Retrieved from http://ict.dld.go.th/webnew/index.php/th/service-ict/report/310-report-thailand-livestock/reportservey2561/1293-2561-country.
Kalinowski, S. T., Taper, M. L. and Marshall, T. C. (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16:1099-1106.
Marshall, T. C., Slate, J., Kruuk, L. E. B. and Pemberton, J. M. (1998). Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology, 7:639-655.
Peelman, L. J., Mortiaux, F., Van Zeveren, A., Dansercoer, A., Mommens, G., Coopman, F., Bouquet, Y., Burny, A., Renaville, R. and Portelle, D. (1998). Evaluation of the genetic variability of 23 bovine microsatellite markers in four Belgian cattle breeds. Animal Genetics, 29:161-167.
Pei, J., Bao, P., Chu, M., Liang, C., Ding, X., Wang, H., Wu, X., Guo, X. and Yan, P. (2018). Evaluation of 17 microsatellite markers for parentage testing and individual identification of domestic yak (Bos grunniens). Peer-reviewed scientific mega journal Retrieved from https://peerj.com/articles/5946/.
Pemberton, J. M. (2008). Wild pedigrees: The way forward. Proceedings of the Royal Society of London Series B, 275:613-621.
Stevanovic, J., Stanimirovic, Z., Dimitrijevic, V. and Maletic, M. (2010). Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). Czech Journal of Animal Science, 55:221-226.
Tian, F., Sun, D. and Zhang, Y. (2008). Establishment of paternity testing system using microsatellite markers in Chinese Holstein. Journal of Genetics and Genomics, 35:279-284.
Van Eenennaam, A. L., Weaber, R. L., Drake, D. J., Penedo, M. C. T., Quaas, R. L., Garrick, D. J. and Pollak, E. J. (2007). DNA-based paternity analysis and genetic evaluation in a large, commercial cattle ranch setting. Journal of Animal Science, 85:3159-3169.
Yoon, D. H., Kong, H. S., Oh, J. D., Lee, J. H., Cho, B. W., Kim, J. D., Jeon, K. J., Jo, C. Y., Jeon, G. J. and Lee, H. K. (2005). Establishment of an Individual Identification System Based on Microsatellite Polymorphisms in Korean Cattle (Hanwoo). Asian Australasian Journal of Animal Sciences, 18:762-766.
Zhang, Y., Wang, Y., Sun, D., Yu, Y. and Zhang, Y. (2010). Validation of 17 Microstellite Markers for Parentage Verification and Identity Test in Chinese Holstein Cattle. Asian Australasian Journal of Animal Sciences, 23:425-429.
