Effect of glucose on endoglucanase and β-glucosidase production by some indigenous Indonesian fungi
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Abstract
The production of polysaccharide-degrading enzymes in most fungi is affected by carbon catabolite repression. The effect of glucose on the production of endoglucanase and β-glucosidase was examined by some indigenous Indonesian fungi. After screening for cellulase production using the clear zone method in solid medium, 11 indigenous fungi isolated from various sources in Indonesia were grown in liquid medium containing carboxymethyl cellulose as a carbon source and supplemented with glucose at various concentrations (0, 1, 3, 5%) for 4 days. Endoglucanase and β-glucosidase activities in the growth medium were then examined. Strains PK1J2, MLT3J2, MLT4J1, and MLT5J1 produced more endoglucanase and β-glucosidase than the other strains in medium without glucose supplementation. Trichoderma asperellum PK1J2 was the highest enzyme producer, giving 0.79 U/mL endoglucanase and 1.49 U/mL β-glucosidase. Except for strains MLT2J2, MLT5J1 and G2J2, endoglucanase and β-glucosidase production by the other strains was partially repressed by 1% glucose. However, endoglucanase and β-glucosidase were hardly produced by all strains when the fungi were grown in medium supplemented with 3% glucose
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