Determination of pregnancy associated - glycoproteins (PAGs) During and post pregnancy in riverine buffaloes (Bubalus bubalis Linn.)
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Abstract
Through pregnancy-associated glycoprotein assay, an overall conception rate of 54. 17% at days 25 and 30 post fixed-time artificial insemination (FTAI) was observed in the study. The pregnancy of the riverine buffaloes (n=13) was confirmed through transrectal ultrasonography (TRUS) at day 40, showing the presence of an amniotic vesicle and an embryo with a beating heart. The mean plasma level of pregnancy-associated glycoproteins (PAGs) of the pregnant buffaloes (n=11) were found at a high level as early as days 25, 30, and 40 (1.21 ± 0.20 ng/ml, 12.11 ± 1.67, and 28.81 ± 2.57 ng/ml) and observed to have increasing trend/pattern with the progression of pregnancy until day 300 (114.01 ± 10.05). Two of the animals that were confirmed pregnant at day 40 post artificial insemination (AI) via TRUS undergone early pregnancy loss. While the non-pregnant buffalo’s plasma PAGs remained at a very low level on day 25 (0.17 ± 0. 04 ng/ml) to day 40 (0.06 ± 0.02 ng/ml) and further confirmed the non-pregnancy via TRUS. Two waves of peak increase in the concentration of PAGs were found during the gestation period of the riverine buffaloes. The 1st peak was observed during the 1st trimester (day 60) and the 2nd peak was during the last trimester (day 270) of gestation. Generally, the findings on PAGs at early and throughout the gestation period of riverine buffaloes appeared higher than those reported in other studies. Postpartum residual clearance of PAGs concentration in the maternal circulation of the buffaloes was found to be slowly decreasing from 114.33 ± 13.75ng/ml at week 1 to nadir at week 10 with a plasma level of 0.11 ± 0.01 ng/ml.
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